RAPD analysis of somaclonal variants derived from embryo callus cultures of peach

Hashmi, Ghazala; Huettel, R. N.; Meyer, Rhonda C.; Krusberg, L. R.; Hammerschlag, F. A.

doi:10.1007/s002990050290

Cited by 57 publications

(16 citation statements)

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“…Rani et al (1995) observed polymorphic bands in six of 23 micropropagated plants of Populus deltoides. Munthali et al (1996) and Hashmi et al (1997), also reported polymorphisms in micropropagated plants of Beta vulgaris and Prunus persica, respectively. Similarly, Hofmann et al (2004) reported RAPD band polymorphism in embryogenic cultures of soybean that were treated with ethyl methanesulfonate (EMS).…”

Section: Discussionmentioning

confidence: 85%

Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica

Feyissa¹,

Welander²,

Negash³

2007

Biologia plant.

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Randomly amplified polymorphic DNA (RAPD) markers were used to assess genetic stability of 80 micropropagated Hagenia abyssinica plants, 40 of axillary origin and 40 of adventitious origin. The shoots were isolated from the same mother tree and micropropagated for over two years. Among the 83 RAPD primers screened, 16 gave reproducible band patterns. These 16 primers produced 115 bands for each plant. One plant from axillary origin showed two unique bands with primer OPC-11. All other plants showed identical band patterns. Generally, there was no significant difference in the shoot multiplication rate between shoots of axillary and adventitious origin. Indole-3-acetic acid (IAA) resulted in better ex vitro rooting compared to indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA). Nonmicropropagated plants that were grown in the greenhouse for about one year were better in ex vitro rooting compared to those of juvenile material and mature tree derived micropropagated plants of the same treatment. Adventitious rooting related oxygenase gene (ARRO-1) isolated from apple (Malus domestica) was not expressed in H. abyssinica using a complementary DNA representational difference analysis fragment (cDNA RDA14) as a probe.

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Section: Discussionmentioning

confidence: 85%

Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica

Feyissa¹,

Welander²,

Negash³

2007

Biologia plant.

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“…In order to evaluate the effects of in vitro culture on the genetic integrity of micropropagated kiwifruit, we used two different molecular markers: RAPDs and SSRs. While RAPD markers are extensively used to assess genetic variation generated by in vitro techniques (Hashmi et al 1997;Goto et al 1998), SSRs are relatively recent molecular markers that have been mainly used in human and animal genetics (Weber 1990) and, more recently, in such plants as lettuce ( Van der Wiel et al 1998), grape (Thomas and Scott 1993), wheat (Plashke et al 1995 and Pelargonium spp. (Becher et al 2000) to measure genetic similarity (or dissimilarity).…”

Section: Introductionmentioning

confidence: 99%

“…Various DNA markers have been developed, such as RFLP (restriction fragment length polymorphism), AFLP (amplified fragment length polymorphism), RAPD (random amplified polymorphic DNA) and microsatellites (SSRs, simple sequence repeats). A few of these have been used to assess genetic variation induced by tissue culture in plants (Sabir et al 1992;Isabel et al 1993;Schneider et al 1996), including fruit trees (Rani et al 1995;Hashmi et al 1997;Goto et al 1998;Vendrame et al 1999). In order to evaluate the effects of in vitro culture on the genetic integrity of micropropagated kiwifruit, we used two different molecular markers: RAPDs and SSRs.…”

Section: Introductionmentioning

confidence: 99%

Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev)

2002

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Two different DNA-based techniques, random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers, were used for fingerprinting kiwifruit genotypes and for detecting undesirable genetic variation in micropropagated plants. The fragments were scored as present (1) or absent (0), and those readings were entered in a computer file as a binary matrix (one for each marker). Two cluster analyses were performed to express -in the form of dendrograms -the relationships among the genotypes and the genetic variability detected. Both DNA-based techniques were able to amplify all of the genotypes, but only SSR markers could detect genetic variation induced in micropropagated plants of cv. Tomuri. Two hypotheses were formulated to explain these results, both of them are in agreement with the results obtained using these two types of molecular markers. We conclude that when the tissue culture technique is used, the analysis of somaclonal variability could require more than one DNA-based technique; in fact, the genetic variation present in different sources could interfere or combine with the more or less polymorphic ability, as our results showed for SSR and RAPD markers.

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“…However, except few fragmentary reports on micropropagation using various explants and strategy (Kalia et al 2004;Nurul Islam and Rahman 2005), there is no report on somatic embryogenesis in B. nutans as employed in the present study. Further, considering the importance of genetic uniformity during somatic embryogenesis to characterize somaclonal variation with greater precision as reported in earlier tissue culture studies in different plant species (Cloutier and Landry 1994;Hashmi et al 1997), amplified fragment length polymorphism (AFLP) fingerprinting was employed to confirm the genetic stability. High levels of reproducibility coupled with high multiplex ratio over the other molecular markers techniques (Powell et al 1996) are the reasons for selecting AFLP markers for evaluating genetic fidelity of tissue culture-raised plants in this study.…”

Section: Introductionmentioning

confidence: 99%

Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers

et al. 2010

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Bambusa nutans Wall., is an evergreen, perennial, and multipurpose bamboo having strong culms, which are largely used for construction, scaffolding, craft purposes, pulp, and paper industry. Multiple shoots from nodal segments (3-4 cm) of young branches of mature culms were established in Murashige and Skoog (1962) (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) (1.0-6.0 mg l -1 ) or in combination with a-naphthaleneacetic acid (NAA) (0.5-1.0 mg l -1 ) or kinetin (Kn) (1.0-2.0 mg l -1 ). February-March and December were found to be the best seasons for culture establishments. Maximum shoots were achieved on MS medium fortified with BAP (2.0 mg l -1 ). Embryogenic callus (slightly greenish compact, globular, and slow growing) was initiated from the base of severed sprouted buds in 2-3 subsequent subcultures on MS medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (5.0 mg l -1 ) under dark incubations. Maturation and germination of well-organized somatic embryos was achieved on MS medium containing BAP and 2,4-D (1.0 mg l -1 each) with 20.0 mg l -1 ascorbic acid. Fullstrength MS medium supplemented with 2% glucose favored further development of proliferated somatic embryos into plantlets. Genetic variations of fieldestablished B. nutans plants regenerated through tissue cultures were assessed by amplified fragment length polymorphism (AFLP) analysis using 6 primer combinations. Four hundred and seven scorable fragments were amplified, of which 402 (98.8%) have recorded conservation at various morphogenetic stages leading to plantlets regeneration, therefore, revealed a high level of genetic stability.

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RAPD analysis of somaclonal variants derived from embryo callus cultures of peach

Cited by 57 publications

References 0 publications

Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica

Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica

Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev)

Induction of somatic embryogenesis and analysis of genetic fidelity of in vitro-derived plantlets of Bambusa nutans Wall., using AFLP markers

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