RAPD analysis of genetic diversity in natural populations of Aegilops geniculata Roth and Triticum durum Desf from Tunisia

Mahjoub, Asma; Mguis, Kaled

doi:10.5251/abjna.2012.3.11.466.475

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…Genetic diversity was high within populations or germplasm types but relatively low between populations. These findings are in accordance with a number of studies (Arora et al 2014;Dashchi et al 2012;Mahjoub et al 2012). The low variability among populations can be explained by the high gene flow value obtained, which is a general indicator of the magnitude of genetic exchange (Abouzied et al 2013), the level of differentiation among the population being inversely proportional to the value of gene flow.…”

Section: Discussionsupporting

confidence: 92%

Validation of simple sequence repeats associated with quality traits in durum wheat

AMALLAH

Taghouti

Rhrib

et al. 2016

J. Crop Sci. Biotechnol.

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Over recent years, quality has become an important commercial issue for durum wheat breeders. Modern breeding methods are most efficient for producing and supplying the best quality raw materials to the pasta industry. Here we assessed the effectiveness of molecular marker-assisted selection of quality traits in durum wheat. To this end, DNA and quality trait markers were jointly used to analyze quality-related traits in a durum wheat collection. A total of 132 durum wheat (Triticum turgidum ssp. durum) Mediterranean landraces, international lines, and Moroccan cultivars were analyzed for seven important qualityrelated traits including thousand-kernel weight (TKW), test weight (TW), gluten strength, yellow pigment (YP), and grain protein content (GPC). Additionally, 18 simple sequence repeat (SSR) markers previously reported to be associated with different quality traits were analyzed. Of these, 14 (78%) were polymorphic and four were monomorphic. There were between two and seven alleles per locus, with an average of four alleles per locus. The average phenotypic variation value (R 2 ) ranged from 2.81 to 20.43%. Association analysis identified nine markers significantly associated with TKW, TW, and YP, followed by eight markers associated with GPC, six markers associated with yellow index b, four markers associated with brightness L, and three markers associated with SDS-sedimentation volume. This study highlights the efficiency of SSR technology, which holds promise for a wide range of applications in marker-assisted wheat breeding programs.

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Section: Discussionsupporting

confidence: 92%

Validation of simple sequence repeats associated with quality traits in durum wheat

AMALLAH

Taghouti

Rhrib

et al. 2016

J. Crop Sci. Biotechnol.

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“…Such a result is expected with regard to the autogamous breeding system of B. distachyon s.l and confirms the lack of inter-crossing pollination (Vogel et al 2009). This finding does not agree with that presented by Filiz et al (2009) using AFLP markers and Turkish natural populations of B. distachyon but is in accordance with that found by Mahjoub et al (2012) in Tunisian natural populations of Aegilops geniculata Roth (Poaceae) using RAPD markers (0·80 of within-population variation) and Ehtemam et al . (2009) in Triticum species in Iran.…”

Section: Discussioncontrasting

confidence: 59%

Morpho-phenological diversity among Tunisian natural populations ofBrachypodium hybridum

et al. 2014

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Brachypodium hybridum belongs to the Poaceae grass subfamily. It has a close genetic relationship with temperate cereal crops, which means that it can be used as a model for temperate cereal and grass crops. In order to improve knowledge on the genetic diversity of this species, 145 lines of B. hybridum representative of nine populations and all the ecoregions of Tunisia were characterized on the basis of 18 morpho-phenologic features. The results show a considerable variation between populations and ecoregions in all traits studied. Variation was relatively higher for reproductive than vegetative traits. The majority of traits showed very low to high heritability with low border value for average length of spikelet (ALS) and an average value of 0·64. It is noticeable that high values of heritability were observed for most vegetative descriptors, with low values for reproductive ones. Differentiation between populations (Q ST ) varied from 0·02 for ALS to 0·78 for average length of leaves with a mean value across traits of 0·4, which confirms the wide intra-population variation in Tunisian natural population of B. hybridum. Pairwise Q ST showed that the greatest differentiation among populations was registered between Ain Drahem and Jbel Zaghouan and the smallest between Haouria and Raoued. Overall, the Ain Draham population showed the largest differentiation from the rest of the populations. To infer the effect of geographic distribution of the species, a Mantel test was applied between observed pairwise differentiation and geographic distance between populations and between ecoregions: the results show a positive, but not significant, relationship. In addition a significant negative relationship was found between phenotypic diversity and altitude, indicating that genetic diversity decreased with increasing altitude. Taken together, the high levels of intrapopulation variation and the lack of correlation between genetic differentiation and geographic distribution suggest a potentially important rate of long-distance seed dispersal and confirm the role played by natural selection in the population structure of Tunisian natural populations of B. hybridum.

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“…The results of AMOVA, based on SSR markers, revealed a significantly greater within species genetic variation than between species (Table 3). Likewise, a previous study on Aegilops geniculata and Triticum durum showed a greater within species genetic variation than between species (MAHJOUB et al 2012).…”

Section: Discussionmentioning

confidence: 61%

Genetic diversity of wheat wild relatives using SSR markers

et al. 2018

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Wild relatives of wheat are potential sources of valuable genetic materials for wheat improvement. Knowledge of the genetic diversity of wild relative species of wheat is crucial for their conservation and utilization. The objective of the current study was to investigate the genetic diversity of inter and intra species of Triticum monococcum ssp. aegilopoides (AA), Aegilops tauschii (DD) and Aegilops cylindrica (CCDD) originating from northern and western Iran. Thirty microsatellite (SSR) markers belonging to A, B, C and D genomes were used for analysis and 20 found to be polymorphic within and between species. The SSR markers generated a total number of 180 alleles with an average of 9 alleles per locus in 21 genotypes. The genetic diversity for all loci ranged from 0.74-0.90 with an average of 0.83. The highest genetic diversity was estimated for Xgwm186 and Xgwm205 which the latter could amplify in the A, D and CD genomes of T. monococcum, Ae. tauschii and Ae. cylindrica, respectively. In addition, the number of bands generated by Xgwm205 along with other four markers in Ae. cylindrica (CD) was twofold than that of Ae. tauschii (D). Polymorphic information content ranged from 0.7-0.89 with an average of 0.82. The dendrogram obtained from the neighbor-joining method divided the genotypes of the three species into three distinctive groups. It can be concluded that SSR markers can be useful not only in differentiating wild species of wheat possessing A, D and C genomes, but also in assessing the genetic variation of genotypes within these species.

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RAPD analysis of genetic diversity in natural populations of Aegilops geniculata Roth and Triticum durum Desf from Tunisia

Cited by 4 publications

References 32 publications

Validation of simple sequence repeats associated with quality traits in durum wheat

Validation of simple sequence repeats associated with quality traits in durum wheat

Morpho-phenological diversity among Tunisian natural populations ofBrachypodium hybridum

Genetic diversity of wheat wild relatives using SSR markers

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