Radioprotective efficacy of zinc oxide nanoparticles on γ-ray-induced nuclear DNA damage in Vicia faba L. as evaluated by DNA bioassays

Mohamed, Ekram Abdelhaliem; Harbi, Hanan Fahad Al; Aref, Nagwa

doi:10.1080/16878507.2019.1690798

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Gamma rays are the most effective ionizing radiation used in agricultural programs, for instance, for the surface sterilization of agricultural products in order to increase their conservation time via reducing pathogen propagation. The effects of γ-radiation on the nuclear DNA of Vicia faba seeds either without treatment or treated with ZnO nanoparticles were studied using flow cytometry, an alkaline Comet assay, and the random amplification of polymorphic DNA (RAPD)-PCR; all three assays confirmed that the treatment of the seeds with ZnO nanoparticles preserved their DNA integrity [159]. Furthermore, γ-radiation is an attractive alternative to the chemicals used against an obligate endoparasite, the root-knot nematode Meloidogyne incognita that can infect most vegetables, fruits, and ornamental plants all over the world [160].…”

Section: Use Of the Comet Assay In Studies On Plants Exposed To Genot...mentioning

confidence: 96%

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Tyutereva,

Strizhenok,

Kiseleva

et al. 2024

Horticulturae

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Contrarily to chronic stresses, acute (i.e., fast and dramatic) changes in environmental factors like temperature, radiation, concentration of toxic substances, or pathogen attack often lead to DNA damage. Some of the stress factors are genotoxic, i.e., they damage the DNA via physical interactions or via interference with DNA replication/repair machinery. However, cytotoxic factors, i.e., those that do not directly damage the DNA, can lead to secondary genotoxic effects either via the induction of the production of reactive oxygen, carbon, or nitrogen species, or via the activation of programmed cell death and related endonucleases. The extent of this damage, as well as the ability of the cell to repair it, represent a significant part of plant stress responses. Information about DNA damage is important for physiological studies as it helps to understand the complex adaptive responses of plants and even to predict the outcome of the plant’s exposure to acute stress. Single cell gel electrophoresis (Comet assay) provides a convenient and relatively inexpensive tool to evaluate DNA strand breaks in the different organs of higher plants, as well as in unicellular algae. Comet assays are widely used in ecotoxicology and biomonitoring applications; however, they are still relatively rarely used in physiological studies. In this review, we provide an overview of the basic principles and of useful variations of the protocols of Comet assays, as well as of their use in plant studies, in order to encourage plant physiologists to include this tool in the analysis of plant stress responses.

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Section: Use Of the Comet Assay In Studies On Plants Exposed To Genot...mentioning

confidence: 96%

Comet Assay: Multifaceted Options for Studies of Plant Stress Response

Tyutereva,

Strizhenok,

Kiseleva

et al. 2024

Horticulturae

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“…If we talk about perspectives in medicine, the radiation protection effects of NPs, such as ZnO, carbon, characterized by strong antioxidant properties, have an important role in protecting cells against DNA damage induced by ionizing radiation. The excellent biocompatibility of some metal oxides, ZnO NPs are among the most popular oxide, NPs in biological applications due to their excellent biocompatibility, economic, and low toxicity [50].…”

Section: New Perspectivesmentioning

confidence: 99%

Additive Manufacturing as a Means of Gas Sensor Development for Battery Health Monitoring

et al. 2021

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Lithium-ion batteries (LIBs) still need continuous safety monitoring based on their intrinsic properties, as well as due to the increase in their sizes and device requirements. The main causes of fires and explosions in LIBs are heat leakage and the presence of highly inflammable components. Therefore, it is necessary to improve the safety of the batteries by preventing the generation of these gases and/or their early detection with sensors. The improvement of such safety sensors requires new approaches in their manufacturing. There is a growing role for research of nanostructured sensor’s durability in the field of ionizing radiation that also can induce structural changes in the LIB’s component materials, thus contributing to the elucidation of fundamental physicochemical processes; catalytic reactions or inhibitions of the chemical reactions on which the work of the sensors is based. A current method widely used in various fields, Direct Ink Writing (DIW), has been used to manufacture heterostructures of Al2O3/CuO and CuO:Fe2O3, followed by an additional ALD and thermal annealing step. The detection properties of these 3D-DIW printed heterostructures showed responses to 1,3-dioxolan (DOL), 1,2-dimethoxyethane (DME) vapors, as well as to typically used LIB electrolytes containing LiTFSI and LiNO3 salts in a mixture of DOL:DME, as well also to LiPF6 salts in a mixture of ethylene carbonate (EC) and dimethyl carbonate (DMC) at operating temperatures of 200 °C–350 °C with relatively high responses. The combination of the possibility to detect electrolyte vapors used in LIBs and size control by the 3D-DIW printing method makes these heterostructures extremely attractive in controlling the safety of batteries.

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