RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction

Davis, Janel L.; Soetikno, Brian T.; Song, Ki Hee; Zhang, Yang; Sun, Cheng

doi:10.1093/bioinformatics/btaa635

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…Remote-sensing image processing technology is utilised in the realm of resources and environmental science to monitor natural catastrophes such as floods, forest fires, and earthquakes, analyse pollution levels, and explore mineral, groundwater, and marine resources [12]. In industry, computer vision allows robots to do components' inspection and automated assembly, as well as other repetitive operations, with great accuracy [13]. Militarily, image processing technology plays an important role in target location, tracking, and weapon guidance.…”

Section: Introductionmentioning

confidence: 99%

An Improved Method Research on Graphics and Image Processing System

2022

Security and Communication Networks

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In the era of high-speed information, with the popularization of network and the rapid development of multimedia communication technology, human society has entered a key new stage of development. Human productivity and living style have been significantly altered as a result of social informatization. The field of computer image processing has also made significant strides recently. Photoshop has evolved into a must-have piece of software for those who work in computer graphics and image processing on a regular basis. It contributes to increased job productivity in the processing of computer visuals and photos because of its extensive functionality, user-friendliness, and humanization. Photoshop is a graphics and image processing technology programme that is very efficient. This software’s image processing function is quite strong, and it is capable of producing graphics and image processing of the highest quality. This technology is widely used in practical work. Professionals are required to operate skillfully and grasp the technology accurately. Photoshop is widely used in current graphics and image processing. While meeting the relevant processing requirements of users, it is also used in various occasions related to image processing.

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Section: Introductionmentioning

confidence: 99%

An Improved Method Research on Graphics and Image Processing System

2022

Security and Communication Networks

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“…For each sample, 32,000 frames were taken with an exposure time of 40 ms to ensure good coverage of binding events in the polymers. Processing of the image stacks by standard single-molecule localization algorithms with Fiji plugins, Thunderstorm, and RainbowStorm, , we obtain a matrix of event data with spatial coordinates, emission photons, spectral wavelength, etc., to allow us to reconstruct the super-resolved images by summing all events (Figure S2). Then, to perform quantitative analysis at a single-fiber level, postprocessing of the data was performed using a previously reported workflow .…”

Section: Resultsmentioning

confidence: 99%

Visualizing the Heterogeneity in Homogeneous Supramolecular Polymers

Archontakis,

Dhiman,

Zhang

et al. 2024

J. Am. Chem. Soc.

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The dynamic properties of supramolecular polymers enable new functionality beyond the limitations of conventional polymers. The mechanism of the monomer exchange between different supramolecular polymers is proposed to be closely associated with local disordered domains within the supramolecular polymers. However, a direct detection of such heterogeneity has never been experimentally probed. Here, we present the direct visualization of the local disordered domains in the backbone of supramolecular polymers by a super-resolution microscopy technique: Nile Red-based spectrally resolved point accumulation for imaging in nanoscale topography (NR-sPAINT). We investigate the local disordered domains in trisamide-based supramolecular polymers comprising a (co)assembly of benzene-1,3,5-tricarboxamide (BTA) and a variant with one of the amide bonds inverted (iBTA). The NR-sPAINT allows us to simultaneously map the spatial distribution and polarity of the local disordered domains along the polymers with a spatial precision down to ∼20 nm. Quantitative autocorrelation and cross-correlation analysis show subtle differences in the spatial distribution of the disordered domains between polymers composed of different variants of BTA monomers. Further, statistical analysis unraveled high heterogeneity in monomer packing at both intra-and interpolymer levels. The results reported here demonstrate the necessity of investigating the structures in soft materials at nanoscale to fully understand their intricacy.

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“…The localization les which contain the single-molecule coordinates (X, Y, T) and the raw single-molecule uorescence spectrum of AF647 and CF680 were obtained via Thunder-STORM and RainbowSTORM for Fiji. 25,26 Additionally, the rst 1500 frames were ltered out to wait for the proper STORM blinking to occur. Furthermore, a density lter (radius 80 nm, neighbors 80) was applied to lter out the nonspecic localizations which occur like sparse points in the eld of view.…”

Section: Sr-dstorm Quanticationmentioning

confidence: 99%