Rapamycin ameliorates corneal injury after alkali burn through methylation modification in mouse TSC1 and mTOR genes

Li, Jiande; Du, Shanyi; Shi, Yu; Han, Jiangyuan; Niu, Zhanyu; Li, Wei; Yang, Pengfei; Chen, Linchi; Tian, Huanbing; Gao, Lan

doi:10.1016/j.exer.2020.108399

Cited by 18 publications

(20 citation statements)

References 34 publications

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“…2 However, clinically available agents have poor treatment effects on corneal alkali burns, especially corneal opacity and NV development. 3 No agent has been approved for the treatment of corneal NV to date. 4 Therefore, the treatment of ocular chemical burns is still an area of investigation.…”

Section: Introductionmentioning

confidence: 99%

A simple but novel glycymicelle ophthalmic solution based on two approved drugs empagliflozin and glycyrrhizin: in vitro/in vivo experimental evaluation for the treatment of corneal alkali burns

Zong-jian

Zhang

Wei³

et al. 2023

Biomater. Sci.

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Section: Introductionmentioning

confidence: 99%

A simple but novel glycymicelle ophthalmic solution based on two approved drugs empagliflozin and glycyrrhizin: in vitro/in vivo experimental evaluation for the treatment of corneal alkali burns

Zong-jian

Zhang

Wei³

et al. 2023

Biomater. Sci.

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“…( 1989 ), corneal alkali burn injury model has been used especially in rodents and rabbits to study the pathophysiology of corneal burn injuries and also for developing novel therapeutic approaches (Hao & Nourbakhsh, 2021 ). This model is also used for evaluation of the effects of different agents such as doxycycline hyclate (Perry et al., 1993 ), dexamethasone (Chung et al., 1999 ) and more recently rapamycin (Li et al., 2021 ) among others on corneal ulcer healing.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of delaying effects of different short‐term dosage regimens of topical ciprofloxacin on corneal ulcer healing in an avian model

Tavangarrad

Mosleh

Shomali

et al. 2022

Veterinary Medicine & Sci

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Background: Knowledge on possible delaying effects of topical ciprofloxacin on corneal ulcer healing is scarce in avian patients. Objectives:The study evaluates effects of different dosage regimens of topical ciprofloxacin on healing of corneal ulcer in an avian model. Methods:One hundred and fifty adult layers were randomly allocated into two equal categories each consisted of 5 groups (n = 15): 1, negative control (NC, normal cornea); 2, positive control (PC) (birds with experimental corneal ulcer); and 3, 4 and 5, birds with corneal injury that received ciprofloxacin 0.3% topically q6h, q8h and q12h, respectively for 3 (category 1) or 5 days (category 2). Corneas were excised for histopathological evaluation and determination of MMP-9 expression.Results: While no significant difference was observed in daily-measured fluoresceinstained ulcer size among ciprofloxacin-treated birds and PC group in category 1, birds in PC group of category 2 had significantly smaller ulcers as compared to antibiotictreated birds at the end of experiment (p < 0.01 for all cases). Histopathological evaluations at the end of the experiment showed no significant difference among PC and ciprofloxacin-treated birds of both categories for almost all of the assayed parameters. Over expression of MMP-9 mRNA was observed in PC group after 3 and 5 days of ulcer induction compared to NC groups. Its expression in ciprofloxacin-treated birds of both categories remained close to PC groups.Conclusions: While ciprofloxacin administration for 3 days does not affect ulcer healing, it delays healing process at the end of 5 days of treatments in an avian model of corneal ulcer injury. This delaying effect is not associated with a drastic change in MMP-9 expression.

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“…[6] Li et al showed that rapamycin was effective in repairing corneal damage caused by alkali burn in mouse TSC1 and mTOR genes. [7] In addition, Husain and Byrareddy recommend low doses of rapamycin for the potential treatment of Coronaviruses Disease 2019/Severe Acute Respiratory Syndrome Coronavirus 2 infection, due to its ability to inhibit protein synthesis and the activities or expression of pro-inflammatory cytokines, as well as targeting host factors, not the viral mechanism. [8] Since drug analysis is very important in the quality control of drug formulations, qualitative/quantitative analysis of active ingredients in drugs should be made in a simple, precise, and accurate manner.…”

Section: Introductionmentioning

confidence: 99%

Development of a voltammetric method for the determination of rapamycin in pharmaceutical samples at pretreated pencil graphite electrode

Karaboduk

2021

J Chinese Chemical Soc

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This paper describes an electroanalytical method for the determination of rapamycin using a pretreated pencil graphite electrode (P‐PGE) by differential pulse voltammetry. The electrodes were successfully characterized by cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, and Fourier transform infrared. The influences of instrumental and chemical parameters were investigated and optimized for the determination of rapamycin. Oxidation peak current obtained for rapamycin at P‐PGE was 10.5 times higher than that obtained for PGE. In pH = 3.0 H3PO4, a well‐defined irreversible anodic peak occurred at 1.07 V, which was attributed to the electro‐oxidation of rapamycin at P‐PGE. Under optimized conditions, the oxidation peak current of rapamycin was linear with its concentration in the range of 0.01–250 μmol/L, and the detection limit was 0.0075 μmol/L (S/N = 3). Also, the P‐PGE exhibited good reproducibility (relative SD [RSD] = 2.17% for 10 electrodes at different times using the same procedure), repeatability (RSD = 2.09% for 10 measurements with same electrode), and stability (95.80% for 10 days). The P‐PGE was simple to prepare and the method was successfully applied for the determination of rapamycin in spiked and real samples. In addition, for comparison, high‐performance liquid chromatographic method with ultraviolet (UV) detector was used for the determination of rapamycin.

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Rapamycin ameliorates corneal injury after alkali burn through methylation modification in mouse TSC1 and mTOR genes

Cited by 18 publications

References 34 publications

A simple but novel glycymicelle ophthalmic solution based on two approved drugs empagliflozin and glycyrrhizin: in vitro/in vivo experimental evaluation for the treatment of corneal alkali burns

A simple but novel glycymicelle ophthalmic solution based on two approved drugs empagliflozin and glycyrrhizin: in vitro/in vivo experimental evaluation for the treatment of corneal alkali burns

Evaluation of delaying effects of different short‐term dosage regimens of topical ciprofloxacin on corneal ulcer healing in an avian model

Development of a voltammetric method for the determination of rapamycin in pharmaceutical samples at pretreated pencil graphite electrode

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