Raman spectroscopy for monitoring carotenoids in processed Bunchosia glandulifera pulps

Carvalho, Dermeval de; Sebben, Juliano Antônio; Moura, Neusa Fernandes de; Trierweiler, Jorge Otávio; Espíndola, Juliana da Silveira

doi:10.1016/j.foodchem.2019.04.120

Cited by 16 publications

(4 citation statements)

References 27 publications

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“…For Raman measurements, when excited with 532 and 633 nm lasers, the single-cell Raman spectra of AAPB and non-AAPB strains were very similar by showing three prominent Raman peaks at approximately 1000, 1157, and 1517 cm –1 (Figures and S2). These bands were assigned to carotenoids that have been widely detected in photosynthetic and CO 2 -fixation microorganisms, , consistent with UV–vis absorption observations. Such strong carotenoid bands were due to resonance Raman enhancement of carotenoids because the laser wavelengths of 532 and 633 nm were within or close to the electronic absorption of carotenoids.…”

Section: Resultsmentioning

confidence: 99%

Aerobic Anoxygenic Phototrophic Bacteria in the Marine Environments Revealed by Raman/Fluorescence-Guided Single-Cell Sorting and Targeted Metagenomics

Xu,

Yue,

et al. 2024

Environ. Sci. Technol.

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Aerobic anoxygenic phototrophic bacteria (AAPB) contribute profoundly to the global carbon cycle. However, most AAPB in marine environments are uncultured and at low abundance, hampering the recognition of their functions and molecular mechanisms. In this study, we developed a new cultureindependent method to identify and sort AAPB using single-cell Raman/fluorescence spectroscopy. Characteristic Raman and fluorescent bands specific to bacteriochlorophyll a (Bchl a) in AAPB were determined by comparing multiple known AAPB with non-AAPB isolates. Using these spectroscopic biomarkers, AAPB in coastal seawater, pelagic seawater, and hydrothermal sediment samples were screened, sorted, and sequenced. 16S rRNA gene analysis and functional gene annotations of sorted cells revealed novel AAPB members and functional genes, including one species belonging to the genus Sphingomonas, two genera affiliated to classes Betaproteobacteria and Gammaproteobacteria, and function genes bchCDIX, pucC2, and puf L related to Bchl a biosynthesis and photosynthetic reaction center assembly. Metagenomeassembled genomes (MAGs) of sorted cells from pelagic seawater and deep-sea hydrothermal sediment belonged to Erythrobacter sanguineus that was considered as an AAPB and genus Sphingomonas, respectively. Moreover, multiple photosynthesis-related genes were annotated in both MAGs, and comparative genomic analysis revealed several exclusive genes involved in amino acid and inorganic ion metabolism and transport. This study employed a new single-cell spectroscopy method to detect AAPB, not only broadening the taxonomic and genetic contents of AAPB in marine environments but also revealing their genetic mechanisms at the single-genomic level.

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Section: Resultsmentioning

confidence: 99%

Aerobic Anoxygenic Phototrophic Bacteria in the Marine Environments Revealed by Raman/Fluorescence-Guided Single-Cell Sorting and Targeted Metagenomics

Xu,

Yue,

et al. 2024

Environ. Sci. Technol.

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“…Interest has recently been growing in Raman spectroscopy, a fast, nondestructive, eco-friendly, and highly sensitivity analytical technique for the identification and quantification of food microconstituents [76,77]. Foods may therefore be classified with Raman spectroscopic information in combination with chemometric tools [77].…”

Section: Raman Spectroscopymentioning

confidence: 99%

Modern Analytical Techniques for Berry Authentication

Carrillo,

Tomasevic,

Barba

et al. 2023

Chemosensors

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The health-related properties attributed to berries and the subsequent interest awakened within the market of functional foods mean that these small fruits may be potential targets for food fraud. In this review, studies on berry authentication through modern analytical techniques are discussed in detail. Most of the studies reported to date are related to chemical approaches, mainly chromatographic techniques. Other chemical (NMR, NIR, and Raman spectroscopy), biomolecular, and isotopic methods have also delivered promising results in the field of berry authentication, although there is still limited information available in this respect. Despite the potential of the methods described in the present review, to date, there is no universal one. Therefore, combinations of different approaches in order to complement each other are increasingly used (e.g., HPTLC and mass spectrometry; Raman and IR spectroscopies; biomolecular and analytical techniques…). Considering that adulteration practices are increasingly evolving, continuous research in the field of food authentication is needed, especially in the case of berries, since there are still some berry species that have not yet been included in any authentication study.

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“…The content of CAROT was quantified using Raman spectroscopy in processed Bunchosia glandulifera, a fruit that originated from Brazil. The authors used PCA and PLS regression to identify and quantify total CAROT in B. glandulifera ( Carvalho et al, 2019 ).…”

Section: Measurement Of Metabolites In Fruitsmentioning

confidence: 99%

Insights on the role of chemometrics and vibrational spectroscopy in fruit metabolite analysis

Sultanbawa

Smyth

Truong

et al. 2021

Food Chemistry: Molecular Sciences

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Raman spectroscopy for monitoring carotenoids in processed Bunchosia glandulifera pulps

Cited by 16 publications

References 27 publications

Aerobic Anoxygenic Phototrophic Bacteria in the Marine Environments Revealed by Raman/Fluorescence-Guided Single-Cell Sorting and Targeted Metagenomics

Aerobic Anoxygenic Phototrophic Bacteria in the Marine Environments Revealed by Raman/Fluorescence-Guided Single-Cell Sorting and Targeted Metagenomics

Modern Analytical Techniques for Berry Authentication

Insights on the role of chemometrics and vibrational spectroscopy in fruit metabolite analysis

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