Rainbow trout, <i>Salmo gairdnerii</i> Richardson, spermatozoa: effects of cations and pH on motility

Baynes, S. M.; Scott, A. P.; Dawson, Alan P.

doi:10.1111/j.1095-8649.1981.tb05830.x

Cited by 154 publications

(93 citation statements)

References 9 publications

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“…Generally, interactions of ions present in the seminal fluid with the sperm membrane do influence the membrane potential [12] and represent a mechanism of inhibition of spermatozoa in the seminal fluid or sperm duct [8] , allowing the maintenance of the potential of motility before release to the surrounding medium [29] . According to past data, it was revealed that the low K + levels of surrounding medium is key factor for activation of salmonid fish spermatozoa [4,29] , also the increase of semen pH may be responsible for acquisition of motility of spermatozoa in the rainbow trout Oncorhynchus mykiss and chum salmon, Oncorhynchus keta [7,26,27] during passage of spermatozoa from the testis to the spermatic duct i.e., before sperm ejaculation to spawnig medium (water).…”

Section: Discussionmentioning

confidence: 99%

Effects of Stripping Frequency on Semen Quality of Endangered Caspian Brown Trout, Salmo trutta caspius

Hajirezaee¹,

Amiri²,

Mirvaghefi³

2009

American J. of Animal and Veterinary Sciences

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Problem statement: Because of dramatic declines in stocks of endangered Caspian brown trout males, Salmo trutta caspius in Caspian Sea, each male brooder is stripped indispensably more than once during the spawning season in other to artificial insemination in hatchery. The aim of the present study was to assay the changes of indicators of semen quality (sperm motility, sperm production, semen volume and chemical composition of seminal fluid) during these sequential strippings. Approach: The 11 tagged males were stripped four times every 12-14 days with beginning of spermiation period (2 December 2008) towards its end (10 January 2008). One-way Analysis Of Variance (ANOVA) was employed to analyze differences between means of semen parameters. Also, the relationships between semen parameters were tested using the bivariate correlation coefficients of Pearson. Results: The semen volume, sperm density, osmolality and the concentrations of Na + , Cl − , K + , Ca 2+ , Mg 2+ and total protein gradually decreased whereas the values of glucose and triglyceride had no significant changes during sequential strippings. Also, the values of semen pH, the percentage (5s post-activation) and duration of motility were statistically stable until third stripping but a decrease was recorded for these parameters in the fourth stripping. As well as, significant positive correlations were found for sperm density vs. K + , Cl − , Na + , Ca 2+ , Mg 2+ , total protein, spermatocrit; the percentage of motile spermatozoa Vs Ca 2+ , Mg 2+ , K + , Cl − , Na + , total protein and also the duration of motility Vs K + , Cl − , total protein and pH. Conclusion: The semen quality of Caspian brown trout males decrease in successive strippings during spawning season. Also, the knowledge on values and correlations between the sperm motility characteristics and the composition of seminal fluid could be useful to formulation of a species-specific extender solution for cryopreservation of semen of Caspian brown trout.

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Section: Discussionmentioning

confidence: 99%

Effects of Stripping Frequency on Semen Quality of Endangered Caspian Brown Trout, Salmo trutta caspius

Hajirezaee¹,

Amiri²,

Mirvaghefi³

2009

American J. of Animal and Veterinary Sciences

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“…21,22) Trout spermatozoa do not move in the presence of EGTA22) or in the presence of Ca 21-channel blockers. 22,23) Moreover, upon the initiation of movement of sper matozoa, an increase occurs in the intracellular concentra tion of Ca2+ ions in these cells.24,25) These data strongly suggest that external Ca2+ ions might be involved in the in itiation of flagellar movement in salmonid spermatozoa.…”

Section: Discussionmentioning

confidence: 96%

“…Suppression of sperm motility in acidified activating solutions has also been observed for many teleost species. 21,[30][31][32] Redondo-Miiller et al9) reported that milt spermatozoa from carp, with a low potential for movement, acquired motility during 30-minute incubation in K+ rich (150-200 mM KCl) solutions in a pH-independent manner. The minimal level of K + ions for the acquisition of motility was 50mM in a solution of 380 mOsm/kg.…”

