2016
DOI: 10.1038/nmeth.4033
|View full text |Cite
|
Sign up to set email alerts
|

Random-access scanning microscopy for 3D imaging in awake behaving animals

Abstract: Understanding how neural circuits process information requires rapid measurements of activity from identified neurons distributed in 3D space. Here we describe an acousto-optic lens two-photon microscope that performs high-speed focusing and line scanning within a volume spanning hundreds of micrometers. We demonstrate its random-access functionality by selectively imaging cerebellar interneurons sparsely distributed in 3D space and by simultaneously recording from the soma, proximal and distal dendrites of ne… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

1
126
0
2

Year Published

2016
2016
2024
2024

Publication Types

Select...
5
3
2

Relationship

0
10

Authors

Journals

citations
Cited by 132 publications
(129 citation statements)
references
References 22 publications
1
126
0
2
Order By: Relevance
“…1b,c) by comparing, at equal laser power, the SNR of calcium signals generated by single APs and trains of few APs in the same individual GCaMP6-expressing neurons under the two experimental configurations (scanning and scanless). Random-access two-photon imaging is currently considered the gold standard for high-speed two-photon imaging in the mouse brain in vivo 910485152. Although random-access systems could theoretically scan ~167009 or ~5430010 different positions in one second, thus reaching the performances achieved in the present study (i.e., 47 cells at 1 kHz), previous studies have not achieved these performances at extremely high acquisition rates (e.g., 1 kHz)9104851.…”
Section: Discussionmentioning
confidence: 53%
“…1b,c) by comparing, at equal laser power, the SNR of calcium signals generated by single APs and trains of few APs in the same individual GCaMP6-expressing neurons under the two experimental configurations (scanning and scanless). Random-access two-photon imaging is currently considered the gold standard for high-speed two-photon imaging in the mouse brain in vivo 910485152. Although random-access systems could theoretically scan ~167009 or ~5430010 different positions in one second, thus reaching the performances achieved in the present study (i.e., 47 cells at 1 kHz), previous studies have not achieved these performances at extremely high acquisition rates (e.g., 1 kHz)9104851.…”
Section: Discussionmentioning
confidence: 53%
“…"The limitation to speed is the speed of sound across crystal, basically, " Silver says. The technique still isn't ideal for quickly imaging every neuron in a volume, he says, but it can move from one region to the next in about 25 microseconds 7 . That makes it useful for viewing all of a sparse population, such as inhibitory interneurons in a volume of brain, he suggests.…”
Section: Advancing Microscopymentioning
confidence: 99%
“…The major advancement in measuring Ca 2+ signals was the invention and the application of two-photon microscopy in the nervous system (Denk et al, 1990; Yuste and Denk, 1995). Over many years, particularly with the help of the continuous development of Ca 2+ indicators, two-photon Ca 2+ imaging has become widely used for detecting neural activities on multiple scales ranging from networks to single synapses in both anesthetized and behaving animals (Stosiek et al, 2003; Chen et al, 2011, 2013; Nadella et al, 2016; Szalay et al, 2016). Another commonly used approach for in vivo brain Ca 2+ imaging is based on the use of charged coupled detector/complementary metal-oxide-semiconductor-based cameras, which are particularly useful for recording large-field Ca 2+ dynamics in the superficial cortical layers (Berger et al, 2007).…”
Section: Introductionmentioning
confidence: 99%