Raising Antibodies in Chickens Against Primaquine, Pyrimethamine, Dapsone, Tetracycline, and Doxycycline

Abstract

Antibodies against primaquine, pyrimethamine, dapsone, tetracycline, and doxycycline were raised in chickens inoculated with each drug conjugated to a rabbit albumin carrier. Antibody titres against drug and carrier were highest during week 6 postinoculation. Affinity purified anti-primaquine antibodies did not recognise other drugs, but affinity purified anti-doxycycline and anti-tetracycline antibodies recognised both tetracycline and doxycycline in addition to primaquine. Primaquine was detected in urine from 6 to 12 hours after ingestion of therapeutic doses of the drug by anti-primaquine antibodies in a competitive ELISA. Affinity purified anti-primaquine antibodies detected primaquine in the cytoplasm and localised in organelles in monocytes that had been incubated with therapeutic concentrations of the drug.

The X-ray Crystal Structure of Mannose-binding Lectin-associated Serine Proteinase-3 Reveals the Structural Basis for Enzyme Inactivity Associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) Syndrome

“…Western Blotting and Antibodies-Proteins were resolved by SDS-PAGE, transferred, and immunoblotted using an anti-MASP-3 antibody directed against the unique peptide sequence, NPNVTDQIISSGTRT, which was raised in chickens as previously described (22).…”

New haptens synthesis, antibody production and comparative molecular field analysis for tetracyclines

“…Few attempts have been made to prepare immunogens for TCs in the literature, 17 moreover, most studies have employed rudimentary strategies based on the direct coupling of TC to the carrier protein using homobifunctional aldehydes, carbodiimide (CDI) or diazonium conjugation via Mannich condensation. 20,21 Recently, several authors have synthesized TC derivatives as haptens with active carboxyl or amino groups, such as TC, CTC, OTC and methacycline. 14,15,17,20,22 In these previous reports, the conjugation sites in the TCs or haptens were generally situated in the D ring of the TCs (Fig.…”

Molecular Determinants of the Substrate Specificity of the Complement-initiating Protease, C1r

“…The antibodies used were polyclonal C1r (Abcam), a C1s antibody directed against the unique peptide sequence CSTSVQTSRLAKSKM, and a MASP-3 antibody directed against the unique peptide sequence NPNVTDQIISSGTRT. The latter antibodies were raised in chickens as described previously (12).…”