Raman microscopy for cellular investigations — From single cell imaging to drug carrier uptake visualization

Kann, Birthe; Offerhaus, Herman L.; Windbergs, Maike; Otto, Cornelis

doi:10.1016/j.addr.2015.02.006

Cited by 138 publications

(110 citation statements)

References 155 publications

Supporting

Mentioning

108

Contrasting

Unclassified

Order By: Relevance

“…(16,58) In addition to spectral features which may be characteristic of DNA and RNA depletion and The increase in phenylalanine in both nucleolar and nuclear regions seems to be a marker of the late apoptosis stage, at which point nucleolar fragmentation is complete, leaving an empty space with only cellular membrane ( Figure 6). (57,61) Thus, it appears that regression against DOX cellular concentration shows the initial DOX accumulation inside the cells, its nuclear binding and its subsequent effects, including DNA damage, while regression against varying time intervals can elucidate and differentiate both the initial DOX mechanism of action and its cellular effects, such as depletion in DNA, RNA and proteins leading to apoptosis and the cells reactions to those DOX effects which could be a marker of any cellular-drug resistance. The changes in the nucleic acid bands in the nucleolar region are concomitant with the accumulation of DOX, and the changes cease when the accumulation of DOX saturates, associating these with an instantaneous interaction with the biochemical components, and therefore the initial DOX-DNA binding.…”

Section: Amentioning

confidence: 99%

Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines

Farhane¹,

Bonnier

Byrne³

2016

Anal Bioanal Chem

View full text Add to dashboard Cite

Section: Amentioning

confidence: 99%

Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines

Farhane¹,

Bonnier

Byrne³

2016

Anal Bioanal Chem

View full text Add to dashboard Cite

“…A great selection of reviews exist which go into further detail than is presented here for other related areas within SERS. These reviews include general overviews [267][268][269][270][271], and reviews specific to cell studies [272][273][274][275], medical applications [24,25,276,277], and toxicology [278,279].…”

Section: Surface-enhanced Raman Scattering (Sers)mentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

268

189

View full text Add to dashboard Cite

Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

show abstract

“…Additionally, Raman spectroscopy, can be used to monitor the in vitro uptake and distribution of drug carrier systems and the drug itself [47]. Here, we present the approach that comprises full spectral characterization of several chemotherapeutic agents and in vitro subcellular tracking of a known anthracycline compound (DOX) based on its unique spectral features.…”

mentioning

confidence: 99%

Spectroscopic studies of anthracyclines: Structural characterization and in vitro tracking

Szafraniec

Majzner

Farhane³

et al. 2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

View full text Add to dashboard Cite

A broad spectroscopic characterization, using ultraviolet-visible (UV-vis) and Fourier transform infrared absorption as well as Raman scattering, of two commonly used anthracyclines antibiotics (DOX) daunorubicin (DNR), their epimers (EDOX, EDNR) and ten selected analogs is presented. The paper serves as a comprehensive spectral library of UV-vis, IR and Raman spectra of anthracyclines in the solid state and in solution. The particular advantage of Raman spectroscopy for the measurement and analysis of individual antibiotics is demonstrated. Raman spectroscopy can be used to monitor the in vitro uptake and distribution of the drug in cells, using both 488 nm and 785 nm as source wavelengths, with submicrometer spatial resolution, although the cellular accumulation of the drug is different in each case. The high information content of Raman spectra allows studies of the drug-cell interactions, and so the method seems very suitable for monitoring drug uptake and mechanisms of interaction with cellular compartments at the subcellular level.

show abstract

Raman microscopy for cellular investigations — From single cell imaging to drug carrier uptake visualization

Cited by 138 publications

References 155 publications

Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines

Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines

Raman spectroscopy: techniques and applications in the life sciences

Spectroscopic studies of anthracyclines: Structural characterization and in vitro tracking

Contact Info

Product

Resources

About