RANTES Augments Radical Oxygen Products from Eosinophils

Abstract

RANTES, which is released from thrombin-stimulated platelets, is a member of the 8-kD cytokine family that has been shown to possess chemotactic activity for eosinophils. Thus, in this study, we examined the effect of RANTES on radical oxygen products from eosinophils. RANTES treatment resulted in the enhancement of peak value and intergrated value of productivity of eosinophil-mediated radical oxygen products determined by luminol-dependent chemiluminescence of EoL-3 cells stimulated with A23187. In addition, the radical oxygen products of EoL-1 or eosinophils showed similar results. Thus, we could conclude that platelets might play an important role in the pathogenesis of allergic inflammation through the involvement in the selective eosinophil infiltration and eosinophil activation by releasing RANTES.

Functional Expression and Characterization of Macaque C-C Chemokine Receptor 3 (CCR3) and Generation of Potent Antagonistic Anti-macaque CCR3 Monoclonal Antibodies

“…These biological effects include calcium mobilization, chemotaxis, integrin up-regulation, degranulation, and transendothelial migration in vitro (72)(73)(74)(75)(76)(77). In addition, intradermal injection of human RANTES into allergic human subjects was shown to cause a rich eosinophil recruitment into the skin in vivo (78).…”

Role of Chemokines in Glomerular Diseases

“…Using immunohistology and in situ hybridization, ex pression of MI P-1 P and RANTES was detected in rejecting renal allografts [85][86][87]. Both chemokines were expressed by mononuclear cells and tubular epithelium.…”

Possible Presence of RANTES in Tears of Patients with Allergic Conjunctivitis

“…These findings suggest that RANTES might play a key role in the invol vement of eosinophils and T cells in allergic conjunctivitis. We recently examined the ef fect o f RANTES on radical oxygen products from eosinophils [6]. RANTES treatment re sulted in the enhancement o f peak value and integrated value of radical oxygen species generated by eosinophils determined by luminol-dependent chemiluminescence [6], Therefore, RANTES might play a major role in the pathogenesis of allergic inflammation, since RANTES not only attracts eosinophils to the inflamed site [3] but also up-regulates the expression of CR3 (Mac-1:CDI lb), caus es the release of eosinophil cationic proteins, makes eosinophils hypodense [7], and aug ments the oxygen radical production from eo sinophils as we previously reported [6], In this context, it is a great interest to clarify the source o f RANTES production in the patho genesis o f allergic conjunctivitis.…”