Random Amplified Polymorphic DNA (RAPD) and Derived Techniques

Babu, K. Nirmal; Sheeja, T. E.; Divakaran, Minoo; Rajesh, M. K.; Samsudeen, K.; Suraby, Erinjery Jose; Kumar, I. P. Vijesh

doi:10.1007/978-1-0716-0997-2_13

Cited by 22 publications

(18 citation statements)

References 92 publications

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“…Molecular methods for the characterization of microorganisms and of relationships between isolates may be classified into three main groups: PCR-based, restriction enzymes-based, and sequencing-based. PCR-based methods (AFLP, RAPD, VNTR) [ 64 , 65 , 66 ] ( Box 1 ) are commonly used for pathogen typing and as genetic markers for mapping analysis in aquaculture species. The development of PCR-based amplified fragment length polymorphism (AFLP) analysis is a key advancement in simple typing techniques.…”

Section: Pathogen Characterizationmentioning

confidence: 99%

What Can Genetics Do for the Control of Infectious Diseases in Aquaculture?

Sciuto

Colli

Fabris³

et al. 2022

Animals

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Infectious diseases place an economic burden on aquaculture and a limitation to its growth. An innovative approach to mitigate their impact on production is breeding for disease resistance: selection for domestication, family-based selection, marker-assisted selection, and more recently, genomic selection. Advances in genetics and genomics approaches to the control of infectious diseases are key to increasing aquaculture efficiency, profitability, and sustainability and to reducing its environmental footprint. Interaction and co-evolution between a host and pathogen can, however, turn breeding to boost infectious disease resistance into a potential driver of pathogenic change. Parallel molecular characterization of the pathogen and its virulence and antimicrobial resistance genes is therefore essential to understand pathogen evolution over time in response to host immunity, and to apply appropriate mitigation strategies.

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Section: Pathogen Characterizationmentioning

confidence: 99%

What Can Genetics Do for the Control of Infectious Diseases in Aquaculture?

Sciuto

Colli

Fabris³

et al. 2022

Animals

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“…The RAPD marker application protocol involves arbitrary oligonucleotide short primers usually 8 to 15 nucleotides in length that by PCR, randomly amplify DNA sections of large genomic DNA ( Babu et al., 2021 ). In RAPD analysis, prior DNA sequence information is not an important requirement because of the use of arbitrary primers.…”

Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

“…Another important shortcoming is that data quality is limited because RAPD is a dominant marker. Recent modifications have, however, improved the RAPD technique into more efficient marker methods like SCAR, SRAP and CAPS ( Yang et al., 2014 ; Babu et al., 2021 ). These improved marker variants of RAPDs overcome the associated disadvantages of RAPDs and complement the efficiency in the applications of the marker.…”

Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Amiteye

2021

Heliyon

131

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The concepts, methodologies and applications of some of the major molecular or DNA markers commonly used in plant science have been presented. The general principles of molecular marker techniques have been elucidated with detailed explanation of some notable basic concepts associated with marker applications: marker polymorphism, dominant or co-dominant mode of inheritance, agronomic trait-marker linkage, genetic mutations and variation. The molecular marker methods that have been extensively reviewed are RFLP, RAPD, SCAR, AFLP, SSR, CpSSR, ISSR, RAMP, SAMPL, SRAP, SSCP, CAPS, SNP, DArT, EST, and STS. In addition, the practicality of the retrotransposon-based marker methods, IRAP, REMAP, RBIP, and IPBS, have been discussed. Moreover, some salient characteristics of DNA markers have been compared and the various marker systems classified as PCR-or non-PCR-based, dominantly or co-dominantly inherited, locus specific or non-specific as well as at the levels of marker polymorphism and efficiency of marker reproducibility. Furthermore, the principles and methods of the following DNA markers have been highlighted: Penta-primer amplification refractory mutation system (PARMS), Conserved DNA-Derived Polymorphism (CDDP), P450-based analogue (PBA) markers, Tubulin-Based Polymorphism (TBP), Inter-SINE amplified polymorphism (ISAP), Sequence specific amplified polymorphism (S-SAP), Intron length polymorphisms (ILPs), Inter small RNA polymorphism (iSNAP), Direct amplification of length polymorphisms (DALP), Promoter anchored amplified polymorphism (PAAP), Target region amplification polymorphism (TRAP), Conserved region amplification polymorphism (CoRAP), Start Codon Targeted (SCoT) Polymorphism, and Directed Amplification of Minisatellite DNA (DAMD). Some molecular marker applications that have been recently employed to achieve various objectives in plant research have also been outlined. This review will serve as a useful reference resource for plant breeders and other scientists, as well as technicians and students who require basic know-how in the use of molecular or DNA marker technologies.

