RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey

Abstract

Bayazit S., Imrak B., Küden A., Kemal Güngör M., 2011. RAPD analysis of genetic relatedness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Turkey. Hort. Sci. (Prague), 38: 134-141.Quince (Cydonia oblonga Mill.) is a minor fruit crop, which is primarily used for marmalade, jam, sauce and as rootstocks for pears. Different cultivated and local quince genotypes are grown in almost all parts of Turkey for fruit usage. In this study, randomly amplified polymorphic DNA (RAPD) technology was used to study the genetic relationships among 13 quince accessions selected from different parts of Turkey. Thirty decamer primers were used and 14 of them did not produce any polymorphism. The remaining 16 primers ranged in their amplification fragments between one (P-402, P-437, OPA 10, OPA 16, OPA 18 and OPA-19) and five (OPA-06 and OPA-07). The size of fragments varied from 100 to 1500 bp. Similarity values among the studied genotypes ranged between 0.483 and 0.925. The resulting dendrogram clustered into two groups (0.69 similarity value) based on evaluation of genetic similarities and differences. The results suggest that RAPD analysis could be used to distinguish and determine genetic variation among quince accessions. Also, the obtained clustering based on RAPD markers agreed to some extent with the geographical origin of the studied set of quince accessions.

Characterization of Anatolian traditional quince cultivars, based on microsatellite markers

“…Similarity ratios between 'Limon-Kalecik' and 'Limon-İstanbul' cultivars that were based on RAPD markers used in a previous study (Bayazit et al, 2011) were similar with those obtained in the present study. The similarity ratio between Kalecik-Osmancık was found to be the same (62%) in both studies.…”

“…from Turkey have been genetically characterized with SSR markers and other molecular markers (Ayanoğlu et al, 2007;Akpınar et al, 2010;Bayazit et al, 2011;Yilmaz et al, 2011), no SSR-based genetic identification study has been conducted on Turkish quince germplasm without clonal variation study of Kalecik quince cultivar by Dumanoğlu et al (2009). The findings of this study will…”

“…Eleven groups of quince and two groups of X pyronia (quince-pear crosses) were distinguished by isozyme patterns such as acid phosphatase, esterase, peroxidase, and phenol oxidase. As a dominant marker, random amplified polymorphic DNA (RAPD) was used (Bayazit et al, 2011) for determining genetic relationships among 13 traditional quince accessions that were selected from different parts of Turkey.…”

Genetic variation among quince (Cydonia oblonga Mill.) genotypes sampled from the Coruh valley in Turkey

“…A 30-mL reaction cocktail was prepared as follows: 10X 3.0 mL buffer, 1.2 mL 10 mM dNTPs, 1.2 mL 25 mM magnesium chloride, 2.0 mL 5 mM primer, 0.4 mL 5 U Taq polymerase, 19.2 mL water, and 3.0 mL 100 ng/mL sample DNA. A total of 10 RAPD primers, which were previously efficiently used in quince (Bayazit et al, 2011), were tested in this study (Table 1).…”

“…In Turkey, only a few molecular genetic studies have been conducted on quince to date (Dumanoglu et al, 2009;Bayazit et al, 2011). Moreover, no studies have previously considered molecular genetic characteristics of quince grown in the northeastern part of Turkey.…”