Raman spectroscopic investigation on the microenvironment of the liver tissues of Zebrafish (Danio rerio) due to titanium dioxide exposure

Palaniappan, Pl. Rm.; Pramod, K.

doi:10.1016/j.vibspec.2011.01.005

Cited by 20 publications

(12 citation statements)

References 45 publications

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“…Previous studies from Federici et al (2007) reported changes in livers from rainbow trout exposed to TiO 2 NPs consisting of condensed nuclear bodies (probably apoptotic bodies) and minor fatty change. In addition, results from FT-Raman techniques on liver microenvironment detected morphological changes in livers from zebrafish exposed to TiO 2 (Palaniappan & Pramod, 2011). Chen et al (2011 a ) exposed zebrafish to TiO 2 NPs (up to 7 mg/L) in a long-term experiment (6 months) demonstrating that TiO 2 NPs-induced toxicity, mortality, and organ histopathology among other alterations.…”

Section: Discussionmentioning

confidence: 99%

Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO₂Nanoparticle Concentrations

et al. 2013

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The toxicity of titanium dioxide nanoparticles (TIO₂ NPs) and oxidative stress effects were studied in two freshwater fish species (Carassius auratus and Danio rerio) exposed for 21 days to different concentrations (0.01, 0.1, 1, 10, 100/mgL) of TiO₂ NPs and to a control (tap water). Additional fish were transferred to clean water for 14 days to assess the ability to recover from exposure to TiO₂ NPs. Activities of the enzyme glutathione-S-transferase (GST) and lipid peroxidation (LPO) (malondialdheyde) were measured as indicators of oxidative stress. Histological and ultra-structural changes in livers from both species of fish were evaluated by light and electron microscopy. Results show a general GST activity increase according to TiO₂ NPs concentrations, which is in agreement with data from LPO. After 21 days, GST activities decreased possibly caused by suppression of GST synthesis as a result of severe stress. Histological and ultra-structural analysis of livers from exposed fish show degeneration of the hepatic tissue and alterations in hepatocytes such as glycogen depletion and an increase in lipofucsin lysosome-like granules. After a depuration period a partial recovery for biochemical markers and cells was observed. The results suggest that TiO₂ promotes alterations in hepatic tissues compatible with oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO₂Nanoparticle Concentrations

et al. 2013

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“…RS is used to investigate the disease development and to test the efficacy of potential medications. Hepatic tissue and cells were studied by RS to research for cancer and hepatitis in different animal models.…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy analysis of lipid droplets content, distribution and saturation level in Non‐Alcoholic Fatty Liver Disease in mice

Kochan

Maślak

Krafft

et al. 2014

Journal of Biophotonics

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Non-Alcoholic Fatty Liver Disease (NAFLD) is a common liver disorder, characterized by an excessive lipids deposition within the hepatic tissue. Due to the lack of clear-cut symptoms and optimal diagnostic method, the actual prevalence of NAFLD and its pathogenesis remains unclear, especially in the early stages of progression. In the presented work confocal Raman microspectroscopy was used to investigate alterations in the chemical composition of the NAFLD-affected liver. We have investigated two NAFLD models, representative for macrovesicular and microvesicular steatosis, induced by High Fat Diet (60 kcal %) and Low Carbohydrate High Protein Diet (LCHP), respectively. In both models we confirmed the development of NAFLD, manifested by the presence of lipid droplets (LDs), but of different sizes. Model of macrovesicular steatosis was characterized by large LDs, whereas in the microvesicular steatosis model small droplets were found. In both models, however, we observed a significant decrease in the degree of unsaturation of lipids, in comparison to the control. In addition, for both models, the impact of medical treatment with selected drugs (perindopril and nicotinic acid, respectively) was tested, indicating a significant influence of medicine not only on the occurrence and size of the droplets, but also on their composition. In both cases the drug treatment resulted in an increase of the degree of unsaturation of lipids forming droplets. Confocal Raman microspectroscopy was proven to be a powerful tool providing detailed insight into selected areas of hepatic tissue, following the NAFLD pathogenesis and diagnostic potential of the applied drugs.

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“…However, the differences are that TiO 2 NPs have strong UV shielding effect, good dispersion, and good weather resistance and are more expensive compared to food‐grade titanium dioxide. Generally, it was accepted that TiO 2 NPs are biologically inactive in experimental animals and humans, but recent researches suggested that long‐term exposure to TiO 2 NPs might have negative biological effects in mice . Studies have unequivocally showed that TiO 2 NP exposure can induce injury in the spleen, brain, heart, liver, kidney, and lung of mice.…”

Section: Introductionmentioning

confidence: 99%

Lung inflammation caused by long‐term exposure to titanium dioxide in mice involving in NF‐κB signaling pathway

Liu

Hong

Zhou

et al. 2016

J Biomedical Materials Res

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Titanium dioxide nanoparticles (TiO NPs) are used in many fields, such as paints, medicine additives, food additives, sunscreens, and agriculture. The aim of this study was to investigate the mechanism behind the formation of inflammation induced by TiO NPs. ICR mice were exposed to TiO NPs through intragastric administration at 2.5, 5, and 10 mg/kg body weight every day for 90 consecutive days. The experiment suggested that long-term exposure to TiO NPs resulted in an obvious inflammatory response in mice lung tissues, which led to a thickened alveoli septum, lung hyperemia, and titanium accumulation. Furthermore, our results show that TiO NPs exposure remarkably altered the expression of inflammation-related cytokines, with increases in proinflammatory cytokines-such as nucleic factor-κB, interferon-α, interferon-β, interleukin-1β, interleukin-6, cyclo-oxygen-ase, interleukin-8, interferon-inducible protein-10, and platelet-derived growth factor AB-and decreases in anti-inflammatory cytokines-such as inhibitor of NF-κB suppressor of cytokine signaling 1, endothelin 1, peroxisome proliferators-activated receptors-γ, and peroxisome proliferators-activated receptors coactivator-1α. This finding indicated that TiO NPs cause lung inflammation in mice after intragastric administration, primarily through the NF-κB signaling pathways. Therefore, more attention should be placed on the application of TiO NPs and their potential long-term effects, especially in human beings. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 720-727, 2017.

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Raman spectroscopic investigation on the microenvironment of the liver tissues of Zebrafish (Danio rerio) due to titanium dioxide exposure

Cited by 20 publications

References 45 publications

Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO₂Nanoparticle Concentrations

Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO₂Nanoparticle Concentrations

Raman spectroscopy analysis of lipid droplets content, distribution and saturation level in Non‐Alcoholic Fatty Liver Disease in mice

Lung inflammation caused by long‐term exposure to titanium dioxide in mice involving in NF‐κB signaling pathway

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Raman spectroscopic investigation on the microenvironment of the liver tissues of Zebrafish (Danio rerio) due to titanium dioxide exposure

Cited by 20 publications

References 45 publications

Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO2Nanoparticle Concentrations

Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO2Nanoparticle Concentrations

Raman spectroscopy analysis of lipid droplets content, distribution and saturation level in Non‐Alcoholic Fatty Liver Disease in mice

Lung inflammation caused by long‐term exposure to titanium dioxide in mice involving in NF‐κB signaling pathway

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Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO₂Nanoparticle Concentrations

Liver Alterations in Two Freshwater Fish Species (Carassius auratusandDanio rerio) Following Exposure to Different TiO₂Nanoparticle Concentrations