Ramophialophora chlamydospora, a new species from an alkaline lake of Wadi-El-Natron, Egypt

Moubasher, AH

doi:10.5943/ajom/2/1/5

Cited by 27 publications

(19 citation statements)

References 2 publications

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“…For DNA isolation, a small portion from fungal growth of 7-day-old colonies of Cladosporium isolates AUMC 10865, AUMC 11340 and AUMC 11366 grown on PDA at 25 °C were collected and transferred individually to 2 ml-Eppendorf tube. The DNA extraction was performed following CTAB method described in Moubasher, et al 57 .…”

Section: Dna Extractionmentioning

confidence: 99%

Production and Partial Purification of Pectinases Produced by Three Novel Cladosporium Species Isolated from Egypt

Moharram

Zohri

Hesham

et al. 2022

Preprint

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Based on phenotypic characters and sequencing of the internal transcribed spacers (ITS), 28S Large Subunit (LSU) and partial actin (ACT), three novel species of Cladosporium were described in this study as C. parasphaerospermum, C. chlamydosporum, and C. compactisporum. Activities of pectinases produced by the three strains were 5700, 3720, and 3480 U mg− 1 enzymes, at the optimum pH (6.0, 7.0, and 8.0) and temperature (35, 30, and 35 ºC), respectively. The ions potassium and manganese had a strong activating impact on the activity of C. compactisporum pectinase (124.4 and 172%, respectively). When tested under optimal conditions, EDTA and the other metal ions inhibited the activity of the pectinases in varied degrees. The strongest inhibitory effects of C. parasphaerospermum, C. chlamydosporum, and C. compactisporum pectinases were with Ni+ 2, Zn+ 2, and EDTA, respectively. Cladosporium species have a long history of biotechnological uses, and here we add another: pectinase makers. We offer three unique suppliers of low-temperature active fungal pectinases for biotechnological applications such as the management of Pectinacious wastes in the juice industry.

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Section: Dna Extractionmentioning

confidence: 99%

Production and Partial Purification of Pectinases Produced by Three Novel Cladosporium Species Isolated from Egypt

Moharram

Zohri

Hesham

et al. 2022

Preprint

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“…For DNA extraction, small part of the fungal mycelia of 7-day-old culture grown on Potato dextrose agar (PDA) was gathered and placed individually to Eppendorf tube to be applied according to (Moubasher et al, 2019).…”

Section: Molecular Identification Of Phosphate Solubilizing Strains A...mentioning

confidence: 99%

Improvement of Germination, Phosphate Efficiency, Antioxidants, Metabolic Products, and Yield of Wheat Plants by Aspergillus niger and Penicillium chrysogenum

et al. 2022

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I NCREASING the efficiency of phosphate fertilizer by biofertilizers is an interesting goal as a way to potentiate the phosphate-fertilizers.The present study includes the use of culture filtrate (CF) or conidial suspension (CS) of two isolated rhizospheric fungi (RF) in germination solution of wheat plants or pots filled with soils fertilized with superphosphate or rock phosphate. The RF were morphologically and genetically identified as Penicillium chrysogenum (AUMC 14100) and Aspergillus niger (AUMC 14260) that were able to produce indole acetic acid (IAA) and solubilize phosphate. Applying CF or CS of the strains separately or in consortium enhanced the germination percentage and vigor index of wheat plants. For pot experiment, CF or CS initiate their positive effect on plant in two ways: a) enhancement of soil properties (increment of organic matter, reduction of pH, enrichment the soil with higher soluble phosphate, calcium, and magnesium); b) stimulation of growth, biochemical status, nutrients content, and the yield of wheat plants. The applied biofertilizers enhanced chlorophyll, primary metabolites, phenolics, ascorbic acid, and tocopherol contents. The application of CF and/or CS exerted no oxidative damage on wheat rather H 2 O 2 or lipid peroxidation was reduced due to the activation of various antioxidant enzymes. Furthermore, the root and shoot tissues of treated wheat enriched with high contents of calcium, magnesium, and phosphate improve phosphate-related traits. All up-regulations under biofertilizers application reflected on high yield of wheat plants.The study recommends application of CF side by side to P-fertilizers for up-scaling their efficiency and enhancing plant development.

