Random amplified polymorphic DNA analysis of Anopheles nuneztovari (Diptera: Culicidae) from Western and Northeastern Colombia

Posso, Carmen Elisa; Obando, Ranulfo González; Cárdenas, Héiber; Gallego, Gerardo; Duque, Myriam C.; Suárez, Marco Fidel

doi:10.1590/s0074-02762003000400007

Cited by 23 publications

(5 citation statements)

References 17 publications

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“…The expected heterozygosity of Argyresthia conjugella in our study, was slightly higher than in SSR analyses of Metrioptera roeselii ‘0.61’ [ 21 ], Leptomyrmex pallens ‘0.51’ [ 43 ] and Cerambyx cerdo ‘0.63’ [ 44 ]. However, the expected heterozygosity was much higher than was previously detected by SSRs in Plutella xylostella ‘0.35’ [ 11 ], Anopheles nuneztovari (Culicidae) ‘0.34’ [ 45 ], Glossina pallidipes ‘0.35’ [ 46 ], Bombus distinguendus ‘0.38’ [ 47 ] and Epirrita autumnata ‘0.43’ [ 35 ]. We may speculate if the high genetic variation may be caused by previous population fragmentation and/or multiple founders as a result of the life cycle, outbreak cycles, lower migration distance as well as the topography in Scandinavia and/or the use of insecticides on selected parts of the population.…”

Section: Discussionmentioning

confidence: 96%

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

et al. 2018

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The apple fruit moth Argyresthia conjugella (Lepidoptera, Yponomeutidae) is a seed predator of rowan (Sorbus aucuparia) and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2–4 years, and apple (Malus domestica) functions as an alternative host, resulting in economic loss in apple crops in inter-mast years. We have used Illumina MiSeq sequencing to identify a set of 19 novel tetra-nucleotide short tandem repeats (STRs) in Argyresthia conjugella. Such motifs are recommended for genetic monitoring, which may help to determine the eco-evolutionary processes acting on this pest insect. The 19 STRs were optimized and amplified into five multiplex PCR reactions. We tested individuals collected from Norway and Sweden (n = 64), and detected very high genetic variation (average 13.6 alleles, He = 0.75) compared to most other Lepidoptera species studied so far. Spatial genetic differentiation was low and gene flow was high in the test populations, although two non-spatial clusters could be detected. We conclude that this set of genetic markers may be a useful resource for population genetic monitoring of this economical important insect species.

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Section: Discussionmentioning

confidence: 96%

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

et al. 2018

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“…The results obtained in this study, using primer A10, denoted the existence of gene flow within the study population (old and new transmission areas of ACL). Specimens collected in different ecotypes and different municipalities were grouped in the same phenetic subgroup with varying degrees of similarity, as the results obtained by similar studies involving Anopheles Meigen, 1818 species (Posso et al, 2003;González et al, 2007). The evidence of gene flow is denoted not only by analysis of the dendrogram, but also considering the observation that sand flies from different municipalities showed the same genetic profile.…”

Section: Discussionmentioning

confidence: 91%

GENETIC DIVERSITY OF Lutzomyia (Nyssomyia) intermedia IN AN ENDEMIC AREA OF AMERICAN CUTANEOUS LEISHMANIASIS, STATE OF PARANÁ, BRAZIL

Gonçalves¹,

Castro

Luz

et al. 2021

Acta biol. Colomb.

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Lutzomyia intermedia (Diptera: Psychodidae) features as one of the main vectors that are involved in the transmission of American cutaneous leishmaniasis (ACL) in the Neotropical region. However, genetic studies involving this taxon are still incipient and important for understanding the level of variability of different populations, their role, and implications as vectors. The aim of this study was to determine the level of genetic diversity of L. intermedia present in the Ribeira River Valley, an area of ACL transmission in the state of Paraná, Brazil, through the Random amplified polymorphic DNA (RAPD). Two municipalities were chosen to collect sand flies: Cerro Azul (new transmission area of the ACL) and Adrianópolis (endemic area of the ACL). The insects were captured in the house, in the peridomicile and in the wild (forest). Two of the used markers made it possible to estimate the polymorphism of the studied populations, resulting in 40 genotypes, most of them from peridomicile. The dendrogram generated by the analysis with the primer A10 showed different degrees of similarity, suggesting that there may be gene flow in the studied populations. The Principal Coordinate Analysis (PCO) with the A2 primer, was useful in grouping L. intermedia according to its ecological and geographical origin. There was no distinction between the lineages composing the L. intermedia complex. The results of this study, with the record of great genotypic diversity in L. intermedia, may contribute to explain the maintenance of the life cycle of Leishmania braziliensis (Kinetoplastida: Trypanosomatidae) in the region.

