Raman excitation profiles from pigments <i>in vivo</i>

Wagner, W.-D.

doi:10.1002/jrs.1250170111

Cited by 12 publications

(8 citation statements)

References 14 publications

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“…The technique was later used to assess the presence of light-harvesting bacteriochlorophyll and bacteriochlorophyll-protein complexes in purple photosynthetic bacteria (26). In 1986, it was documented that carotenoids can be easily detected in trace amounts in complex material and even within a single living cell on a very short time scale (27). Resonance Raman spectra (RRS) of astaxanthin-protein complexes published soon after showed some details of carotenoid-protein interactions (28).…”

Section: Raman Spectrometry For Pigment Analysis-historical Overviewmentioning

confidence: 99%

Raman Spectroscopy of Microbial Pigments

Jehlička

Edwards

Oren

2014

Appl Environ Microbiol

151

114

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Raman spectroscopy is a rapid nondestructive technique providing spectroscopic and structural information on both organic and inorganic molecular compounds. Extensive applications for the method in the characterization of pigments have been found. Due to the high sensitivity of Raman spectroscopy for the detection of chlorophylls, carotenoids, scytonemin, and a range of other pigments found in the microbial world, it is an excellent technique to monitor the presence of such pigments, both in pure cultures and in environmental samples. Miniaturized portable handheld instruments are available; these instruments can be used to detect pigments in microbiological samples of different types and origins under field conditions.

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Section: Raman Spectrometry For Pigment Analysis-historical Overviewmentioning

confidence: 99%

Raman Spectroscopy of Microbial Pigments

Jehlička

Edwards

Oren

2014

Appl Environ Microbiol

151

114

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“…However, few studies have been undertaken of carotenoids in vivo [7][8][9]. Classical techniques of pigment identification and discrimination are absorption spectroscopy and high-performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

“…The chemical properties of carotenoids originating from biota as well as from sediments in organic solvent extracts are known in depth. However, few studies have been undertaken of carotenoids in vivo [7][8][9]. Classical techniques of pigment identification and discrimination are absorption spectroscopy and high-performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Jehlička

Edwards

Osterrothová

et al. 2014

Phil. Trans. R. Soc. A.

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In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles, in contrast with others which can be considered as biomarkers of other genera. The limits of application of Raman spectroscopy are discussed for a few cases where the current Raman spectroscopic approach does not allow discriminating structurally very similar carotenoids. The database reported can be used for applications in geobiology and exobiology for the detection of pigment signals in natural settings.

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“…seliflavus strain 16 can be assigned to a carotenoid structure (30). Upon closer spectral inspection, the different clustering of E. hirae strain 6 by the Raman approach could be ascribed to the fact that this strain expresses low levels of carotenoid as well.…”

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confidence: 96%

Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study

et al. 2001

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Rapid and accurate identification of enterococci at the species level is an essential task in clinical microbiology since these organisms have emerged as one of the leading causes of nosocomial infections worldwide. Vibrational spectroscopic techniques (infrared [IR] and Raman) could provide potential alternatives to conventional typing methods, because they are fast, easy to perform, and economical. We present a comparative study using phenotypic, genotypic, and vibrational spectroscopic techniques for typing a collection of 18 Enterococcus strains comprising six different species. Classification of the bacteria by Fourier transform (FT)-IR spectroscopy in combination with hierarchical cluster analysis revealed discrepancies for certain strains when compared with results obtained from automated phenotypic systems, such as API and MicroScan. Further diagnostic evaluation using genotypic methods-i.e., PCR of the species-specific ligase and glycopeptide resistance genes, which is limited to the identification of only four Enterococcus species and 16S RNA sequencing, the "gold standard" for identification of enterococci-confirmed the results obtained by the FT-IR classification. These results were later reproduced by three different laboratories, using confocal Raman microspectroscopy, FT-IR attenuated total reflectance spectroscopy, and FT-IR microspectroscopy, demonstrating the discriminative capacity and the reproducibility of the technique. It is concluded that vibrational spectroscopic techniques have great potential as routine methods in clinical microbiology.

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Raman excitation profiles from pigments in vivo

Cited by 12 publications

References 14 publications

Raman Spectroscopy of Microbial Pigments

Raman Spectroscopy of Microbial Pigments

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Classification and Identification of Enterococci: a Comparative Phenotypic, Genotypic, and Vibrational Spectroscopic Study

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