Randomized trial and local biological effect of autologous platelets used as adjuvant therapy for chronic venous leg ulcers

Senet, Patricia; Bon, F.; Benbunan, M; Bussel, A; Traineau, R; Calvo, Fabien; Dubertret, Louis; Dosquet, Christine

doi:10.1016/s0741-5214(03)00908-x

Cited by 122 publications

(135 citation statements)

References 34 publications

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“…While the use of pooled blood is typically frowned upon in the United States, the use of allogenic PRP has been gaining popularity in a number of European studies with no mention of adverse immune reactions. [53][54][55][56] FIG. 12.…”

Section: 51mentioning

confidence: 99%

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Sell

Wolfe

Ericksen

et al. 2011

Tissue Engineering Part A

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Platelet-rich plasma (PRP) therapy has seen a recent spike in clinical interest due to the potential that the highly concentrated platelet solutions hold for stimulating tissue repair and regeneration. The aim of this study was to incorporate PRP into a number of electrospun materials to determine how growth factors are eluted from the structures, and what effect the presence of these factors has on enhancing electrospun scaffold bioactivity. PRP underwent a freeze-thaw-freeze process to lyse platelets, followed by lyophilization to create a powdered preparation rich in growth factors (PRGF), which was subsequently added to the electrospinning process. Release of protein from scaffolds over time was quantified, along with the quantification of human macrophage and adipose-derived stem cell (ADSC) chemotaxis and proliferation. Protein assays demonstrated a sustained release of protein from PRGF-containing scaffolds at up to 35 days in culture. Scaffold bioactivity was enhanced as ADSCs demonstrated increased proliferation in the presence of PRGF, whereas macrophages demonstrated increased chemotaxis to PRGF. In conclusion, the work performed in this study demonstrated that the incorporation of PRGF into electrospun structures has a significant positive influence on the bioactivity of the scaffolds, and may prove beneficial in a number of tissue engineering applications.

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Section: 51mentioning

confidence: 99%

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Sell

Wolfe

Ericksen

et al. 2011

Tissue Engineering Part A

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“…In both study groups, patients treated with platelet gels experienced quicker healing [44]. In contrast, there was no benefit from autologous frozen platelets in a case-controlled randomized study of chronic venous leg ulcers [45]. In those studies reporting successful treatments, platelet concentrations were 15-20 3 10 8 /ml [42,43 • ,44], whereas in that study reporting a lack of successful treatment, platelet concentration was 5 3 10 8 /ml [45].…”

Section: Nonmaxillofacial Studiesmentioning

confidence: 99%

Platelet gels and releasates

Borzini

Mazzucco

2005

Current Opinion in Hematology

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The preponderance of evidence indicates that platelets and their derivatives have the potential for a substantial therapeutic role in tissue regeneration. The results of recent research indicate that platelet-derived growth factors act in synergy with plasma-derived factors to activate a complex network of autocrine functions that modulate healing. Platelet-derivative products are promising therapeutics that offer new opportunities for research and applications of tissue engineering.

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“…Thirteen randomized clinical trials 25,[32][33][34][35][36][37][38][39][40][41][42][43][44] were also included in this review, six of which were double-blind placebo-controlled trials 25,[32][33][34][35][36] (Table 2). Regarding etiology, five of the studies recruited mixed-etiology ulcers, 25,32,36,38,41 three considered venous ulcers, 33,34,42 and the other five diabetic foot ulcers.…”

mentioning

confidence: 99%

“…Regarding etiology, five of the studies recruited mixed-etiology ulcers, 25,32,36,38,41 three considered venous ulcers, 33,34,42 and the other five diabetic foot ulcers. 36,37,39,40,44 Four studies, including mixed ulcers and venous ulcers, did not find clinical evidence to support the benefit of PRP in wound healing.…”

mentioning

confidence: 99%

“…36,37,39,40,44 Four studies, including mixed ulcers and venous ulcers, did not find clinical evidence to support the benefit of PRP in wound healing. [32][33][34][35] The largest study considering just venous ulcers was conducted by Stacey et al, 33 and included 86 patients (42 in the experimental and 44 in the control group). Autologous platelets were separated by centrifugation and platelet lysate was obtained by sonication.…”

mentioning

confidence: 99%

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Platelet-rich plasma for the treatment of chronic wounds: evidence to date

Conde-Montero¹,

Dobao²,

González³

2017

CWCMR

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Platelet-rich plasma (PRP) is a widely used throughout diverse fields of medicine for improving tissue regeneration. In dermatology, its main applications in the last few years have mainly been in antiaging treatment and wound healing. PRP contains platelets at higher concentrations than whole blood, and consequently represents a growth-factor pool. Chronic wounds are characterized by a prolonged inflammatory phase, involving a continuous destruction of matrix proteins and growth factors. Growth factors promote cell migration, proliferation, and differentiation, which are essential for wound healing. In this review, we explain the basic principles of PRP in wound healing and aim to offer an updated critical assessment of the available clinical evidence that supports the utility of PRP for the therapeutic challenge that chronic ulcers represent in our daily clinical practice.

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Randomized trial and local biological effect of autologous platelets used as adjuvant therapy for chronic venous leg ulcers

Abstract: Topical autologous platelets have no significant adjuvant effect on healing of chronic venous leg ulcers and increased wound fluid TIMP-1 concentration. Ulcer healing is associated with a decrease in wound fluid IL-8.

Cited by 122 publications

References 34 publications

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Incorporating Platelet-Rich Plasma into Electrospun Scaffolds for Tissue Engineering Applications

Platelet gels and releasates

Platelet-rich plasma for the treatment of chronic wounds: evidence to date

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