Randomized research on the mechanism of local oxygen therapy promoting wound healing of diabetic foot based on RNA-seq technology

Wang, Shuo; Pan, Li-Feng; Gao, Lei; Qin, Xinyuan

doi:10.21037/apm-20-295

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…Diabetic foot ulcers (DFU) affect approximately 25% of diabetes patients (Li et al, 2021). When patients develop foot ulcers, they frequently face the risk of subsequent infection, amputation or even death (Wang, Pan, Gao, Qin, & Wang, 2021). An estimated 85% of them will require amputations (Boulton, 2019), and more than half of them will die within 5 years (Armstrong et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Sanguisorba officinalis L. promotes diabetic wound healing in rats through inflammation response mediated by macrophage

Song

Zeng

Zheng

et al. 2023

Phytotherapy Research

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Sanguisorba officinalis L., a traditional Chinese medicine, is frequently used to treat burns and scalds. But even so, it is unknown whether S. officinalis L. can accelerate diabetic wounds (DW) healing. Here, to bridge the gap, we employed in vivo and in vitro evaluations to assess the positive effect of S. officinalis L. ethanol extract (ESO) on DW. Results demonstrated that ESO dramatically improved the DW healing rate. With ESO treatment, the inappropriately elevated levels of IL6, IL1β and TNFα in DW were reduced, while the expression of IL10 was increased, indicating that the abnormal inflammation in DW was also under control. Moreover, the abnormally elevated expression of CD86 was significantly inhibited and the expression of CD206 was significantly up‐regulated following treatment with ESO. The global level of NF‐κB protein was not affected by ESO treatment, but it suppressed the expression of phosphorylated NF‐κB and prevented its nuclear entry. In addition, in RAW264.7 cells activated with lipopolysaccharide (LPS), the expression of NLRP3, Caspase1 and IL1β were significantly diminished following ESO treatment. In conclusion, ESO was proved to be a promising treatment for DW healing due to its potential to accelerate the healing process by suppressing the inflammatory response. This was achieved by increasing the ratio of M2 to M1 polarization through blocking the NF‐κB/NLRP3 signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Sanguisorba officinalis L. promotes diabetic wound healing in rats through inflammation response mediated by macrophage

Song

Zeng

Zheng

et al. 2023

Phytotherapy Research

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“…In addition to paying attention to the treatment of systemic factors, such as the control of blood sugar, blood pressure, blood lipid, anti-infection and the improvement of microcirculation, we must also pay attention to the standardized treatment of chronic wound itself. Only the comprehensive coordinated treatment of whole body and local can promote the healing of chronic difficult wound ( 22 , 23 ). DFU healing is a multi-step process that requires the coordination of 4 distinct but overlapping physiologically logical phases, including hemostasis, inflammation, proliferation, and remodeling ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Healing mechanism of diabetic foot ulcers using single-cell RNA-sequencing

Wang¹,

Wei²,

Li³

et al. 2023

Ann Transl Med

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Background: Diabetic foot ulcer (DFU) is one of the common and severe complications in diabetic patients, mainly caused by the interaction of various factors such as peripheral neuropathy, peripheral vascular disease, and infection. Moreover, vascular damage, disorder of tissue cells, decreased expression level of neurotrophic factor, and decreased growth factor caused by long-term exposure to a high glucose environment can also lead to prolonged or incomplete wound healing. This imposes a tremendous financial burden on the patients' family and society. Although various innovative techniques and drugs have been developed to treat DFU, the therapeutic effect is still unsatisfactory. Methods:We filtered and downloaded the single-cell dataset of diabetic patients from the Gene Expression Omnibus (GEO) website and used the Seurat package in R for creation of single-cell objects, integration, control of quality, clustering, cell type identification, differential gene analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and intercellular communication analysis.Results: Diabetic healing-related differentially expressed gene (DEG) analysis showed that there were 1,948 differential genes between tissue stem cells in healing and non-healing wounds, of which 1,198 genes were up-regulated and 685 genes were down-regulated. The results of GO functional enrichment analysis in tissue stem cells showed that they were closely related to wound healing. The CCL2-ACKR1 signaling pathway activity in tissue stem cells influenced the biological activity of endothelial cell subpopulation, which ultimately promoted the healing of DFU wounds. Conclusions:The CCL2-ACKR1 axis is closely associated with DFU healing.

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“…The other, a participant and outcome blind RCT 109 , which was assessed at low risk of bias, showed greater percentage wound closure in the topical oxygen group compared to sham therapy at 12 weeks. The remaining seven studies [113][114][115][116][117][118][119] were at high risk of bias as: neither participants nor outcome assessors were blind to the intervention allocation, [113][114][115][116][117][118][119] groups differed in baseline areas, studies reported no between group analysis, 115,117,118 or reported an absolute rather than relative area reduction 116 and thus any positive results reported were of low certainty.…”

Section: Outcome: Reduction In Ulcer Areamentioning

confidence: 99%

Effectiveness of interventions to enhance healing of chronic foot ulcers in diabetes: A systematic review

Chen,

Vilorio,

Dhatariya

et al. 2024

Diabetes Metabolism Res

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BackgroundIt is critical that interventions used to enhance the healing of chronic foot ulcers in diabetes are backed by high‐quality evidence and cost‐effectiveness. In previous years, the systematic review accompanying guidelines published by the International Working Group of the Diabetic Foot performed 4‐yearly updates of previous searches, including trials of prospective, cross‐sectional and case‐control design.AimsDue to a need to re‐evaluate older studies against newer standards of reporting and assessment of risk of bias, we performed a whole new search from conception, but limiting studies to randomised control trials only.Materials and MethodsFor this systematic review, we searched PubMed, Scopus and Web of Science databases for published studies on randomised control trials of interventions to enhance healing of diabetes‐related foot ulcers. We only included trials comparing interventions to standard of care. Two independent reviewers selected articles for inclusion and assessed relevant outcomes as well as methodological quality.ResultsThe literature search identified 22,250 articles, of which 262 were selected for full text review across 10 categories of interventions. Overall, the certainty of evidence for a majority of wound healing interventions was low or very low, with moderate evidence existing for two interventions (sucrose‐octasulfate and leucocyte, platelet and fibrin patch) and low quality evidence for a further four (hyperbaric oxygen, topical oxygen, placental derived products and negative pressure wound therapy). The majority of interventions had insufficient evidence.ConclusionOverall, the evidence to support any other intervention to enhance wound healing is lacking and further high‐quality randomised control trials are encouraged.

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Randomized research on the mechanism of local oxygen therapy promoting wound healing of diabetic foot based on RNA-seq technology

Cited by 6 publications

References 40 publications

Sanguisorba officinalis L. promotes diabetic wound healing in rats through inflammation response mediated by macrophage

Sanguisorba officinalis L. promotes diabetic wound healing in rats through inflammation response mediated by macrophage

Healing mechanism of diabetic foot ulcers using single-cell RNA-sequencing

Effectiveness of interventions to enhance healing of chronic foot ulcers in diabetes: A systematic review

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