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“…When examining the RAPD studies in the literature on Anopheles species, differences are seen in terms of various values. Posso et al (2003) found a genetic diversity value of 0.34 in the results they obtained from the RAPD markers used to analyze the genetic diversity and structure of 119 samples from three An. nuneztovari populations they sampled from Colombia.…”

Section: Discussionmentioning

confidence: 97%

“…Genetic markers based on nucleic acids can be used to study species characterization, genetic diversity, phylogenetic relationships, and insect identification (Rita et al, 2014). Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and other nonspecific amplification methods are quite useful for analyzing genetic variation within species due to the help they provide in quickly obtaining vast genetic information (Posso et al, 2003). RAPD-PCR is a method that allows one to amplify the template genomic DNA using random primers.…”

Section: Introductionmentioning

confidence: 99%

“…Vagus, and An. culucifacies (Wilkerson et al, 1993;Favia et al, 1994;Dimopoulos et al, 1996;Kengne et al, 2001;Elisa Posso et al, 2003Pinedo-Cancino et al, 2006;Silva-Do-Nascimento et al, 2006;Gonzalez et al, 2007;Rita et al, 2014;Tyagi et al, 2015). However, no study is found in This study investigates the intra-and inter-population genetic diversity of natural An.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Structure of Anopheles sacharovi (Diptera: Culicidae) Populations from Türkiye

GÜNENÇ¹,

MANAP²,

ÇELİKKOL³

et al. 2023

tjbc

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Objective: The Anopheles maculupennis complex is known to be a vector of malaria in Türkiye. Anopheles sacharovi is a member of the Anopheles maculipennis complex and is a major vector of malaria in Europe and the Middle East capable of transmitting both Plasmodium vivax and P. falciparum. The genetic diversity and population structure of the six populations of Anopheles sacharovi from Türkiye are studied here using the random amplified polymorphic DNA (RAPD) marker. Materials and Methods: Anopheles sacharovi samples were collected from Muğla, Aydın, İzmir, Çanakkale, Balıkesir, and Denizli. Total DNA was extracted using the Lifton method, and a total of 120 individuals were screened using six RAPD primers. Results: A total of 300 loci were observed in the six primers. Very close genetic diversity was observed in the studied populations. The number of alleles (n a ) observed for all populations was 1.55 ± 0.49, and the mean number of effective alleles (n e ) was 1.23 ± 0.31. The ratio of polymorphic loci for all populations ranged between 50.33%-60.33%, and Nei's genetic diversity (h) ranged between 0.1253-0.1576. The gene flow level (N m ) was 2.08, and the genetic differentiation value (G st ) was 0.19. The conclusions from the unweighted pair group arithmetic mean analysis (UPGMA) was inclined to be homogeneous on the whole, with the İzmir population being clearly separated from the rest. Conclusion: Regional environmental conditions such as human-mediated transport, agricultural implementations, and discrepancies in vector control strategies might be considered agents in forming the genetic structure of this species in İzmir. Understanding the gene flow rates and phylogenetic relationships between vector species are very important for applying sustainable and effective pest management. This study ensures helpful knowledge for better understanding the population genetic structure of An. sacharovi populations in Türkiye.

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Random amplified polymorphic DNA analysis of Anopheles nuneztovari (Diptera: Culicidae) from Western and Northeastern Colombia

Cited by 23 publications

References 17 publications

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers

GENETIC DIVERSITY OF Lutzomyia (Nyssomyia) intermedia IN AN ENDEMIC AREA OF AMERICAN CUTANEOUS LEISHMANIASIS, STATE OF PARANÁ, BRAZIL

Genetic Structure of Anopheles sacharovi (Diptera: Culicidae) Populations from Türkiye

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