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“…For DNA isolation, a small portion from fungal growth of 7-day-old colonies of Cladosporium isolates AUMC 10865, AUMC 11340 and AUMC 11366 grown on potato dextrose agar (PDA) at 25°C were collected and transferred individually to 2 ml-Eppendorf tube. The DNA extraction was performed following CTAB method described in Moubasher et al 24 The universal primers ITS1 and ITS4 25 were used for amplification of the internal transcribed spacer (ITS) region. 26,9…”

Section: Isolatesmentioning

confidence: 99%

Production of Cocktail Enzymes by Three Cladosporium Isolates and Bioconversion of Orange Peel Wastes into Valuable Enzymes

Moharram¹,

Zohri²,

Hesham³

et al. 2021

J. Pure Appl. Microbiol.

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The current research demonstrates the biotechnological economization of accumulated and inefficiently used agro-industrial orange peel wastes to generate amylase, endoglucanase, exoglucanase, pectinase, and xylanase, industrially essential enzymes with growing demands in enzyme markets, from three Cladosporium isolates. In submerged fermentation (SmF) at 10°C, the isolate AUMC 10865 produced the highest level of amylase (4164 IU/gram dry substrate). Endoglucanase, exoglucanase and xylanase had development peaks (923 IU/gds, 2280 IU/gds, and 1646 IU/gds, respectively in case of Cladosporium sp. AUMC 11366. Pectinase produced the most (7840 IU/gds) in the strain AUMC 11340. At 30°C, the strain AUMC 11340 secretes the most amylase (4120 IU/gds), endoglucanase (2700 IU/gds) and xylanase (3220 IU/gds). Exoglucanase development reached the peak (8750 IU/gds) in the isolate AUMC 10865. The overall production (5570 IU/gds) was instead enhanced by pectinase in the AUMC 11366 isolate. In solid-state fermentation (SSF) at 10°C, the isolate AUMC 10865 outperformed the other two isolates producing 640.0 IU/gds amylase, 763.3 IU/gds endoglucanase, 771.0 IU/gds exoglucanase, 1273.23 IU/gds pectinase and 1062.0 IU/gds xylanase, while the isolate AUMC 11366 produced the least amount of 399.7 IU/gds, 410.0 IU/gds, 413.3 IU/gds, 558.7 IU/gds, and 548.0 IU/gds, respectively. At 30°C, the isolate AUMC 11340 was superiorly producing higher levels of amylase (973.3 IU/gds), endoglucanase (746.0 IU/gds), exoglucanase (1052.0 IU/gds), pectinase (1685.3 IU/gds) and xylanase (1340.0 IU/gds), whereas isolate AUMC 10865 generated the least amounts of amylase (556.7 IU/gds) and exoglucanase (452.7 IU/gfs), and the isolate AUMC 11366 produced the least endoglucanase (256.3 IU/gds), pectinase (857.7 IU/gfs) and xylanase (436.3 IU/gds) amounts.

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Ramophialophora chlamydospora, a new species from an alkaline lake of Wadi-El-Natron, Egypt

Cited by 27 publications

References 2 publications

Production and Partial Purification of Pectinases Produced by Three Novel Cladosporium Species Isolated from Egypt

Production and Partial Purification of Pectinases Produced by Three Novel Cladosporium Species Isolated from Egypt

Improvement of Germination, Phosphate Efficiency, Antioxidants, Metabolic Products, and Yield of Wheat Plants by Aspergillus niger and Penicillium chrysogenum

Production of Cocktail Enzymes by Three Cladosporium Isolates and Bioconversion of Orange Peel Wastes into Valuable Enzymes

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