Raman Spectroscopy Techniques for Skin Cancer Detection and Diagnosis

Bratchenko, Ivan А.; Artemyev, Dmitry N.; Khristoforova, Yulia А.; Bratchenko, Lyudmila A.; Myakinin, Oleg O.; Moryatov, Alexander A.; Orlov, Andrey E.; Kozlov, Sergey V.; Zakharov, Valery P.

doi:10.1007/978-3-030-44594-2_10

Cited by 3 publications

(1 citation statement)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Raman microscopy provides a chemically sensitive, label-free technique for imaging lycopene in cells. Raman microscopy has the potential to monitor cellular localization in drug delivery , and has previously been used to image carotenoids in cells. , Resonance Raman spectroscopy has been utilized for the determination of lycopene isomerization, amount of lycopene in living tissue, − and tomato lycopene content . The Raman signal of lycopene is therefore well established.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Imaging of Lycopene Delivery to Prostate Cancer Cells

et al. 2022

View full text Add to dashboard Cite

The ability to monitor the uptake and distribution of food nutrients in in vitro cell culture models is key to understanding the efficacy of these nutraceuticals to treat and prevent disease. Lycopene is a carotenoid found in chloroplasts and chromoplasts of tomatoes, providing the familiar red color, and a bioactive that inhibits prostate carcinogenesis. We employed live-cell Raman microscopy to visualize lycopene delivery from tween 80 micelles into PC-3 prostate cancer cells. The tween 80 micelle provides a mimic of natural lipoprotein complexes that deliver lycopene in vivo, overcomes the low aqueous solubility of lycopene and challenges replicating physiological uptake to cells, and provides a stable signal to assess cellular uptake of the nutraceutical formulation. The Raman images indicate subcellular localization of the lycopene within the cells. The lycopene Raman signal is resonantly enhanced at an excitation wavelength of 532 nm, providing a convenient, sensitive, and label-free technique to detect and quantify lycopene uptake in living cells. Analysis of the acquired Raman spectra in the maps determines the concentration of lycopene at each point in the cell. In addition to the expected lycopene Raman signal, Raman scattering from the tween 80 vehicle is also mapped in the cells. The Raman data correlates with scattering features observed in darkfield microscopy images of the cells, which display the cell membrane and other features for reference. Overall, the Raman maps indicate lycopene likely accumulates in lipid membranes of cytoplasmic organelles.

show abstract

Section: Introductionmentioning

confidence: 99%

In Vitro Imaging of Lycopene Delivery to Prostate Cancer Cells

et al. 2022

View full text Add to dashboard Cite

show abstract

A Method for Fluorescent Diagnosis of Malignant Cutaneous Neoplasms Using Ytterbium Phthalocyanines

Stavtsev

Dubinina²,

Gorbunova³

et al. 2022

Biomed Eng

View full text Add to dashboard Cite

Cutaneous Oncology: Strategies for Melanoma Prevention, Diagnosis, and Therapy

Saeed,

Shahbaz,

Maqsood

et al. 2024

Cancer Control

View full text Add to dashboard Cite

Skin cancer comprises one-third of all diagnosed cancer cases and remains a major health concern. Genetic and environmental parameters serve as the two main risk factors associated with the development of skin cancer, with ultraviolet radiation being the most common environmental risk factor. Studies have also found fair complexion, arsenic toxicity, indoor tanning, and family history among the prevailing causes of skin cancer. Prevention and early diagnosis play a crucial role in reducing the frequency and ensuring effective management of skin cancer. Recent studies have focused on exploring minimally invasive or non-invasive diagnostic technologies along with artificial intelligence to facilitate rapid and accurate diagnosis. The treatment of skin cancer ranges from traditional surgical excision to various advanced methods such as phototherapy, radiotherapy, immunotherapy, targeted therapy, and combination therapy. Recent studies have focused on immunotherapy, with the introduction of new checkpoint inhibitors and personalized immunotherapy enhancing treatment efficacy. Advancements in multi-omics, nanotechnology, and artificial intelligence have further deepened the understanding of the mechanisms underlying tumoral growth and their interaction with therapeutic effects, which has paved the way for precision oncology. This review aims to highlight the recent advancements in the understanding and management of skin cancer, and provide an overview of existing and emerging diagnostic, prognostic, and therapeutic modalities, while highlighting areas that require further research to bridge the existing knowledge gaps.

show abstract

Raman Spectroscopy Techniques for Skin Cancer Detection and Diagnosis

Cited by 3 publications

References 98 publications

In Vitro Imaging of Lycopene Delivery to Prostate Cancer Cells

In Vitro Imaging of Lycopene Delivery to Prostate Cancer Cells

A Method for Fluorescent Diagnosis of Malignant Cutaneous Neoplasms Using Ytterbium Phthalocyanines

Cutaneous Oncology: Strategies for Melanoma Prevention, Diagnosis, and Therapy

Contact Info

Product

Resources

About