Randomly amplified polymorphism detection (RAPD) reveals high genetic diversity in Thalassia testudinum banks ex König (Turtlegrass)

Kirsten, Janet H.; Dawes, Clinton J.; Cochrane, Bruce J.

doi:10.1016/s0304-3770(98)00070-9

Cited by 33 publications

(37 citation statements)

References 42 publications

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“…A study of genotypic variation in T. testudinum from southern Florida and Jamaica, using RAPD (random ampli®ed polymorphic DNA) by Kirsten et al (1998) found the observed partitioning of genetic variation was similarly distributed within populations ( 81%) as observed in this study ( 96%). These authors suggest that the low di erentiation of their sites can be explained by the long-distance movement of vegetative fragments (Kirsten et al 1998).…”

Section: Discussionsupporting

confidence: 75%

“…On a local scale, the relatively high genetic diversity within populations, observed by Kirsten et al (1998), may be attributable to rhizome extension, rather than long-distance vegetative propagules. Rhizome extension, which in T. is in a guerilla-like fashion (opportunistic spread with long internodes between short shoots), would result in a extremely mixed genotype population structure.…”

Section: Discussionmentioning

confidence: 95%

“…These authors suggest that the low di erentiation of their sites can be explained by the long-distance movement of vegetative fragments (Kirsten et al 1998). This would, in fact, be the only probable explanation for the occurrence of the same clone in Bermuda and Panama, as observed in this study.…”

Section: Discussionmentioning

confidence: 99%

“…To date, genetic diversity of seagrass populations has been assessed extensively in only a few species, with studies demonstrating a wide range in genetic variability from zero in Amphibolis antarctica to moderate in Thalassia testudinum (Kirsten et al 1998;Davis et al 1999) and Posidonia oceanica (Procaccini and Waycott 1998) to high in Posidonia australis (Waycott 1995;) and Zostera marina (Reusch et al 1998;Ruckelshaus 1998). Partitioning of variation within and between populations is often high (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Of particular interest has been the observation of die-o in T. testudinum meadows over large areas in Florida Bay, Florida, USA (Robblee et al 1991). This has led, in part, to the interest in population genetic studies of this species to determine if, perhaps, there is a genetic component to the turtle grass die-o (Kirsten et al 1998;Davis et al 1999). Our understanding of population genetic structure, the adaptive signi®cance of observed genetic diversity and the ability of seagrasses to recover from di erent types of disturbances is only beginning to develop (e.g.…”

Section: Introductionmentioning

confidence: 99%

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AFLP diversity within and between populations of the Caribbean seagrass Thalassia testudinum (Hydrocharitaceae)

Waycott

Barnes

2001

Marine Biology

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Genetic variation was assessed in the seagrass Thalassia testudinum from three regions of the Caribbean and north Atlantic using allozyme electrophoresis and ampli®ed fragment length polymorphism (AFLP) analysis. Very low allozyme variability was detected among the 196 shoots analyzed from a range of sites in the San Blas region of Panama. AFLP markers detected high similarity (0.87) among the population samples surveyed from Bermuda and Panama across six AFLP primer pairs and over 260 banding positions. High levels of gene¯ow were detected between all the sites analyzed (N m >1.7). Signi®cantly complete genotypic similarity was observed between samples from Bermuda and Panama, indicating that long distance vegetative fragment dispersal is highly probable. Very low genetic differentiation between all sites, even Bermuda and Panama, some 2,700 km apart, agrees with other studies and is further evidence of a highly uniform gene pool in T. testudinum. High levels of genetic uniformity in T. testudinum may be related to long-term environmental change over its geographic range. While AFLP analysis proved useful in determining genetic variation in this seagrass, the application of co-dominant markers such as microsatellites will be more informative in determining the nature of genetic uniformity and its adaptive signi®cance in T. testudinum.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

AFLP diversity within and between populations of the Caribbean seagrass Thalassia testudinum (Hydrocharitaceae)

Waycott

Barnes

2001

Marine Biology

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Pleistocene climate change and phylogeographic structure of the Gymnocarpos przewalskii (Caryophyllaceae) in the northwest China: Evidence from plastid DNA, ITS sequences, and Microsatellite

Jia

Zhang

2019

Ecology and Evolution

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Northwestern China has a wealth of endemic species, which has been hypothesized to be affected by the complex paleoclimatic and paleogeographic history during Quaternary. In this paper, we used Gymnocarpos przewalskii as a model to address the evolutionary history and current population genetic structure of species in northwestern China. We employed two chloroplast DNA fragments ( rps 16 and psb B‐ psb I), one nuclear DNA fragment (ITS), and simple sequence repeat (SSRs) to investigate the spatial genetic pattern of G. przewalskii . High genetic diversity (cpDNA: h S = 0.330, h T = 0.866; ITS: h S = 0.458, h T = 0.872) was identified in almost all populations, and most of the population have private haplotypes. Moreover, multimodal mismatch distributions were observed and estimates of Tajima's D and Fu's FS tests did not identify significantly departures from neutrality, indicating that recent expansion of G. przewalskii was rejected. Thus, we inferred that G. przewalskii survived generally in northwestern China during the Pleistocene. All data together support the genotypes of G. przewalskii into three groups, consistent with their respective geographical distributions in the western regions—Tarim Basin, the central regions—Hami Basin and Hexi Corridor, and the eastern regions—Alxa Desert and Wulate Prairie. Divergence among most lineages of G. przewalskii occurred in the Pleistocene, and the range of potential distributions is associated with glacial cycles. We concluded that climate oscillation during Pleistocene significantly affected the distribution of the species.

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The Biology of Thalassia: Paradigms and Recent Advances in Research

Tussenbroek¹,

Stapel²,

Erftemeijer³

Seagrasses: Biology, Ecology and Conservation

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Randomly amplified polymorphism detection (RAPD) reveals high genetic diversity in Thalassia testudinum banks ex König (Turtlegrass)

Cited by 33 publications

References 42 publications

AFLP diversity within and between populations of the Caribbean seagrass Thalassia testudinum (Hydrocharitaceae)

AFLP diversity within and between populations of the Caribbean seagrass Thalassia testudinum (Hydrocharitaceae)

Pleistocene climate change and phylogeographic structure of the Gymnocarpos przewalskii (Caryophyllaceae) in the northwest China: Evidence from plastid DNA, ITS sequences, and Microsatellite

The Biology of Thalassia: Paradigms and Recent Advances in Research

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