Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey

Ozbey, Gokben; Ertaş, Hasan; Muz, Adile

doi:10.17221/5660-vetmed

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…Again, strains RefA, RefB and RefE clustered together in the neighbor-joining tree, and strains RefD and RefH showed close relationship to each other. Likewise, Ozbey et al [ 19 ] found that some strains of serotype A shared their RAPD profile with those of serotype B and E. Only the serotype reference strain C (this study RefC) had a unique RAPD profile. The relationship between strains of serotypes A and B as well as the distinct RAPD profile of serotype C were confirmed by Waldow [ 20 ].…”

Section: Resultssupporting

confidence: 59%

“…Serotype-specific relationships have been indicated by previous studies using PFGE [ 21 ], 16S rRNA and Or01 [ 15 ] gene analyses or RAPD-PCR [ 19 , 20 ] for characterization of ORT. In the PFGE analysis serotype strains A-Q were identical with RefA-RefQ used in the present study.…”

Section: Resultsmentioning

confidence: 99%

“…Due to the lack of serotype information, a possible relationship was not investigated [ 18 ]. A subsequent RAPD analysis of 6 field strains from chickens in Turkey [ 19 ] included strains of serotypes A to E (this study RefA-RefE), but did not find clear genotype-serotype correlations. Similar results were obtained for 117 field strains of different serotypes from France and Germany by comparing their RAPD profiles with those of different serotype reference strains (this study RefA-RefQ) [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of the Recently Emerged Poultry Pathogen Ornithobacterium rhinotracheale by Multilocus Sequence Typing

et al. 2016

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Ornithobacterium rhinotracheale (ORT) is an economically important bacterial pathogen of turkeys and chickens worldwide. Since its first detection, a variety of typing methods have been used to gain basic knowledge about the bacterial population structure, an issue that still needs to be addressed. Serological characterization revealed at least 18 different serotypes (A-R) with ORT of serotype A to be predominate among poultry. This study aimed to establish a multilocus sequence typing (MLST) scheme for ORT that could easily be used by other laboratories and allows for worldwide comparison of sequence data. For this purpose, 87 ORT strains from different poultry hosts, geographical origins, years of isolation and serotypes were included in the analysis to identify correlations. Fourteen different sequence types (ST) were found. The most common ST1 was identified in 40 ORT strains from turkeys and chickens on 4 continents and in 3 different European countries. Together with ST9, both STs represented over three quarters (77%) of ORT strains used in the MLST analysis and included strains of frequently cross-reacting ORT serotypes A, E and I. Nine STs were only represented by one ORT strain and might indicate possible avian host, disease or serotype-specific relationships. In contrast, discrepancies between serotype and phylogenetic relatedness were clearly demonstrated by ORT strains that belonged to identical serotypes but differed in their ST. The overall identified low genetic diversity among strains isolated from turkeys and chickens independent of host and geographical origins suggests that ORT has only recently been introduced into domestic poultry and dispersed worldwide.

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Section: Resultssupporting

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of the Recently Emerged Poultry Pathogen Ornithobacterium rhinotracheale by Multilocus Sequence Typing

et al. 2016

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“…However, no correlation with the origin of the isolates was found. Ozbey et al (2005) reported high genetic diversity among O. rhinotracheale isolates from chickens in Turkey with an RAPD assay using the OPG11 primer. However, they used only six field isolates, which represented six different profiles, so the number of isolates was too low to draw such farreaching conclusions.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Ornithobacterium rhinotracheale field isolates from Hungary

et al. 2017

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Ornithobacterium rhinotracheale is a widely distributed rod-shaped Gram-negative bacterium that infects several avian species including chickens and turkeys. It is associated with respiratory signs, growth retardation, mortality, and reduced egg production, thus causing severe economic losses to the poultry industries. In this study, 37 field isolates of O. rhinotracheale, collected from various locations in Hungary between 1997 and 2015, were identified and characterized by the analysis of partial 16S rRNA gene sequences, enterobacterial repetitive intergenic consensus (ERIC)-PCR, and random amplified polymorphic DNA (RAPD) PCR assays with the OPG11, OPH19, and M13 primers. Most of the field isolates were serotype A, one was serotype B, and four were serotype D. One isolate could not be typed with antisera against serotypes A-E. In a phylogenetic analysis of the 16S rRNA sequences, the isolates formed two clusters. Thirteen distinct patterns were identified with ERIC-PCR, and the RAPD assay with the M13 primer assigned the isolates to 10 different patterns. The other two RAPD assays were unsuitable for distinguishing and grouping the isolates. Neither ERIC type nor RAPD pattern correlated with the place or year of isolation. However, the strains isolated from chickens were more heterogeneous on ERIC-PCR than the isolates recovered from turkeys. In this study, ERIC-PCR was the most discriminatory method for investigating the genetic diversity of O. rhinotracheale isolates.

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Serologic prevalence of Ornithobacterium rhinotracheale infections in broilers and broiler breeder chickens in Kermanshah Province, west of Iran

Rahimi

2012

Comp Clin Pathol

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Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey

Cited by 6 publications

References 16 publications

Molecular Characterization of the Recently Emerged Poultry Pathogen Ornithobacterium rhinotracheale by Multilocus Sequence Typing

Molecular Characterization of the Recently Emerged Poultry Pathogen Ornithobacterium rhinotracheale by Multilocus Sequence Typing

Characterization of Ornithobacterium rhinotracheale field isolates from Hungary

Serologic prevalence of Ornithobacterium rhinotracheale infections in broilers and broiler breeder chickens in Kermanshah Province, west of Iran

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