Ran1 functions to control the Cdc10/Sct1 complex through Puc1.

Depending on environmental conditions, Schizosaccharomyces pombe can remain in the stationary phase or enter into either premitotic or premeiotic DNA synthesis. This decision point is known as Start. In the mitotic cell cycle, regulation of G 1 /S-specific gene expression is dependent upon the MBF (Mlu1 binding factor) complex, known to contain p85 cdc10 and p72res1 . Here we demonstrate that p73 res2 controls cell cycle progression via its participation in the MBF complex, interacting directly with both p85 cdc10 and p72 res1 . In contrast, when cells enter into meiosis, the MBF complex is disrupted, and p73 res2 shifts its regulatory function towards the transactivation of genes required for meiotic progression. These observations suggest that p73 res2 plays a pivotal role at Start and constitutes an example of a transcription factor involved in the control of both mitotic and meiotic progression.

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“…with data recently published by Caligiuri et al (8). It was suggested that the interaction between p85 cdc10 and p72 res1 was dependent on pat1.…”

Section: Cdc10supporting

confidence: 72%

The Fission Yeast Protein p73^res2 Is an Essential Component of the Mitotic MBF Complex and a Master Regulator of Meiosis

Ayté

Leis

DeCaprio

1997

Molecular and Cellular Biology

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“…3A; lanes 2-6), similar to that observed previously by others (Caligiuri et al 1997;Ayte et al 1997). The DSC1 detected in non G1 arrested meiotic cells is likely to represent the mitotic form of the complex, which under normal conditions would be expected to disappear, as fission yeast cells can only enter meiosis after nitrogen starvation (Yamamoto 1996).…”

Section: Identification Of a Dsc1-like Mcb Binding Complex In Meioticsupporting

confidence: 84%

DSC1-MCB regulation of meiotic transcription in Schizosaccharomyces pombe

2003

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Meiosis is initiated from the G1 phase of the mitotic cell cycle, and consists of pre-meiotic S-phase followed by two successive nuclear divisions. Here we show that control of gene expression during pre-meiotic S-phase in the fission yeast Schizosaccharomyces pombe is mediated by a DNA synthesis control-like transcription factor complex (DSC1), which acts upon M lu1 cell cycle box (MCB) promoter motifs. Several genes, including rec8+, rec11+, cdc18+, and cdc22+, which contain MCB motifs in their promoter regions, are found to be co-ordinately regulated during pre-meiotic S-phase. Both synthetic and native MCB motifs are shown to confer meiotic-specific transcription on a heterologous reporter gene. A DSC1-like transcription factor complex that binds to MCB motifs was also identified in meiotic cells. The effect of mutating and over-expressing individual components of DSC1 (cdc10+, res1+, res2+, rep1+ and rep2+) on the transcription of cdc22+, rec8+ and rec11+ during meiosis was examined. We found that cdc10+, res2+, rep1+ and rep2+ are required for correct meiotic transcription, while res1+ is not required for this process. This work demonstrates a role for MCB motifs and a DSC1-like transcription factor complex in controlling transcription during meiosis in fission yeast, and suggests a mechanism for how this specific expression occurs.

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“…More prominent mRNA regulation was found in the fibroblastic cell lines CV1 and NIH3T3, with peak mRNA levels at the end of S-phase. In all cases the expression pattern coincided with that of cyclin B and Cdc2/cyclin B activation [75].…”

Section: Thymosin Family Members and Cell Cyclementioning

confidence: 54%

Gene expression profile of HIV-1 Tat expressing cells: a close interplay between proliferative and differentiation signals

et al. 2002

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Background: Expression profiling holds great promise for rapid host genome functional analysis. It is plausible that host expression profiling in an infection could serve as a universal phenotype in virally infected cells. Here, we describe the effect of one of the most critical viral activators, Tat, in HIV-1 infected and Tat expressing cells. We utilized microarray analysis from uninfected, latently HIV-1 infected cells, as well as cells that express Tat, to decipher some of the cellular changes associated with this viral activator.

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Ran1 functions to control the Cdc10/Sct1 complex through Puc1.

Cited by 9 publications

References 67 publications

The Fission Yeast Protein p73^res2 Is an Essential Component of the Mitotic MBF Complex and a Master Regulator of Meiosis

The Fission Yeast Protein p73^res2 Is an Essential Component of the Mitotic MBF Complex and a Master Regulator of Meiosis

DSC1-MCB regulation of meiotic transcription in Schizosaccharomyces pombe

Gene expression profile of HIV-1 Tat expressing cells: a close interplay between proliferative and differentiation signals

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Ran1 functions to control the Cdc10/Sct1 complex through Puc1.

Cited by 9 publications

References 67 publications

The Fission Yeast Protein p73res2 Is an Essential Component of the Mitotic MBF Complex and a Master Regulator of Meiosis

The Fission Yeast Protein p73res2 Is an Essential Component of the Mitotic MBF Complex and a Master Regulator of Meiosis

DSC1-MCB regulation of meiotic transcription in Schizosaccharomyces pombe

Gene expression profile of HIV-1 Tat expressing cells: a close interplay between proliferative and differentiation signals

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Product

Resources

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The Fission Yeast Protein p73^res2 Is an Essential Component of the Mitotic MBF Complex and a Master Regulator of Meiosis

The Fission Yeast Protein p73^res2 Is an Essential Component of the Mitotic MBF Complex and a Master Regulator of Meiosis