1997
DOI: 10.1139/m97-115
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Randomly amplified polymorphic DNA using the M13 core sequence of the vesicular–arbuscular mycorrhizal fungi Gigaspora margarita and Gigaspora gigantea

Abstract: Vesicular–arbuscular mycorrhizal (VAM) fungi are obligate symbionts, and a primary benefit provided to the host is the alleviation of stress. The recalcitrance of these fungi to grow in pure culture has spurred researchers to develop an alternative form of cultivation, namely the root organ culture (ROC). This synthetic form of production is new and efforts were made to use randomly amplified polymorphic DNA with the M13 minisatellite sequence as the polymerase chain reaction primer to look into polymorphism, … Show more

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Cited by 15 publications
(6 citation statements)
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“…As far as we know, these studies were always performed on isolates maintained as pure cultures in laboratory conditions (e.g. Gadkar et al. , 1997; Rosendahl & Taylor, 1997; Zézé et al.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…As far as we know, these studies were always performed on isolates maintained as pure cultures in laboratory conditions (e.g. Gadkar et al. , 1997; Rosendahl & Taylor, 1997; Zézé et al.…”
Section: Discussionmentioning
confidence: 99%
“…In order to study population diversity of AM fungi, DNA markers based on the polymerase chain reaction are promising tools as they require very small amounts of DNA. Recently, highly variable minisatellites (Gadkar et al. , 1997; Zézé et al.…”
Section: Introductionmentioning
confidence: 99%
“…Attempts were made to distinguish strains of AM fungi using dominant markers such as RAPD (Gadkar et al, 1997), ISSR ( Vandenkoornhuyse et al, 2001) and AFLP (Koch et al, 2004), and multi-locus co-dominant markers based on differences in intron size (Stukenbrock and Rosendahl, 2005a, b). However, surprisingly, the most powerful genetic markers for identification of individuals or clones of a given species, namely microsatellites, have not been described in AM fungi.…”
Section: Introductionmentioning
confidence: 99%
“…These include Acaulospora rehmii , Gigaspora rosea (Bago et al, 1998), Gigaspora margarita (Diop et al, 1992;MillerWideman and Watrud, 1984), Gigaspora gigantea (Gadkar et al, 1997), Glomus etunicatum (Schreiner and Koide, 1993), Glomus intraradices (Chabot et * FAX No: +86 10 62891016. E-mail: lixl@cau.edu.cn al., 1992;St-Arnaud et al, 1996), Glomus versiforme (Declerck et al, 1996;Diop et al, 1994), Glomus caledonium (Karandashov et al, 2000), Glomus fasciculatum and Glomus macrocarpum (Declerck et al, 1998), Glomus proliferum (Declerck et al, 2000), Glomus deserticola (Mathur and Vyas, 1995) and Glomus fistulosum (Gryndler et al, 1998).…”
Section: Introductionmentioning
confidence: 99%