Radiotracer methods and neutron activation analysis for the investigation of nanoparticle biokinetics in living organisms

Antsiferova, A. A.; Buzulukov, Yu. P.; Демин, В. А.; Демин, В. Ф.; Рогаткин, Д. А.; Petritskaya, E. N.; Abaeva, L. F.; Кашкаров, П. К.

doi:10.1134/s1995078015010024

Cited by 17 publications

(19 citation statements)

References 12 publications

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“…This was shown in many in vitro and in vivo studies [ 6 , 7 , 8 , 9 , 10 ]. Recently, in in-vitro experiments, it was demonstrated that silver nanoparticles may cross the blood-brain barrier (BBB) of mammals, which protects the brain from toxic substances, and accumulate there [ 11 , 12 ]. Moreover, Ag-NPs are difficult to eliminate from the brain, which may be due to the low exocytosis level and low number of immune cells [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Prolonged Silver Nanoparticle Exposure on the Contextual Cognition and Behavior of Mammals

2018

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Silver nanoparticles have been widely used in the lighting and food industries, in medicine, and in pharmaceutics as an antiseptic agent. Recent research demonstrates that, after prolonged oral administration, silver nanoparticles may cross the blood-brain barrier and accumulate in the brain in rather high amounts. In ex vivo experiments, it has also been shown that silver nanoparticles demonstrate neurotoxicity. The objective of this work was to answer the questions whether silver nanoparticles change cognitive and behavioral functions of mammals after prolonged administration if silver nanoparticles have accumulated in the brain. C57Bl/6 male mice were orally exposed to PVP-coated silver nanoparticles daily for 30, 60, 120 and 180 days. Control mice were exposed to distilled water. After that they were tested in the Open Field, Elevated Plus Maze, Light-Dark Box and contextual fear conditioning task. The data have shown that the experimental mice went through three periods of switching in the behavior caused by adaptation to the toxic silver nanoparticles: anxiety, appearance of research instinct and impairment of long-term memory. This provides evidence of the hazardous effect of silver nanoparticles, which appears after long periods of silver nanoparticle oral administration.

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Section: Introductionmentioning

confidence: 99%

Effects of Prolonged Silver Nanoparticle Exposure on the Contextual Cognition and Behavior of Mammals

2018

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“…The mice received the solution in their drinking water. The dose was chosen as 1 2 of the previously used dose for NAA studies [19,20]. This allows tracing of the accumulated amount of NPs in the different regions of the brain and ensures the mice do not die.…”

Section: Cognitive Testmentioning

confidence: 99%

“…During evolution, mechanisms developed to address natural nanosized structures such as proteins and cellular organelles. For instance, a living cell can recognize proteins in the native state [ 19 ], which are always nanosized, as solid or liquid food and take them up. Therefore, engineered nanoparticles with or without a corona of proteins can be regarded as food or construction materials for the cell.…”

Section: Introductionmentioning

confidence: 99%

Disturbance in Mammalian Cognition Caused by Accumulation of Silver in Brain

Antsiferova

Kopaeva

Kochkin

et al. 2021

Toxics

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The influence of daily prolonged administration of silver nanoparticles on the cognitive functions of a model mammal was studied. The accumulation of silver in the whole brain and the hippocampus, cerebellum, cortex and residual brain tissue of the mouse was investigated by highly precise and representative neutron activation analysis, and histological studies were conducted. Here, we show that long-term memory impairments were caused by the accumulation of silver nanoparticles in the brain and its subregions, such as the hippocampus, cerebellum and cortex, in a step-like manner by disturbance of hippocampal cell integrity. Three different approaches allowed us to observe this phenomenon and discover the reasons it occurred.

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“…The amounts of silver accumulated in brains and their parts were studied by not so wide-spread technique for this purpose as NAA. It required several years to develop the approach of the best tracking, detecting and precise measuring of the amounts of each kind of nanoparticles, for instance, silver, in biological tissues [22,44,45]. However, the principle of NAA is fairly simple.…”

Section: Neutron Activation Analysismentioning

confidence: 99%

“…For instance, in some cases Ag-NPs can accumulate in different organs such as liver, kidney, blood and brain of mammals [22][23][24][25]. Several works showed that all tissues except the brain easily remove silver [23,24] and some scientific researches reliably demonstrate the penetration of Ag-NPs via the blood-brain barrier and their jamming in the nerve tissue [23,24,26].…”

Section: Introductionmentioning

confidence: 99%

Accumulation of Silver Nanoparticles in Mice Brain Parts and the Harmful Effects

Antsiferova¹,

Kopaeva²,

Kochkin³

et al. 2019

J Nanomed Nanotechnol

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Radiotracer methods and neutron activation analysis for the investigation of nanoparticle biokinetics in living organisms

Cited by 17 publications

References 12 publications

Effects of Prolonged Silver Nanoparticle Exposure on the Contextual Cognition and Behavior of Mammals

Effects of Prolonged Silver Nanoparticle Exposure on the Contextual Cognition and Behavior of Mammals

Disturbance in Mammalian Cognition Caused by Accumulation of Silver in Brain

Accumulation of Silver Nanoparticles in Mice Brain Parts and the Harmful Effects

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