To study the characteristics of nitrogen (N) loss on slopes, different vegetation (bare soil, alfalfa), slopes (5°, 10°, 15°), and rainfall intensities (40, 60, 80 mm/h) were set as variable factors in simulated rainfall experiments. Surface runoff accounts for 60.38–96.16% of total runoff and most N loss (57.69–88.67% of NO3−-N). Alfalfa can reduce average concentrations of N loss in runoff and reduce N loss in surface runoff by more than 48.29%, as well as subsurface runoff by 3.8%. Average N loss in subsurface runoff exceeds that of surface runoff. Rainfall intensity most affects N loss from surface runoff in bare soil conditions, and slope most affects N loss in subsurface runoff. Rainfall intensity in alfalfa treatments most influences runoff volume and N loss. The comprehensive effects of rainfall intensity, slope, and vegetation cover on the total loss of various forms of nitrogen in surface runoff can be described using a linear correlation equation, with a correlation coefficient between 0.84 and 0.91.