Raman spectroscopy of yeast cells cultured on a deuterated substrate

Omelchenko, A.N.; Okotrub, Konstantin A.; Surovtsev, N. V.

doi:10.1016/j.saa.2023.123262

Cited by 5 publications

(10 citation statements)

References 40 publications

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“…Previously reported studies have reported that the substitution of H with D affects the hydrogen bond, disruption of OH/D group orientation and change in symmetry, lipid–protein interactions, protein denaturation process, and lipid bilayer. 22 , 30 − 32 At these concentrations, the stationary phase was found to be reached earlier, suggesting that a higher D 2 O abundance has a negative effect on the growth of the microbial cells.…”

Section: Resultsmentioning

confidence: 91%

“…The next analysis was to examine whether the D 2 O incorporation also induces spectral changes in the biofingerprint region, which can be utilized as a metabolic marker to differentiate among different modes of antibiotic action. A weak peak observed at 989 cm –1 appears due to the deuteration of the microbial phenylalanine aromatic ring. ,, Another interpretation of origin of this band is due to the deuteration of scissoring CH 2 leading to the formation of CD 2 . As the deuterium concentration is at 40%, complete redshift of this band was not observed.…”

Section: Resultsmentioning

confidence: 96%

“…It has been well established in previously reported studies that the C–D band acts as a prominent Raman metabolic activity spectral marker for cells. ,− For validating the feasibility of the growth of E. coli in D 2 O-labeled culture medium, microbial cells were grown in different concentrations at 0%, 1%, 5%, 10%, 20%, 40%, 50%, and 100%.…”

Section: Resultsmentioning

confidence: 99%

“…It has been well established in previously reported studies that the C–D band acts as a prominent Raman metabolic activity spectral marker for cells. 22 , 28 − 30 For validating the feasibility of the growth of E. coli in D 2 O-labeled culture medium, microbial cells were grown in different concentrations at 0%, 1%, 5%, 10%, 20%, 40%, 50%, and 100%. Postinoculation, optical density measurements were performed at different time points; 0, 2, 4, 6, 8, 12, and 24 h. The growth curve is shown in Figure 1 A.…”

Section: Resultsmentioning

confidence: 99%

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Sensing the Bactericidal and Bacteriostatic Antimicrobial Mode of Action Using Raman Deuterium Stable Isotope Probing (DSIP) in Escherichia coli

Karlo,

Vijay,

Phaneeswar

et al. 2024

ACS Omega

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The mode of action of antibiotics can be broadly classified as bacteriostatic and bactericidal. The bacteriostatic mode leads to the arrested growth of the cells, while the bacteriocidal mode causes cell death. In this work, we report the applicability of deuterium stable isotope probing (DSIP) in combination with Raman spectroscopy (Raman DSIP) for discriminating the mode of action of antibiotics at the community level. Escherichia coli, a well-known model microbe, was used as an organism for the study. We optimized the concentration of deuterium oxide required for metabolic activity monitoring without compromising the microbial growth. Our findings suggest that changes in the intensity of the C–D band in the high-wavenumber region could serve as a quantifiable marker for determining the antibiotic mode of action. This can be used for early identification of the antibiotic’s mode of action. Our results explore the new perspective that supports the utility of deuterium-based vibrational tags in the field of clinical spectroscopy. Understanding the antibiotic’s mode of action on bacterial cells in a short and objective manner can significantly enhance the clinical management abilities of infectious diseases and may also help in personalized antimicrobial therapy.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Sensing the Bactericidal and Bacteriostatic Antimicrobial Mode of Action Using Raman Deuterium Stable Isotope Probing (DSIP) in Escherichia coli

Karlo,

Vijay,

Phaneeswar

et al. 2024

ACS Omega

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“…20,22,28 Another interpretation of this band at 989 cm -1 is to the deuteration of scissoring CH2 to leading to the formation of CD2. 28 As the deuterium concentration is taken at 40% complete red shift of this band is not observed. This leads to a visible but weak signal at 989 cm -1 which can act as possible qualitative marker band for monitoring deuteration level and corresponding metabolic activity.…”

Section: Raman Dsip In the Biofingerprint Region To Unravel The Diffe...mentioning

confidence: 99%

Sensing the bactericidal and bacteriostatic antimicrobial mode of action using Raman-Deuterium stable isotope probing (DSIP)

Karlo,

Vijay,

Phaneeswar

et al. 2024

Preprint

View full text Add to dashboard Cite

The mode of actions of antibiotics can be broadly classified as bacteriostatic and bactericidal. The bacteriostatic mode leads to the arrested growth of the cells while the bactericidal mode causes cell death. In this work, we report the applicability of Deuterium stable isotope probing (DSIP) in combination with Raman spectroscopy (Raman DSIP) for discrimination among antibiotics on the basis of their mode of action at community level. We optimized the concentration of deuterium oxide required for metabolic activity monitoring without compromising the microbial growth. We also identified a novel carbon-deuterium Raman metabolic qualitative spectral marker in the biofingerprint region. This can be used for early identification of the antibiotic’s mode of action. Our results explores the new perspective which supports the utility of Deuterium based vibrational tags in the field of clinical spectroscopy. Understanding the antibiotic’s mode of action on bacterial cells in a short and objective manner can significantly enhance the clinical management abilities of infectious diseases and may also help in personalised antimicrobial therapy.Abstract Figure

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Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica

Briel,

Feuser,

Moldenhauer

et al. 2025

Journal of Biotechnology

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Raman spectroscopy of yeast cells cultured on a deuterated substrate

Cited by 5 publications

References 40 publications

Sensing the Bactericidal and Bacteriostatic Antimicrobial Mode of Action Using Raman Deuterium Stable Isotope Probing (DSIP) in Escherichia coli

Sensing the Bactericidal and Bacteriostatic Antimicrobial Mode of Action Using Raman Deuterium Stable Isotope Probing (DSIP) in Escherichia coli

Sensing the bactericidal and bacteriostatic antimicrobial mode of action using Raman-Deuterium stable isotope probing (DSIP)

Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica

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