RANKL Expression in Periodontal Disease: Where Does RANKL Come from?

Chen, Bin; Wu, Wenlei; Sun, Weibin; Zhang, Qian; Yan, Fuhua; Xiao, Yin

doi:10.1155/2014/731039

Cited by 82 publications

(92 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It inhibits bone or tooth resorption by strongly binding to its ligand (RANKL), hence preventing the latter from binding to its receptor (RANK) (30).…”

Section: Discussionmentioning

confidence: 99%

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

et al. 2017

View full text Add to dashboard Cite

The aim of this study was to evaluate markers of bone loss and immune response present in evolution of periodontal disease. One hundred and two Wistar rats were divided into three animals groups: PD0, without ligation and PD15 days and PD60 days, submitted to ligation placement with a sterile 3-0 silk cord in the cervical region of the upper first molar on both sides. Samples were obtained from the gingival tissue for histomorphometric analysis, immunohistochemical analysis of RANK, RANKL, OPG, characterization of the inflammatory infiltrate, quantification of nitric oxide, MCP-1, RANTES, IP10 chemokines, and expression of the TGF-β1, VEG, and bFGF. The number of inflammatory cells in gingival tissue was higher in PD60 samples. The collagen content and the area occupied by birefringent collagen fibers were lower for PD60. Differential leukocyte counting showed that there was a significantly higher polymorphonuclear influx in group PD15, while PD60 showed a greater number of lymphocytes. PD60 showed higher RANTES, IP-10, MCP-1 gene transcripts, as well as a higher nitric oxide concentration. Clinical evaluation revealed that the PD60 group presented an increase in furcal area. In conclusion, in this animal model the increase of RANK/RANKL and HGF markers is related to a specific immune response, and probably contributed to the evolution of periodontal disease. Investigating the effect of these biomarkers can help in targeted therapy for bone resorption, since blocking these can inhibit bone loss.

show abstract

“…It inhibits bone or tooth resorption by strongly binding to its ligand (RANKL), hence preventing the latter from binding to its receptor (RANK) (30).…”

Section: Discussionmentioning

confidence: 99%

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Based on previous studies that reported the stimulatory effect of IL-18 on GM-CSF expression in CD4 + T cells [11][12][13] , we hypothesized that the free IL-18BP in culture supernatants derived from RANKL-treated RAW264. An increase in the level of IL-18 has been observed in the serum or pathological tissue in individuals with rheumatoid arthritis [28][29][30][31] or periodontitis 32,33) , both of which are well-recognized as inflammatory diseases with bone destruction, through RANKLinduced osteoclast formation [34][35][36] . The results in our present study may help explain how RANKL, IL-18 and IL-18BP are involved in the cell-cell interactions between osteoclast precursor cells and CD4 + T cells in these bone-destructive disorders.…”

Section: Effect Of Conditioned Medium Derived From Raw2647 Cells On mentioning

confidence: 99%

RANKL Induces IL-18 Binding Protein Expression in RAW264.7 Cells

Takahashi

Tanaka

Nakai

et al. 2016

J. Hard Tissue Biology.

View full text Add to dashboard Cite

The receptor activator of NF-B (RANK) ligand (RANKL) is a cytokine that is essential for osteoclast development, whereas interleukin (IL)-18 suppresses osteoclastogenesis by increasing granulocyte-macrophage colony-stimulating factor (GM-CSF) production in T-cells. In the present study, we examined the effect of RANKL on the expression of IL-18 and IL-18 binding protein (IL-18BP), a natural inhibitor of IL-18, in RAW264.7 cells. We also examined the effect of conditioned medium derived from RAW264.7 cells on IL-18-induced GM-CSF expression in CD4 + T cells isolated from the spleens of C57BL/6J mice. mRNA expression of IL-18 was significantly suppressed, whereas that of IL-18BP was significantly increased in RANKL-treated RAW264.7 cells compared with untreated cells. RANKL also increased the expression of IL-18BP protein in culture supernatants of RAW264.7 cells. GM-CSF protein expression in CD4 + T-cells stimulated with IL-18 was suppressed by the addition of conditioned medium derived from RANKL-treated RAW264.7 cells. These results suggest that RANKL suppresses the stimulatory effect of IL-18 on GM-CSF expression in CD4 + T-cells via enhancing the production of IL-18BP in RAW264.7 cells.

show abstract

“…Possui importante papel na regulação da osteoclastogênese devido a sua ação na expressão de RANK-L (CHEN et al, 2014).…”

Section: Fator De Necrose Tumoral-α (Tnf-α)unclassified

“…O nível de RNA-m de RANKL está aumentado em processos inflamatórios como da DP. As citocinas IL-1β e TNF-α podem regular a expressão de RANKL em células periodontais e aumentar a formação de osteoclastos (CHEN et al, 2014).…”

Section: Ligante Do Receptor Ativador De Nf-kb (Rankl)unclassified

“…Provavelmente a quantidade de OPG não foi suficiente para diminuir a progressão da perda óssea, ao passo que a resposta imune contra patógenos periodontais pode afetar grandemente o curso da DP e a perda óssea continua a ser estabelecida (CHEN et al, 2014;NAGASAWA et al, 2000). Entretanto um estudo utilizando extrato de sumagre (antioxidante, anti-inflamatório, antimicrobiano) em ratos após indução de doença periodontal experimental, houve aumento de células do infiltrado inflamatório, osteoclastos e RANKL no grupo submetido a ligadura sem tratamento e presença de mais células de OPG no grupo com ligadura e tratado com o extrato (SAGLAM et al, 2015) Em suma, os resultados dessa pesquisa mostraram que ramipril foi capaz de diminuir a progressão da perda óssea na doença periodontal induzida experimentalmente em ratos no grupos tratadocom ramipril DP-21d-Rami.…”

Section: Osteoprotegerina (Opg)unclassified

See 1 more Smart Citation

Efeitos do ramipril sobre a doença periodontal induzida experimentalmente em ratos

Akashi¹

View full text Add to dashboard Cite

RANKL Expression in Periodontal Disease: Where Does RANKL Come from?

Cited by 82 publications

References 61 publications

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

RANKL Induces IL-18 Binding Protein Expression in RAW264.7 Cells

Efeitos do ramipril sobre a doença periodontal induzida experimentalmente em ratos

Contact Info

Product

Resources

About