Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity

Byon, Yun Young; Kim, Mi Hyoung; Yoo, Eun Sook; Hwang, Kyu Kye; Jee, Youngheun; Shin, Taekyun; Joo, Hong Gu

doi:10.4142/jvs.2008.9.4.359

Cited by 34 publications

(23 citation statements)

References 21 publications

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“…Fucoidan was recently reported to have beneficial effects on the cell differentiation and mineralization of osteoblastic cells and radioprotective effects on bone marrow cells (Byon et al 2008;Cho et al 2009;Park et al 2012). Fucoidan was reported to stimulate the antigen-presenting functions of dendritic cells and increase the viability of bone marrow cells by inhibiting radiation-induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Fucoidan Induces Cell Aggregation and Apoptosis in Osteosarcoma MG-63 Cells

Kim

Jeon

2016

Animal Cells and Systems

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Fucoidan, a sulfated polysaccharide found in brown algae, possesses various biological activities including anti-inflammatory and anti-cancer effects. In this study, we investigated the effects of fucoidan on the cell growth and morphology of human osteosarcoma MG-63 cells and found that fucoidan induces cell aggregation and apoptosis in osteosarcoma cells when the cells are treated with fucoidan upon seeding before becoming stably attached to the plates. Typical characteristics of apoptotic cells such as chromatin condensation and nuclear fragmentation were observed in fucoidan-treated cells. The number of annexin V-stained cells was increased by fucoidan treatment in a dose-dependent manner. Consistent with these results, cell viability and growth rate were decreased and cell spreading was inhibited in the presence of fucoidan, leading to rounded morphological changes of the cells. Our results demonstrate that fucoidan treatment results in cell aggregation through F-actin accumulation at the rounded cell cortex and apoptosis in osteosarcoma MG-63 cells.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Fucoidan Induces Cell Aggregation and Apoptosis in Osteosarcoma MG-63 Cells

Kim

Jeon

2016

Animal Cells and Systems

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“…Previous studies have shown that it protects bone marrow cells from radiation [1] and inhibits selectin-mediated adhesion of hematopoietic progenitor cells [2]. Also, fucoidan modulates the function of various immune cells such as lymphocytes [3], dendritic cells (DCs) [4], natural killer (NK) cells [5], and neutrophils [6].…”

Section: Introductionmentioning

confidence: 99%

Fucoidan Enhances the Survival and Sustains the Number of Splenic Dendritic Cells in Mouse Endotoxemia

Ko¹,

Joo

2011

Korean J Physiol Pharmacol

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Fucoidan is a sulfated polysaccharide derived from brown algae that has been reported to perform multiple biological activities, including immunostimulation. In this study, we investigated whether fucoidan has beneficial effects on endotoxemia induced by LPS, a septic model in mice. The focus of this study was on survival rates and spleen function of the mice upon treatment. W e found that fucoidan had prophylactic effects on the survival rate of mice with endotoxemia. Flow cytometric analysis using antibodies for subset-specific markers revealed that fucoidan profoundly reversed the depleted population of dendritic cells in mice with endotoxemia. According to W estern blot analysis, the spleen cells of LPS/fucoidan-treated mice showed a higher expression of anti-apoptotic molecules compared to those of LPS-treated mice. Also, fucoidan-treated spleen cells were more responsive to mitogens. Taken together, these results demonstrate that fucoidan pre-treatment has beneficial effects on the survival rate and function of the spleen in mice with endotoxemia. This study may broaden the use of fucoidan in clinical fields, especially endotoxemia.

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“…Fucoidans have a variety of biological functions (20), including mobilization of progenitor cells (21), stimulation of dendritic cells and radioprotection of bone marrow cells (22). A wide range of findings led to studies on proviral loads in humans with T-lymphotropic virus type-1.…”

Section: Administered Samples Affect Interleukin (Il)-10 (A) and Tumentioning

confidence: 99%

Fucoidans Stimulate Immune Reaction and Suppress Cancer Growth

Vĕtvická

Větvičková

2017

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Abstract. Background Fucoidan is a complex sulfated polysaccharide consisting of sulfated fucose residues and found mostly in brown marine algae, echinoderms (1) and seagrasses (2). Fucoidans exhibit numerous biological activities found both in in vitro and in vivo experiments. Fucoidan extracts have been found to ameliorate acute colitis (3), have anticancer activity (4) mediate via natural killer (NK) cells (5) and affect inflammation, vascular physiology, as well as oxidative stress (6, 7). Some effects differ based on the type of extract, as documented by the differential effects of high and low molecular weight fucoidans on the severity of arthritis in mice. The low molecular weight samples reduced arthritis via suppression of Th1-mediated immunity, whereas high molecular weight samples enhanced the inflammatory activation of macrophages (8). Subsequent experiments revealed that high molecular weight fucoidans stimulated spleen cells, whereas low molecular weight samples had little activity or were toxic (9). The reasons for these differences are still unknown. Using a fish model, however, dietary fucoidan was found to influence blood chemistry and constituents, antioxidation and innate immunity (10). In human models, fucoidan delayed apoptosis and induced proinflammatory cytokines, probably via activation of the PI3K/AKT pathway (11). As fucoidan can function as an adjuvant and stimulate antibody response (12), it has been used to improve vaccine efficacy (13).Cancer development is the most studied effect of fucoidan. The mechanisms are still unknown. However, fucoidan can directly induce cytotoxicity and apoptosis of cancer cells (14) probably explained by an additional study suggesting implication of macrophages and blood leukocytes in relation to fucoidan (15).In our study, we not only directly compared the biological activities of individual fucoidan samples, but also compared purified samples with commercially available component mixtures. Thus, our study answers the question not only whether fucoidans influence immune reactions, but also if the popular idea of combining biologically active molecules together improves their effectiveness. Materials and MethodsMaterials. Individual samples were purchased from the manufacturers or distributors as shown in Table I. RPMI 1640 medium, sodium citrate, 4-(2-hydroxyethyl)-1-piperazine-ethanol-sulfonic acid (HEPES), antibiotics, Wright stain, Freund's adjuvant, ovalbumin, Concanavalin A (Con A) and lipopolysaccharide (LPS) were purchased from Sigma (St. Louis, MO, USA). Fetal calf serum (FCS) was from Hyclone Laboratories (Logan, UT, USA).Cells. J774A.1 mouse macrophages and YAC cells were obtained from the ATCC (Manassas, VA, USA). The BALB/c mouse-derived mammary tumor cell line Ptac64 was generously provided by Dr. Wei-Zen Wei of the Michigan Cancer Foundation, Wayne State University, Detroit, MI, USA. The cell lines were maintained in RPMI 1640 medium containing HEPES buffer supplemented with 10% heat-inactivated FCS, 100 U/ml penicillin and 100 μg/ml stre...

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Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity

Cited by 34 publications

References 21 publications

Fucoidan Induces Cell Aggregation and Apoptosis in Osteosarcoma MG-63 Cells

Fucoidan Induces Cell Aggregation and Apoptosis in Osteosarcoma MG-63 Cells

Fucoidan Enhances the Survival and Sustains the Number of Splenic Dendritic Cells in Mouse Endotoxemia

Fucoidans Stimulate Immune Reaction and Suppress Cancer Growth

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