Random amplified microsatellites (RAMS) — a novel method for characterizing genetic variation within fungi

Hantula, Jarkko; Dusabenyagasani, M.; Hamelin, Richard

doi:10.1111/j.1439-0329.1996.tb00720.x

Cited by 193 publications

(136 citation statements)

References 11 publications

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“…Primers were selected based on reference data (Hantula et al, 1996;McGregor et al, 2000). Sixty primers were tested.…”

Section: Isolation Of Genomic Dna and Primer Selectionmentioning

confidence: 99%

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Sulima¹,

Przyborowski²

2013

Acta Biologica Cracoviensia Series Botanica

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This study used ISSR markers to assess the genetic diversity of a collection of 15 genotypes of Salix purpurea and 6 interspecific hybrids, employing 40 of 60 tested ISSR primers generating polymorphic amplification products. The PCR-ISSR method was adapted for S. purpurea by optimizing the annealing temperature for each primer. The polymorphism index of ISSR amplification products was 91.8% for all studied genotypes and 70.4% for S. purpurea genotypes. Nei's genetic identity statistics ranged from 0.538 to 0.958. Nei's genetic distance values were used to build a dendrogram (UPGMA) for the investigated genotypes. The dendrogram shows five clusters, and principal coordinate analysis yielded nearly the same genetic relationships among the studied genotypes. The results confirm the usefulness of ISSR markers for determining genetic diversity in S. purpurea.

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“…Primers were selected based on reference data (Hantula et al, 1996;McGregor et al, 2000). Sixty primers were tested.…”

Section: Isolation Of Genomic Dna and Primer Selectionmentioning

confidence: 99%

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Sulima¹,

Przyborowski²

2013

Acta Biologica Cracoviensia Series Botanica

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“…Genetic polymorphism of isolates was determined by RAMS method (Random Amplified Microsatellites) (Hantula et al 1996;Zietkiewicz et al 1994). Eight primers were tested, among which most relevant ones were selected on the basis of electrophoretic profiles and repeatability of results (Table 3).…”

Section: Molecular Analysesmentioning

confidence: 99%

Genetic variability of Chalara fraxinea, dieback cause of European ash (Fraxinus excelsior L.)

2010

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A total of 159 colonies of Chalara fraxinea were isolated between 2005 and 2006 from dying trees of European ash (Fraxinus excelsior L.) aged between 3 and 10 years. They derived from five regions of Poland differing by geographic location and climatic conditions. On the basis of 90 RAMS markers, pathogen intra-and inter-population variability, as well as its dependency on geographic distance and climatic conditions in the regions of strain origin, was analysed. The applied measures of intrapopulation genetic variability (genetic distance, Nei's unbiased diversity, Shannon's Information Index and percentage of polymorphic loci) allowed for differentiation of two strain groups: the first deriving from lowlands and the second from uplands and mountainous areas. Strains in lowlands were characterised by smaller number of markers, smaller number of polymorphic loci and smaller intrapopulation genetic variability. Positive and statistically significant correlation was shown between variability of isolates and elevation of regions above sea level. Pair-wise genetic distances between groups of isolates (Nei's unbiased genetic distance) from particular regions were not significantly correlated with the corresponding geographic distances. On the basis of AMOVA, it was shown that 85% of variability was within-region differences and 2% betweenregion differences, whereas differences between lowlands and uplands were 13%. Principal Components Analysis (PCA) for the investigated regions confirmed the results from Nei's genetic distance matrix.

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“…W wyniku amplifikacji wielu miejsc w genomie P. gigantea powsta³ bowiem szereg produktów PCR (tab. 5), charakterystyczny dla ka¿dego szczepu z danego preparatu (Hantula et al 1996;Vainio et al 1998). Stwierdzono jednak¿e, ¿e rozró¿nienie preparatów Rotstop miêdzy sob¹ na podstawie markerów RAMS jest utrudnione i polega na ocenie intensywnooeci oewiecenia markera o masie ok. 400 pz, w odniesieniu do wzorca (Pilot 2005;Rutkowski 2005).…”

Section: Omówienie Wyników I Wnioskiunclassified

Evaluating the persistence of Phlebiopsis gigantea (Fr.: Fr.) Jülich mycelium and fruiting bodies in pine stumps after root-rot protection treatments

Małecka¹,

Żółciak²,

Sikora³

et al. 2012

Forest Research Papers

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Ocena wystêpowania grzybni i owocnikówAbstract. Commercial preparations Rotstop and PgSuspension, used in Scandinavia and the United Kingdom, are EUlicensed biocontrol agents against root rot that contain isolates of the fungus Phlebiopsis gigantea. The composition of these two products differs from that of PgIBL, previously use in Poland, hence the need to verify their effectiveness under Polish site and stand conditions. Stumps were treated with the commercial products and subsequently checked after one year for the persistence of mycelia and fruiting bodies. Molecular methods were used on the mycelia present in the stumps to confirm that their genetic identity was consistent with fungal isolates from the preparations. Treatments with EU-licensed preparations gave satisfactory results -in about 70-80% of the stumps mycelia were present under the bark. Inoculation with Rotstop preparations was most effective during autumn 2008 (effectiveness of 82%), while PgSuspension application was most effective during spring 2008 (effectiveness of 77%). The development of the mycelia in pine stumps from the two preparations investigated (containing preserved dormant spores) was similar to that of preparation PgIBL (formulated from living mycelium within a growing medium), in terms of their relative effectiveness measured as the percentage of stumps supporting under-bark mycelia (which ranged between 70-90%, depending on the treatment term).

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Random amplified microsatellites (RAMS) — a novel method for characterizing genetic variation within fungi

Cited by 193 publications

References 11 publications

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Genetic variability of Chalara fraxinea, dieback cause of European ash (Fraxinus excelsior L.)

Evaluating the persistence of Phlebiopsis gigantea (Fr.: Fr.) Jülich mycelium and fruiting bodies in pine stumps after root-rot protection treatments

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