Rainbow trout (Oncorhynchus mykiss) gut microbiota is modulated by insect meal from Hermetia illucens prepupae in the diet

Terova, Genciana; Rimoldi, Simona; Ascione, Chiara; Gini, Elisabetta; Ceccotti, Chiara; Gasco, Laura

doi:10.1007/s11160-019-09558-y

Cited by 175 publications

(279 citation statements)

References 89 publications

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“…In agreement with previous findings in rainbow trout (29,58,59) and laying hens (60, 61), we found that the insect meal diet altered the distal intestinal microbiota assemblage resulting in higher microbial richness and diversity. Our findings, showing that the insect meal diet increased the relative abundance of Actinomyces, Bacillus, Brevibacterium, Corynebacterium 1 and Enterococcus, are in accord with recent studies in rainbow trout fed diets containing 30% black soldier fly larvae meal (29,59). Importantly, these results were partly confirmed in other studies employing fluorescence in situ hybridization for targeted profiling of changes in the intestinal microbiota.…”

Section: Sample Origin Effectsupporting

confidence: 93%

“…Our results corroborate previous findings in rainbow trout revealing that mucosa-associated intestinal microbiota was less influenced by dietary inclusion of 30% black soldier fly larvae meal compared to digesta-associated intestinal microbiota (28,29). Results from molecular-based studies on salmonid intestinal microbiota hitherto suggest that diet modulates digesta-and mucosa-associated intestinal microbiota to varying degrees with the latter generally being more resilient to dietary interventions (19,(26)(27)(28)(29)35). As such, current practices of profiling only one of or a mixture of digesta-and mucosa-associated microbiota may obscure the response of intestinal microbiota to dietary changes.…”

Section: Significant Interactions Between Diet and Sample Origin Effectsupporting

confidence: 91%

“…While studies in mammals and fish have revealed substantial differences between the digesta-and mucosa-associated intestinal microbiota (19,(22)(23)(24)(25), most studies investigating diet effects on the intestinal microbiota of fish have sampled the digesta only or a mixture of digesta and mucosa. Evidence is accumulating that digesta-and mucosa-associated intestinal microbiota in fish respond differently to dietary changes (19,(26)(27)(28)(29). Profiling only one of or a mixture of digestaand mucosa-associated microbiota may obscure the response of intestinal microbiota to dietary changes.…”

Section: Introductionmentioning

confidence: 99%

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Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Bruni

Jaramillo-Torres

et al. 2020

Preprint

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Intestinal digesta is commonly used for studying responses of microbiota to dietary shifts, yet evidence is accumulating that it represents an incomplete view of the intestinal microbiota. In a 16-week seawater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially-relevant reference diet or an insect meal diet containing 15% black soldier fly (Hermetia illucens) larvae meal. The digesta-and mucosa-associated distal intestinal microbiota were profiled by 16S rRNA gene sequencing. Regardless of diet, we observed substantial differences between digesta-and mucosa-associated intestinal microbiota. Microbial richness and diversity were much higher in the digesta than the mucosa. The insect meal diet altered the distal intestinal microbiota resulting in higher microbial richness and diversity. The diet effect, however, depended on the sample origin. Digesta-associated intestinal microbiota showed more pronounced changes than the mucosa-associated microbiota. Lastly, multivariate association analyses identified two mucosa-enriched taxa, Brevinema andersonii and unclassified Spirochaetaceae, associated with the expression of genes related to immune responses and barrier function in the distal intestine, respectively. Overall, our data clearly indicate that responses in digesta-and mucosa-associated microbiota to dietary inclusion of insect meal differ, with the latter being more resilient to dietary changes. Microbiota | Gut biogeography | Atlantic salmon | Diet | Black soldier fly Correspondence: yanxianl@nmbu.no Characterizing intestinal microbiota and its associationsLi & Bruni et al. | bioRχiv | May 24, 2020 | 1-8 with host responses is an essential step towards identifying key microbial clades promoting fish health and welfare. Ultimately, a milestone in the fish microbiota research would be knowing how to selectively manipulate the microbiota to improve the growth performance, disease resistance and health status of farmed fish. The main aims of the work presented herein were (i) to compare distal intestinal microbiota of Atlantic salmon fed a commercially relevant diet and an insect meal-based diet, (ii) to further explore the dissimilarity between digesta-and mucosa-associated microbiota and the differences in their response to dietary changes, and (iii) to identify associations between microbial clades and host responses. This work was part of a larger study consisting of a freshwater and seawater feeding trial that aimed to investigate the nutritional value and possible health effects for Atlantic salmon of a protein-rich insect meal produced from black soldier fly (Hermetia illucens) larvae. The present work focuses on the intestinal microbiota in seawater phase Atlantic salmon fed an insect meal diet containing 15% black soldier fly larvae meal for 16 weeks. Results on feed utilization, growth performance, fillet quality, intestinal histomorphology and gene expression have been reported elsewhere (30-32). ResultsHereafter, different sample groups are named based on the combination of die...

