Radiosensitivity in HeLa cervical cancer cells overexpressing glutathione S-transferase π 1

Yang, Liang-Tung; Liu, Ren; Ma, Hongbing; Mao, Ying; Wang, Yajie

doi:10.3892/ol.2015.3394

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…In addition, GSTP1 is associated with resistance of therapeutic drugs in tumors. Increased expression of GSTP1 inhibits radiosensitivity in HeLa cells 31 . GSTP1 increases breast cancer risk and aggressiveness but enhances response to cyclophosphamide chemotherapy in North China 32 .…”

Section: Discussionmentioning

confidence: 99%

FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression

Wang

Yan

et al. 2019

Cell Death Dis

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F-box only protein 8 (FBX8), as a critical component of the SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases, has been associated with several malignancies through interacting with a member of proteins. However, the substrates of FBX8 for destruction in the progression of colorectal carcinoma (CRC) need to be explored. Here, we show that loss of FBX8 accelerates chemical-induced colon tumorigenesis. FBX8 directly targets GSTP1 for ubiquitin-mediated proteasome degradation in CRC. GSTP1 promotes the proliferation, invasion, and metastasis of CRC cells. Furthermore, GSTP1 is upregulated in CRC tissue samples and predicts poor prognosis of CRC patients. The inactivation of FBX8 negatively correlated with increased levels and stability of GSTP1 in clinical CRC tissues and FBX8 knockout transgenic mice. These findings identify a novel ubiquitination pathway as FBX8-GSTP1 axis that regulates the progression of CRC, which might be a potential prognostic biomarker for CRC patients.

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Section: Discussionmentioning

confidence: 99%

FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression

Wang

Yan

et al. 2019

Cell Death Dis

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“…Treatment of HeLa cells with different concentrations of TCE led to a concentration dependent decline in the GST activity and irradiation of TCE treated cells caused a further decline in the GST activity which may be one of the reasons of reduced cell survival in the TCE+irradiation group, when compared to MEM+irradiation group. The expression of GST has been correlated with high cell proliferation and reduced radiosensitivity in HeLa cells [56]. Hematoporphyrin dimethyl ether has been reported to increase the radiosensitivity of Ehrlich ascites carcinoma by reducing GST activity earlier [57].…”

Section: Discussionmentioning

confidence: 99%

Dichloromethane Extract of Giloe (Tinospora Cordifolia, Wild) Increases the Radiosensitivity of Cultured Hela Cells Exposed to Different Doses of Γ-Radiation

Gc¹

2017

JONAM

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Ionizing radiations are in frequent clinical use to treat cancer either alone or in combination with surgery or chemotherapy. The radioresistance of tumors is a stumbling block to realize the full potential of radiotherapy. Therefore, pharmacophores that reduce the radioresistance of tumors may be of great importance during tumor therapy. In the present study an attempt has been made to evaluate the potential of dichloromethane extract of giloe i.e. Tinospora cordifolia (TCE) in cultured HeLa cells. Exposure of HeLa cells to TCE for 4 h before exposure to 2 Gy γ-radiation led to a significant decrease in the cell viability (approximately 50 %) accompanied by a reduction in the surviving fraction (SF) up to 0.52 after 4 h of TCE treatment. Thereafter, clonogenecity of HeLa cells declined negligibly with increase in treatment duration up to 6 h post-treatment. There has been a dose dependent attrition in the cell viability of HeLa cells exposed to 1-4 Gy γ-irradiation, whereas treatment of HeLa cells with various doses of TCE further decreased the cell viability depending not only on the irradiation dose but also on the concentration of TCE. The irradiation of HeLa cells resulted in a radiation dose dependent decline in the SF and increasing TCE concentration before irradiation caused a further TCE concentration dependent reduction in SF, and a lowest SF was observed for 4 µg/ml TCE for all irradiation doses. Treatment of HeLa cells with different concentrations of TCE before 3 Gy irradiation resulted in a concentration dependent depletion in glutathione-S-transferase activity until 12 h post-irradiation, whereas lactate dehydrogenase and lipid peroxidation increased up to 4 h post-irradiation and declined gradually thereafter up to 12 h post-irradiation. The TCE treatment increased the radiosensitivity of HeLa cells by reducing glutathione-S-transferase activity and increasing the activity of lactate dehydrogenase and lipid peroxidation.

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“…This study utilized the HeLa cell, which is a cell type in immortal human cell line and used commonly in biochemical and medical science. [22][23][24] The cells were cultivated in a cell culture dish (Φ35) (2-8590-01, AS ONE Corp., Japan) containing 2 ml (1.5 × 10 5 cells/ml) with a regular medium that consists of Dulbecco's modified Eagle medium (D-MEM 043-30085, Wako Pure Chemical Industries, Ltd., Japan), 10% fetal bovine serum (FBS, SH30910.03, HyClone Laboratories, Inc., United States), and 1% penicillin-streptomycin (168-23191, Wako Pure Chemical Industries Ltd., Japan) for 24 h in an incubator (WKN-9100EX, Waken B Tech Co., Ltd., Japan) at 37°C with 5% CO 2. Figure 1 is a schematic diagram of the experimental setup of DPJ and MPJ generation.…”

Section: A Cell Cultivationmentioning

confidence: 99%

Generation of Atmospheric Pressure Dry-and Mist-Plasma Jets and Their Effects on HeLa Cells

et al. 2015

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Atmospheric pressure plasma jets have recently received significant attention due to their unique capabilities and novel applications. Various chemical species, such as NO 2 , HNO 3 , O 3 , and OH, generated in air by plasma are dissolved and transported in water quickly, and accompanied chemical stimuli can inactivate bacteria. In our previous study, focusing on OH and H 2 O 2 production, we developed a mist-plasma jet (MPJ) generated using dry helium gas mixed with pure water mist as an alternative to the traditional method using only dry helium gas, known as the dry-plasma jet (DPJ). In this study, the observation and comparison of effects of both MPJ and DPJ on HeLa cells surrounded by cell culture medium immediately after irradiation by plasma and following 24 h were focused. In addition, to observe details of effects of both plasma jets on HeLa cells, two experimental procedures were prepared. One is that of irradiated culture medium, including that with cells and observed cells replaced with fresh cell culture medium following 24 h. The other is that of irradiated culture medium, without cells, with plasma, and observed cells exposed to the plasma-treated culture medium after 24 h. These experiments revealed that MPJ more greatly influences cell death than DPJ.

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Radiosensitivity in HeLa cervical cancer cells overexpressing glutathione S-transferase π 1

Cited by 6 publications

References 17 publications

FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression

FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression

Dichloromethane Extract of Giloe (Tinospora Cordifolia, Wild) Increases the Radiosensitivity of Cultured Hela Cells Exposed to Different Doses of Γ-Radiation

Generation of Atmospheric Pressure Dry-and Mist-Plasma Jets and Their Effects on HeLa Cells

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