Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans cultivated in brazilian cerrado soils

Oliveira, Isnia Aparecida de; Vasconcellos, Maria José; Seldin, Lucy; Paiva, Edílson; Vargas, M. A. T.; Sá, Nadja Maria Horta de

doi:10.1590/s1517-83822000000100010

Cited by 10 publications

(5 citation statements)

References 12 publications

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“…Some strains isolated from the same site were classified in different clusters. According to de Oliveira et al [3] isolates of R. tropici and R. leguminosarum bv. phaseoli clustered together in relation to the soil characteristics of each sample site.…”

Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

Stefan

Roșu

Stedel

et al. 2015

Acta Biologica Hungarica

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The application of commercial rhizobial inoculants to legume crops is proving to be an alternative to synthetic fertilizer use. The challenge for sustainable agriculture resides in the compatibility between crop, inoculants and environmental conditions. The evaluation of symbiotic efficiency and genetic diversity of indigenous rhizobial strains could lead to the development of better inoculants and increased crop production. The genetic variability of 32 wild indigenous rhizobial isolates was assessed by RAPD (Random Amplified Polymorphic DNA). The strains were isolated from red clover (Trifolium pratense L.) nodules from two distinct geographical regions of Northern and Eastern Romania. Three decamer primers were used to resolve the phylogenetic relationships between the investigated isolates. Cluster analysis revealed a high diversity; most strains clustered together based on their geographical location.

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Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

Stefan

Roșu

Stedel

et al. 2015

Acta Biologica Hungarica

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“…Different techniques are now available to subtype bacteria up to strain level, such as restriction fragment length polymorphism (RFLP), plasmid profiling, ribotyping, amplified ribosomal DNA restriction analysis (ARDRA), pulsed field gel electrophoresis (PFGE), and randomly amplified polymorphic DNA (RAPD). Different PGPR have already been characterized by one or more of these methods (Oliveira et al 2000;von der Weid et al 2000;Depret and Laguerre 2008;Monteiro et al 2009; and many others). For a detailed description of these methods, the reviews by Vandamme et al (1996), Prakash et al (2007), Rodríguez-Díaz et al (2008), and Logan et al (2009) can be referred.…”

Section: Genetic Approachesmentioning

confidence: 99%

Plant Growth Promoting Rhizobacteria: Fundamentals and Applications

Figueiredo

Seldin

Araújo

et al. 2010

Microbiology Monographs

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168

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“…[11] proposed this method for identification and phylogenetic grouping of Rhizobium isolates. RAPD PCR technique provides reliable information on the diversity of Rhizobium populations in soils [12].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Diversity of Rhizobia Nodulating Faba Bean from Various Egyptian Locations

Hewedy¹

2014

J Bioprocess Biotech

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Thirteen Rhizobial isolates from root nodules of Faba beans (Vicia faba L.) were obtained from eleven governorates, representing different agro-ecological, agro-climatic and soil conditions in Egypt. A polyphasic approach, including phenotypic and genotypic techniques were used to study their diversity. Rhizobium isolates were examined for their ability to tolerate salt stress and antibiotic resistance. We used four NaCl concentrations ranging from 0.1%, 1%, 3% and 5% in liquid media and plates, moreover we used 24 different antibiotic disks to determine resistance or sensitive among the tested isolates on plates. The obtained results after salt tolerance and antibiotic response of the R. leguminosarium strains showed that the isolate No. RL9 was the superior strain for salt tolerance. The following strains for salt tolerance were the isolates (RL4) and (RL12), although the isolate No. RL11 was the superior strain for antibiotic resistant then isolates No. RL 13 and Rh3. Growth of all isolates were inhibited above 3% NaCl except five isolates RL4, RL9, RL10, RL12 and RL 13. Also the antibiotic resistance of the isolated strains showed a high level of resistance against Cefoxitin (FOX 30) and Cefuroxime (Cxm 30). The genetic diversity was studied using RAPD-PCR technique and we used specific primer (nodC) amplification. In this study we found that OPE 15 and OPG 04 primers showed the highest polymorphism level among the tested isolates, however OPJ 20 and OPC 06 primers showed the lowest polymorphism level.

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Random amplified polymorphic DNA analysis of effective Rhizobium sp. associated with beans cultivated in brazilian cerrado soils

Cited by 10 publications

References 12 publications

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

RAPD-inferred genetic variability of some indigenousRhizobium leguminosarumisolates from red clover (Trifolium pratenseL.) nodules

Plant Growth Promoting Rhizobacteria: Fundamentals and Applications

Phenotypic and Genotypic Diversity of Rhizobia Nodulating Faba Bean from Various Egyptian Locations

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