Ralstonia metallidurans CH34 RpoN sigma factor and the control of nitrogen metabolism and biphenyl utilization
               The GenBank accession number for the sequence determined in this work is AJ131690.

Mouz, Sébastien; Coursange, Evelyne; Toussaint, Ariane

doi:10.1099/00221287-147-7-1947

Cited by 4 publications

(1 citation statement)

References 42 publications

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“…In E. coli, the transcription of atoDA is also regulated by a r 54 dependent activator, but so far no regulator with a clear r 54 signature was found close to the atoDA genes in the genome of CH34. Nevertheless, also the r 54 dependent nitrogen metabolism P-II transcription regulator GlnB (Rmet_0681) (Mouz et al 2001), was found to be overproduced in CH34 in space in the MESSAGE-1 and -2 experiments.…”

Section: Spaceflight Effect On Intracellular and Membrane Protein Conmentioning

confidence: 97%

The response of Cupriavidus metallidurans CH34 to spaceflight in the international space station

Leys

Baatout

Rosier

et al. 2009

Antonie van Leeuwenhoek

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The survival and behavior of Cupriavidus metallidurans strain CH34 were tested in space. In three spaceflight experiments, during three separate visits to the 'International Space Station' (ISS), strain CH34 was grown for 10-12 days at ambient temperature on mineral agar medium. Space- and earth-grown cells were compared post-flight by flow cytometry and using 2D-gel protein analysis. Pre-, in- and post-flight incubation conditions and experiment design had a significant impact on the survival and growth of CH34 in space. In the CH34 cells returning from spaceflight, 16 proteins were identified which were present in higher concentration in cells developed in spaceflight conditions. These proteins were involved in a specific response of CH34 to carbon limitation and oxidative stress, and included an acetone carboxylase subunit, fructose biphosphate aldolase, a DNA protection during starvation protein, chaperone protein, universal stress protein, and alkyl hydroperoxide reductase. The reproducible observation of the over-expression of these same proteins in multiple flight experiments, indicated that the CH34 cells could experience a substrate limitation and oxidative stress in spaceflight where cells and substrates are exposed to lower levels of gravity and higher doses of ionizing radiation. Bacterium C. metallidurans CH34 was able to grow normally under spaceflight conditions with very minor to no effects on cell physiology, but nevertheless specifically altered the expression of a few proteins in response to the environmental changes.

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Section: Spaceflight Effect On Intracellular and Membrane Protein Conmentioning

confidence: 97%

The response of Cupriavidus metallidurans CH34 to spaceflight in the international space station

Leys

Baatout

Rosier

et al. 2009

Antonie van Leeuwenhoek

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Incidence and function of sigma factors in Ralstonia metallidurans and other bacteria

Nies

2004

Archives of Microbiology

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Bacterial sigma factors are essential for directing the bacterial RNA polymerase to promoter regions during transcription initiation. Genomic sequencing of the highly heavy-metal-resistant beta-proteobacterium Ralstonia metallidurans strain CH34 revealed 17 candidate genes for sigma factors. This review compares the sigma factor machinery of R. metallidurans to that of other bacteria. The sigma factors of 105 bacterial genomes were assigned to sigma factor clusters and families formed around the factors from Escherichia coli, Bacillus subtilis, and R. metallidurans. Genes for between 1 and 65 sigma-factor-related proteins were found in these genomes. Although prediction of sigma factor function from sequence comparisons can be misleading, organization of the R. metallidurans sigma factors into clusters and protein families, together with a discussion of the physiological function of members of these clusters, might yield insight into the cellular roles of bacterial sigma factors and the genes that depend on them for their expression.

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Comparative analyses imply that the enigmatic sigma factor 54 is a central controller of the bacterial exterior

et al. 2011

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BackgroundSigma-54 is a central regulator in many pathogenic bacteria and has been linked to a multitude of cellular processes like nitrogen assimilation and important functional traits such as motility, virulence, and biofilm formation. Until now it has remained obscure whether these phenomena and the control by Sigma-54 share an underlying theme.ResultsWe have uncovered the commonality by performing a range of comparative genome analyses. A) The presence of Sigma-54 and its associated activators was determined for all sequenced prokaryotes. We observed a phylum-dependent distribution that is suggestive of an evolutionary relationship between Sigma-54 and lipopolysaccharide and flagellar biosynthesis. B) All Sigma-54 activators were identified and annotated. The relation with phosphotransfer-mediated signaling (TCS and PTS) and the transport and assimilation of carboxylates and nitrogen containing metabolites was substantiated. C) The function annotations, that were represented within the genomic context of all genes encoding Sigma-54, its activators and its promoters, were analyzed for intra-phylum representation and inter-phylum conservation. Promoters were localized using a straightforward scoring strategy that was formulated to identify similar motifs. We found clear highly-represented and conserved genetic associations with genes that concern the transport and biosynthesis of the metabolic intermediates of exopolysaccharides, flagella, lipids, lipopolysaccharides, lipoproteins and peptidoglycan.ConclusionOur analyses directly implicate Sigma-54 as a central player in the control over the processes that involve the physical interaction of an organism with its environment like in the colonization of a host (virulence) or the formation of biofilm.

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Ralstonia metallidurans CH34 RpoN sigma factor and the control of nitrogen metabolism and biphenyl utilization The GenBank accession number for the sequence determined in this work is AJ131690.

Cited by 4 publications

References 42 publications

The response of Cupriavidus metallidurans CH34 to spaceflight in the international space station

The response of Cupriavidus metallidurans CH34 to spaceflight in the international space station

Incidence and function of sigma factors in Ralstonia metallidurans and other bacteria

Comparative analyses imply that the enigmatic sigma factor 54 is a central controller of the bacterial exterior

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