Radix Angelica Sinensis and Radix Hedysari Ultrafiltration Extract Protects against X‐Irradiation‐Induced Cardiac Fibrosis in Rats

Abstract

Radiation-induced myocardial fibrosis (RIMF) is the main pathological change associated with radiation-induced heart toxicity after radiation therapy in patients with thoracic tumors. There is an antifibrosis effect of Radix Angelica Sinensis and Radix Hedysari (RAS-RH) ultrafiltration extract from Danggui Buxue decoction (DBD) in X-irradiation-induced rat myocardial fibrosis, and this study aimed to investigate whether that effect correlated with apoptosis and oxidative stress damage in primary rat cardiac fibroblasts; further, the potential mechanisms were also explored. In this study, we first found that the RAS-RH antifibrosis effect was associated with the upregulation of microRNA-200a and the downregulation of TGF-β1/smad3 and COL1α. In addition, we also found that the antifibrosis effect of RAS-RH was related to the induction of apoptosis in primary rat cardiac fibroblasts and to the prevention of damage caused by reactive oxygen species (ROS). Interestingly, primary rat cardiac fibroblasts exposed to X-ray radiation underwent apoptosis less frequently in the absence of RAS-RH. Therefore, RAS-RH has the ability to protect against fibrosis, which could be occurring through the induction of apoptosis and the resistance to oxidative stress in rats with X-irradiation-induced myocardial fibrosis; thus, in a model of RIMF, RAS-RH acts against X-irradiation-induced cardiac toxicity.

Role and molecular mechanism of traditional Chinese medicine in preventing cardiotoxicity associated with chemoradiotherapy

“…Myocardial fibrosis is a late manifestation of radiation-induced heart disease (RIHD) ( 50 ). The current studies showed that AS-AM, a DBD-derivative, downregulated TGF-β/Smad and COL-I expression in an X-ray-induced rat CFs fibrotic injury model ( 51 – 53 ) ( Figure 2 ).…”

Research Progress of Traditional Chinese Medicine in Treatment of Myocardial fibrosis

“…Based on the ancient Danggui Buxue Decoction, Angelica sinensis and Hedysarum hedysarum were used as raw materials to prepare the ultrafiltration of Radix Angelica Sinensis and Radix Hedysari (RAS-RH) ( Ma et al, 2019 ). In vivo experiments revealed that RAS-RH (50 mg/kg/day) ( Ma et al, 2020 ) can alleviate the MF induced by X-ray in rats, and its mechanism may be related to the inhibition of microRNA-200a expression by RAS-RH. Xinkang Granule is mainly composed of White Ginseng , Astragalus Membranaceus , Bupleurum , Cimicifuga , Platycodon grandiflorum , Poria cocos, Coix Seed , Zingiber officinale peel , Areca catechuL.…”

Ultrafiltration Extract of Radix Angelica Sinensis and Radix Hedysari Attenuates Risk of Low-Dose X-Ray Radiation-Induced Myocardial Fibrosis In Vitro

“…Moreover, some studies have reported the effect of Radix Hedysari on anti-fibrosis, anti-apoptosis, and anti-oxidation in rat heart [20,21]. Consequently, we complied with original ratio of DBD and prepared ultrafiltration extract of Radix Angelica Sinensis and Radix Hedysari instead of Astragalus membranaceus (RAS-RH) to supplement prophase experiments in vivo model we did [22,23] for further research on radiation-induced heart injury. e crude extract was separated by members of our team using chromatographic methods.…”