Section: Discussionmentioning

confidence: 99%

Ionic Environment Necessary for Maintenance of Potential Motility in the Common Carp Spermatozoa during <i>In Vitro</i> Storage

Ohta¹,

Tsuji

1998

Fisheries science

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“…, and Mg +2 at increased concentrations were antagonistic to K + ions (Scheuring 1925, Baynes et al 1981 but were indispensable to initiate sperm motility (Christen et al 1987, Cosson et al 1989, Billard et al 1995, Krasznai et al 2000, Bondarenko et al 2014 In our studies on brook trout spermatozoa the concentration of ions in the water used for activation was diversifi ed (Table 1 (Billard et al 1995, Lahnsteiner et al 1998, Glogowski et al 2000. It is therefore possible that the increased concentration of K + , Na + , and Ca +2 ions in the water of 10.0‰, though smaller than in the salmonid seminal plasma, does not have to be the reason for the decrease in the values of the studied sperm motility parameters.…”

Section: +2mentioning

confidence: 99%

The effect of water salinity on the motility of spermatozoa of the brook trout, Salvelinus fontinalis (Actinopterygii: Salmoniformes: Salmonidae)

Bonisławska¹

2015

Acta Ichthyol. Piscat.

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Bonisławska M., Szulc J., Formicki K. 2015. The effect of water salinity on the motility of spermatozoa of the brook trout, Salvelinus fontinalis (Actinopterygii: Salmoniformes: Salmonidae). Acta Ichthyol. Piscat. 45 (2): 143-151.Background. Salmonid spermatozoa are characterised by a very short time of activity in the water, therefore sudden water pollution in the form of increased salinity on the spawning grounds may have a negative effect on the sperm motility parameters, thus affecting the subsequent egg fertilisation and, consequently, the number of hatched individuals of the species. The aim of this study was to determine the effect of water salinity on the motility parameters of spermatozoa of brook trout, Salvelinus fontinalis (Mitchill, 1814). Material and methods. Sperm motility was monitored with a camera (Basler A312fc) coupled with Nikon Eclipse 50i light microscope from the moment of their activation (contact with water) until the cessation of movement. The following water-salinity treatments were tested: 1.0‰, 3.0‰, 5.0‰, and 10.0‰. The motility parameters: VCL, VSL, VAP, ALH, BCF, LIN, STR, WOB, and MOT, were analysed with Computer Assisted Sperm Analysis (CASA). Results.The mean values of the studied motility parameters of the brook trout spermatozoa (obtained within 30 s), whose activation took place in the water of 0.35‰ salinity and in water of 1.0‰ and 3.0‰ salinity, did not differ signifi cantly. The highest mean values of motility parameters were recorded for the water of 5.0‰ salinity. The 10.0‰ treatment caused a distinct decrease in the values of all the studied parameters. The percentage of MOT was the highest (37.5%) in the sample activated in the water used for fi sh rearing (0.35‰). In the remaining samples the MOT was lower, and in the water of 10.0‰ salinity it was only 9.1%. No spermatozoa movement of any kind was recorded in the 35th second of the experiment. Conclusion.The values of the motility parameters as well as the percentage of motile spermatozoa (MOT) in the semen decrease with increasing salinity of the water used for activation, and with increasing time of exposure.

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Rainbow trout, Salmo gairdnerii Richardson, spermatozoa: effects of cations and pH on motility

Cited by 154 publications

References 9 publications

Effects of Stripping Frequency on Semen Quality of Endangered Caspian Brown Trout, Salmo trutta caspius

Effects of Stripping Frequency on Semen Quality of Endangered Caspian Brown Trout, Salmo trutta caspius

Ionic Environment Necessary for Maintenance of Potential Motility in the Common Carp Spermatozoa during <i>In Vitro</i> Storage

The effect of water salinity on the motility of spermatozoa of the brook trout, Salvelinus fontinalis (Actinopterygii: Salmoniformes: Salmonidae)

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