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“…Лаванда является одним из основных возделываемых на юге России эфиромасличных растений [135]. Широкое применение этого растения связано с наличием в его соцветиях эфирного масла, кумаринов, дубильных и других веществ [371,483,558]. Лаванда возделывается главным образом для получения эфирного масла, основными компонентами которого являются линалилацетат (30-50%) и линалоол (10-20%), а также содержатся гераниол, нерол, лимонен, фурфурол и другие компоненты.…”

Section: глава 2 лаванда узколистная (Lavandula Angustifolia Mill)unclassified

“…Эфирное масло используется в пищевой и парфюмерно-косметической промышленности, в керамическом, лакокрасочном, фарфоровом производствах [151,123]. В медицине его применяют как противомикробное, ранозаживляющее, антиоксидантное, успокаивающее и спазмолитическое средство, рекомендуемое при ревматических, желудочных и многих других заболеваниях [165,483]. Лаванда используется как медоносное, декоративное, а также противоэрозийное растение, возделываемое на склоновых землях.…”

Section: глава 2 лаванда узколистная (Lavandula Angustifolia Mill)unclassified

Bionechnology of Essential Oil Plants: Creation of New Forms and Micropropagation in Vitro

Yegorova¹

2021

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This monograph presents the results of many years research devoted to the development of complex biotechnological systems of creation the new forms and clonal micropropagation of such essential oil plants as lavender (Lavandula angustifolia Mill.), sage (Salvia sclarea L.), coriander (Coriandrum sativum L.), fennel (Foeniculum vulgare Mill.), anise (Anisum vulgare Gaerth.), yarrow (Achillea spp.), essential oil rose (Rosa spp.) and geranium (Pelargonium spp.). These biotechnologies are based on the investigation of features of isolated organs, callus tissues cultivation and morphogenesis induction in vitro. Chapter I deals with the general information on the methods of cell engineering that allow creating new plant genotypes and propagating them in vitro. The following Chapters summarize biotechnological research carried out at the Research Institute of Agriculture of Crimea (the former the Institute of Essential Oil and Medicinal Plants), using aromatic plant species, mainly grown in southern Russia (lavender, coriander, sage, fennel, rose yarrow etc.). The conditions of callus cultures obtaining from different explants were optimized and peculiarities of exogenous and endogenous factors effects on the callusogenesis efficiency of studied essential oil plants were detected. The methods of plant regeneration from calluses of different passages for more than fifty cultivars and samples of lavender, sage, coriander, fennel, anise, geranium, yarrow have been developed. The peculiarities of genotype, hormonal composition of culture medium, type of explant and number of passage influence on the morphogenesis induction have been determined. The means, 6 which permit to increase the frequency of organogenesis and somatic embryogenesis of callus cultures and to regenerate the plants during 1-3 years, were revealed. Furthermore, the method for creating essential oil rose hybrids using embryo culture and clonal micropropagation in vitro was developed. The peculiarities of abiotic stress factors effect at different biotechnological objects (calluses with and without morphogenesis and zygotic embryos) have been revealed. For the first time, the selective systems in vitro for screening forms with resistance to salinity, osmotic and low temperature stress have been elaborated for lavender, sage, geranium, coriander. For lavender, geranium, yarrow, fennel, rose and sage, methods of clonal micropropagation have also been created. The influence of cultivar, medium composition, explant disposition on the shoot and number of subcultivations on the propagation efficiency was shown. Based on the analysis of the regenerants from lavender, sage, geranium, coriander, yarrow and fennel calluses, the somaclonal variability by morphology and economic valuable characteristics have been determined, and prospects for breeding forms have been revealed. One of the practical results of these scientific studies is a new cultivar of clary sage Selinzh, which was created using somaclonal variants.

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Random Amplified Polymorphic DNA (RAPD) and Derived Techniques

Cited by 22 publications

References 92 publications

What Can Genetics Do for the Control of Infectious Diseases in Aquaculture?

What Can Genetics Do for the Control of Infectious Diseases in Aquaculture?

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Bionechnology of Essential Oil Plants: Creation of New Forms and Micropropagation in Vitro

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