Raman spectroscopy in chemical bioanalysis

Baena, Josefa R.; Lendl, Bernhard

doi:10.1016/j.cbpa.2004.08.014

Cited by 146 publications

(99 citation statements)

References 41 publications

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“…4,35,38,42,55 There are 3 optical spectroscopy types that are used frequently in biomedicine to monitor light-tissue interaction and therefore to perform in vivo tissue characterization: diffuse reflectance spectroscopy, 6,7,9,12,13,19,20,25,30,34,39,48,50,54,[56][57][58] fluorescence spectroscopy, 26,27 and Raman spectroscopy. 2,11,15,16,31,33 Each of these spectroscopy types targets a particular type of light-tissue interaction and a subset of biological molecules (Fig. 1).…”

Section: Optical Spectroscopymentioning

confidence: 99%

The role of optical spectroscopy in epilepsy surgery in children

Bhatia

Ragheb

Johnson

et al. 2008

FOC

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Object Surgery is an important therapeutic modality for pediatric patients with intractable epilepsy. However, existing imaging and diagnostic technologies such as MR imaging and electrocochleography (ECoG) do not always effectively delineate the true resection margin of an epileptic cortical lesion because of limitations in their sensitivity. Optical spectroscopic techniques such as fluorescence and diffuse reflectance spectroscopy provide a nondestructive means of gauging the physiological features of the brain in vivo, including hemodynamics and metabolism. In this study, the authors investigate the feasibility of using combined fluorescence and diffuse reflectance spectroscopy to assist epilepsy surgery in children. Methods In vivo static fluorescence and diffuse reflectance spectra were acquired from the brain in children undergoing epilepsy surgery. Spectral measurements were obtained using a portable spectroscopic system in conjunction with a fiber optic probe. The optical investigations were conducted at the normal and abnormal cortex as defined by intraoperative ECoG and preoperative imaging studies. Biopsy samples were taken from the investigated sites located within the zone of resection. The optical spectra were classified into multiple subsets in accordance with the ECoG and histological study results. The authors used statistical comparisons between 2 given data subsets to identify unique spectral features. Empirical discrimination algorithms were developed using the identified spectral features to determine if the objective of the study was achieved. Results Fifteen pediatric patients were enrolled in this pilot study. Elevated diffuse reflectance signals between 500 and 600 nm and/or between 650 and 850 nm were observed commonly in the investigated sites with abnormal ECoG and/or histological features in 10 patients. The appearance of a fluorescent peak at 400 nm was observed in both normal and abnormal cortex of 5 patients. These spectral alterations were attributed to changes in morphological and/or biochemical characteristics of the epileptic cortex. The sensitivities and specificities of the empirical discrimination algorithms, which were constructed using the identified spectral features, were all > 90%. Conclusions The results of this study demonstrate the feasibility of using static fluorescence and diffuse reflectance spectroscopy to differentiate normal from abnormal cortex on the basis of intraoperative assessment of ECoG and histological features. It is therefore possible to use fluorescence and diffuse reflectance spectroscopy as an aid in epilepsy surgery.

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Section: Optical Spectroscopymentioning

confidence: 99%

The role of optical spectroscopy in epilepsy surgery in children

Bhatia

Ragheb

Johnson

et al. 2008

FOC

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“…These molecular constituents are highly abundant molecules in a cell. Raman spectroscopy methods have gained increased attention for biological purposes due to their high sensitivity to subtle molecular changes, and their ability to probe without the use of added contrast agents of the techniques (45). The Raman spectra of each molecule are uniquely based upon the molecular composition of their molecular bonds, which suggest that Raman based imaging should discriminate between distinct molecules.…”

Section: The Future Of Cars In Biological Imagingmentioning

confidence: 99%

Coherent anti‐stokes Raman scattering microscopy: A biological review

2006

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Microscopic imaging of cells and tissues are generated by the interaction of light with either the sample itself or contrast agents that label the sample. Most contrast agents, however, alter the cell in order to introduce molecular labels, complicating live cell imaging. The interaction of light from multiple laser sources has given rise to microscopy, based on Raman scattering or vibrational resonance, which demonstrates selectivity to specific chemical bonds while imaging unmodified live cells. Here, we discuss the nonlinear optical technique of coherent anti‐Stokes Raman scattering (CARS) microscopy, its instrumentation, and its status in live cell imaging. © 2006 International Society for Analytical Cytology

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“…These emerging or validated optical imaging techniques include Raman spectroscopy imaging for highly detailed chemical information of tissue sample, spectral reflectance imaging for identifying some tissue/cell abnormal variations (e.g., cancer), 2-photon and other confocal microimaging techniques for ultrathin tissue (virtual) sectioning and 3D microimaging (Baena and Lendl, 2004;Rubart, 2004;Singer et al, 2005;Chung et al, 2006;Pawley, 2006). As demonstrated in Figure 6, 2-photon/single-photon confocal immunofluorescence microscopy was applied on a mouse brain tissue slide Vol.…”

Section: Quantitative Analysis and Computer-assisted Image Datamentioning

confidence: 99%

Modern Imaging Technologies in Toxicologic Pathology: An Overview

Ying

Monticello

2006

Toxicol Pathol

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Modern imaging technology, now utilized in most biomedical research areas (bioimaging), enables the detection and visualization of biological processes at various levels of the molecule, organelle, cell, tissue, organ and/or whole body. In toxicologic pathology, the impact of modern imaging technology is becoming apparent from digital histopathology to novel molecular imaging for in vivo studies. This overview summarizes recent progresses in digital microscopy imaging and newly developed digital slide techniques. Applications of virtual microscopy imaging are discussed and compared to traditional optical microscopy reading. New generation digital pathology approaches, including automatic slide inspection, digital slide databases and image management are briefly introduced. Commonly used in vivo preclinical imaging technologies are also summarized. While most of these new imaging techniques are still undergoing rapid development, it is important that toxicologic pathologists embrace and utilize these technologies as advances occur.

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Raman spectroscopy in chemical bioanalysis

Cited by 146 publications

References 41 publications

The role of optical spectroscopy in epilepsy surgery in children

The role of optical spectroscopy in epilepsy surgery in children

Coherent anti‐stokes Raman scattering microscopy: A biological review

Modern Imaging Technologies in Toxicologic Pathology: An Overview

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