Raman Spectroscopy for Noninvasive Monitoring of Umbilical Cord Mesenchymal Stem Cells Viability Transitions

P.Chen,; F.Zhang,; Lin, Li‐Min; Bai, Hua; L.Zhang,; Tang, Guiqian; Fang, Hui; Mu, Guoguang; Gong, Wei; Liu, Z. P.; Han, Zhi Bo; Zhao, Hui; Han, Zhong Chao

doi:10.5772/24103

Cited by 6 publications

(4 citation statements)

References 45 publications

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“…Furthermore, the complex vibrational response of proteins in the 1200–1350 cm –1 range makes the interpretation of the amide III band very difficult. However, previous studies shed some light on our findings . The reduction of the amide III α-helix and the elevation of the amide III β-sheet in adenoviral conjunctivitis-infected tear biofluids indicates morphological changes in the secondary structures of proteins.…”

Section: Resultsmentioning

confidence: 53%

“…However, previous studies shed some light on our findings. 41 The reduction of the amide III α-helix and the elevation of the amide III β-sheet in adenoviral conjunctivitis-infected tear biofluids indicates morphological changes in the secondary structures of proteins. This change involves interactions between intercellular reactive oxygen species (ROS) and biological macromolecules.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Label-Free Biochemical Analytic Method for the Early Detection of Adenoviral Conjunctivitis Using Human Tear Biofluids

et al. 2014

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Cell culture and polymerase chain reaction are currently regarded as the gold standard for adenoviral conjunctivitis diagnosis. They maximize sensitivity and specificity but require several days to 3 weeks to get the results. The aim of this study is to determine the potential of Raman spectroscopy as a stand-alone analytical tool for clinical diagnosis of adenoviral conjunctivitis using human tear fluids. A drop-coating deposition surface enhanced Raman scattering (DCD-SERS) method was identified as the most effective method of proteomic analysis in tear biofluids. The proposed DCD-SERS method (using a 2-μL sample) led to Raman spectra with high reproducibility, noise-independence, and uniformity. Additionally, the spectra were independent of the volume of biofluids used and detection zones, including the ring, middle, and central zone, with the exception of the outer layer of the ring zone. Assessments with an intensity ratio of 1242-1342 cm(-1) achieved 100% sensitivity and 100% specificity in the central zone. Principal component analysis assessments achieved 0.9453 in the area under the receiver operating characteristic curve (AUC) as well as 93.3% sensitivity and 94.5% specificity in the central zone. Multi-Gaussian peak assessments showed that the differences between these two groups resulted from the reduction of the amide III α-helix structures of the proteins. The presence of adenovirus in tear fluids could be detected more accurately in the center of the sample than in the periphery. The DCD-SERS technique allowed for high chemical structure sensitivity without additional tagging or chemical modification, making it a good alternative for early clinical diagnosis of adenoviral conjunctivitis. Therefore, we are hopeful that the DCD-SERS method will be approved for use in ophthalmological clinics in the near future.

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Section: Resultsmentioning

confidence: 53%

Section: ■ Results and Discussionmentioning

confidence: 99%

Label-Free Biochemical Analytic Method for the Early Detection of Adenoviral Conjunctivitis Using Human Tear Biofluids

et al. 2014

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“…In the study of Chen et al [55], Raman microspectroscopy was applied to human umbical cord blood mesenchymal stem cells (hUC-MSCs) in different cellular states in order to find sensitive molecular vibrations that can be used to determine viability of hUC-MSCs. The intensity decrease of the band at 1342 cm −1 which is attributed from C-H deformation in proteins reflects degradation of proteins.…”

Section: Mesenchymal Stem Cells Applicationsmentioning

confidence: 99%

Role of Vibrational Spectroscopy in Stem Cell Research

Aksoy¹,

Severcan

2012

Spectroscopy: An International Journal

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Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the stem cells have some unwanted effects on the properties of stem cells, and these methods also do not provide real-time information about cellular conditions. These challenges enforce the usage of nondestructive, rapid, sensitive, high quality, label-free, cheep, and innovative chemical monitoring methods. In this context, vibrational spectroscopy provides promissing alternative to get new information into the field of stem cell biology for chemical analysis, quantification, and imaging of stem cells. Raman and infrared spectroscopy and imaging can be used as a new complimentary spectroscopic approaches to gain new insight into stem cell reseaches for future therapeutic and regenerative medicines. In this paper, recent developments in applications of vibrational spectroscopy techniques for stem cell characterization and identification are presented.

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“…Complementary to FLIM imaging, non-invasive Raman microspectroscopy (RMS) enables the marker-independent and molecular-sensitive identification and localization of subcellular structures. Molecular fingerprints of Raman active biomolecules such as proteins, lipids, or nucleic acids can reflect a specific tissue state or cellular phenotype [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Data-Driven Identification of Biomarkers for In Situ Monitoring of Drug Treatment in Bladder Cancer Organoids

Becker

Fischer

Fleck

et al. 2022

IJMS

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Three-dimensional (3D) organoid culture recapitulating patient-specific histopathological and molecular diversity offers great promise for precision medicine in cancer. In this study, we established label-free imaging procedures, including Raman microspectroscopy (RMS) and fluorescence lifetime imaging microscopy (FLIM), for in situ cellular analysis and metabolic monitoring of drug treatment efficacy. Primary tumor and urine specimens were utilized to generate bladder cancer organoids, which were further treated with various concentrations of pharmaceutical agents relevant for the treatment of bladder cancer (i.e., cisplatin, venetoclax). Direct cellular response upon drug treatment was monitored by RMS. Raman spectra of treated and untreated bladder cancer organoids were compared using multivariate data analysis to monitor the impact of drugs on subcellular structures such as nuclei and mitochondria based on shifts and intensity changes of specific molecular vibrations. The effects of different drugs on cell metabolism were assessed by the local autofluorophore environment of NADH and FAD, determined by multiexponential fitting of lifetime decays. Data-driven neural network and data validation analyses (k-means clustering) were performed to retrieve additional and non-biased biomarkers for the classification of drug-specific responsiveness. Together, FLIM and RMS allowed for non-invasive and molecular-sensitive monitoring of tumor-drug interactions, providing the potential to determine and optimize patient-specific treatment efficacy.

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Raman Spectroscopy for Noninvasive Monitoring of Umbilical Cord Mesenchymal Stem Cells Viability Transitions

Cited by 6 publications

References 45 publications

Label-Free Biochemical Analytic Method for the Early Detection of Adenoviral Conjunctivitis Using Human Tear Biofluids

Label-Free Biochemical Analytic Method for the Early Detection of Adenoviral Conjunctivitis Using Human Tear Biofluids

Role of Vibrational Spectroscopy in Stem Cell Research

Data-Driven Identification of Biomarkers for In Situ Monitoring of Drug Treatment in Bladder Cancer Organoids

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