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Section: Sample Origin Effectsupporting

confidence: 93%

Section: Significant Interactions Between Diet and Sample Origin Effectsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Bruni

Jaramillo-Torres

et al. 2020

Preprint

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“…Also Mota de Carvalho et al 2019showed that powder of the yellow mealworm has a potential prebiotic effect. This prebiotic effect may be caused by the exoskeleton of insects, chitin (Komi et al 2018), not only in humans (Stull et al 2018) but also in fish (Rimoldi et al 2019;Terova et al 2019). Also insects have the largest repertoire of antimicrobial peptides, which has promoted their development as alternatives to conventional antibiotics, in an attempt to address…”

Section: Nutrition and Healthmentioning

confidence: 99%

Prospects of insects as food and feed

2020

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In the last 10 years, the interest to use insects as food and feed has increased exponentially. In tropical zones, insects are a common food item as they are more readily available as food in nature than in other climate zones. However, if we want to promote insects as food and feed, harvesting from nature is not an option and the farming of these animals is required. This can be done in environmentally controlled facilities. Insects are not only nutritionally excellent food; they may also have health benefits. When using organic side streams as substrate, chemical and biological contaminants need to be considered. People in western countries are not used to eating insects, and therefore, strategies to "convince" consumers of their hygienic safety, environmental sustainability, and tastiness are necessary. The insect sector is maturing fast, but still faces many challenges, which can only be met when all stakeholders cooperate closely. Keywords Edible insects. Insects as food and feed. Circular economy. Nutrition and health. Industrial production. Food safety and legislation. Consumer attitudes Org. Agr.

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“…While studies in mammals and fish have revealed substantial differences between the digesta-and mucosa-associated intestinal microbiota [21,[24][25][26][27], most studies investigating diet effects on the intestinal microbiota of fish have sampled the digesta only or a mixture of digesta and mucosa. Evidence is accumulating that digesta-and mucosa-associated intestinal microbiota in fish respond differently to dietary changes [21,[28][29][30][31]. Profiling only one of or a mixture of digestaand mucosa-associated microbiota may obscure the response of intestinal microbiota to dietary changes.…”

Section: Introductionmentioning

confidence: 99%

Differential response of digesta- and mucosa-associated intestinal microbiota to dietary black soldier fly (Hermetia illucens) larvae meal in seawater phase Atlantic salmon (Salmo salar)

Bruni

Jaramillo-Torres

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Intestinal digesta is commonly used for studying responses of microbiota to dietary shifts, yet evidence is accumulating that it represents an incomplete view of the intestinal microbiota. The present work aims to investigate the differences between digesta- and mucosa-associated intestinal microbiota in Atlantic salmon ( Salmo salar ) and how they may respond differently to dietary perturbations. In a 16-week seawater feeding trial, Atlantic salmon were fed either a commercially-relevant reference diet or an insect meal diet containing ~15% black soldier fly ( Hermetia illucens ) larvae meal. The digesta- and mucosa-associated distal intestinal microbiota were profiled by 16S rRNA gene sequencing. Results: Regardless of diet, we observed substantial differences between digesta- and mucosa-associated intestinal microbiota. Microbial richness and diversity were much higher in the digesta than the mucosa. The insect meal diet altered the distal intestinal microbiota resulting in higher microbial richness and diversity. The diet effect, however, depended on the sample origin. Digesta-associated intestinal microbiota showed more pronounced changes than the mucosa-associated microbiota. Multivariate association analyses identified two mucosa-enriched taxa, Brevinema andersonii and unclassified Spirochaetaceae , associated with the expression of genes related to immune responses and barrier function in the distal intestine, respectively. Conclusions: Our data show that salmon intestinal digesta and mucosa harbor microbial communities with clear differences. Mucosa-associated intestinal microbiota seems more resilient to variations in the diet composition than digesta-associated intestinal microbiota. To fully unveil the response of intestinal microbiota to dietary changes, concurrent profiling of digesta- and mucosa-associated intestinal microbiota is recommended whenever feasible.

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Rainbow trout (Oncorhynchus mykiss) gut microbiota is modulated by insect meal from Hermetia illucens prepupae in the diet

Cited by 175 publications

References 89 publications

Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Differential Response of Digesta- and Mucosa-Associated Intestinal Microbiota to Dietary Black Soldier Fly (Hermetia illucens) Larvae Meal in Seawater Phase Atlantic Salmon (Salmo salar)

Prospects of insects as food and feed

Differential response of digesta- and mucosa-associated intestinal microbiota to dietary black soldier fly (Hermetia illucens) larvae meal in seawater phase Atlantic salmon (Salmo salar)

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