Randomized, Controlled, Single-Blind Study on use of Autologous Keratinocytes on a Transfer Dressing to Treat Nonhealing Diabetic Ulcers

Abstract

Repeated regular applications of the patient's keratinocytes, delivered on the carrier dressing, initiated wound healing in ulcers resistant to conventional therapy, with 18 out of 21 ulcers responding. The healing observed did not appear attributable to patient recruitment or the cell-free carrier dressing but to the delivery of the cultured cells.

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

“…These cell carriers have been used successfully in the clinic to deliver autologous keratinocytes for the treatment of patients with extensive skin loss resulting from burn injury and diabetic foot ulcers. 20,[22][23][24] Here, we demonstrate for the first time that these carriers are supportive of MSC cultures. The cultures retain their phenotype and ability to support vascular tubule formation after 72 h culture on the carriers, and the carriers allow the effective transfer of MSCs to human dermis model wound beds.…”

“…Such dressings are currently being used in the clinic to deliver cultured autologous keratinocytes to patients with extensive burn injuries 23 and chronic wounds. 20,24 Given the potential therapeutic benefits that MSCs possess with respect to wound healing, it was of interest to determine whether similar surface preparations translate to the efficient delivery of these cells without altering the properties of the cells.…”

Development of biodegradable electrospun scaffolds for dermal replacement

“…While there have been significant advances in expanding skin cells and delivering them to patients for the treatment of extensive skin loss due to burns injury or chronic wounds [1][2][3][4][5] one major challenge which remains is the production of 3D tissue engineered skin which has both an epidermal and a dermal layer. In the treatment of extensive full thickness burns where sufficient autologous skin grafts are not immediately available for the patient the dermal and epidermal layers are often replaced in two separate operations (as described in Refs [6,7]).…”

Diabetes Mellitus and Regenerative Medicine: New Possibilities for the Regeneration of β Cells and Treatment of Diabetic Foot Ulcer

“…This is a health problem that affects a large number of individuals and the number of patients is on the increase [1]. There are two types of diabetes: type 1 diabetes (T1DM) is the first and it is characterized by an absolute insulin deficiency that is due to an immune-mediated destruction of β cells that are the production site of insulin within pancreatic islet of Langerhans.…”