RAPD analysis of southern populations of Gammarus locusta: comparison with allozyme data and ecological inferences

Costa, Filipe O.; Neuparth, Teresa; Theodorakis, Christopher W.; Costa, Maria Helena; Shugart, Lee R.

doi:10.3354/meps277197

Cited by 15 publications

(8 citation statements)

References 51 publications

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“…High connectivity along the western coast of Portugal had already been described for the amphipod, Gammarus locusta (Costa et al 2004), as well as for the seaweed Gelidium sesquipedale (Alberto et al 1999). For G. locusta, a unidirectional southeast migration between the western coast and the western tip of the southern coast was also detected.…”

Section: Discussionmentioning

confidence: 82%

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Invasion or invisibility: using genetic and distributional data to investigate the alien or indigenous status of the Atlantic populations of the peracarid isopod, Stenosoma nadejda (Rezig 1989)

et al. 2009

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The peracarid isopod, Stenosoma nadejda (Rezig 1989), until recently considered to be endemic of the Mediterranean region, was first reported in the Atlantic coast of southern Spain in 2001, and in 2006 abundant populations were discovered throughout the southwestern Portuguese coast. This fast expansion was intriguing because, as a direct brooder, this species has limited mechanisms for dispersal, such as rafting on seaweeds. Did S. nadejda recently extend its range into the Atlantic or was it overlooked in the past? We examined the patterns of genetic diversity and population differentiation accordingly by sequencing the cytochrome c oxidase subunit I mitochondrial gene from 75 individuals collected in five locations in Atlantic Iberia and one in the Mediterranean. Our results indicate that the newly discovered Atlantic populations of S. nadejda appear to be old and have long persisted on Atlantic shores rather than being a recent introduction. High levels of genetic diversity and geographic structure were uncovered in what was initially suspected to be an 'invasive' species. Recent changes in population dynamics may have made S. nadejda more conspicuous in the Atlantic shores, or a more comprehensive survey led to the recognition of this species where it was not expected.

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Section: Discussionmentioning

confidence: 82%

“…High connectivity along the western coast of Portugal had already been described for the amphipod, Gammarus locusta (Costa et al. 2004), as well as for the seaweed Gelidium sesquipedale (Alberto et al.…”

Section: Discussionmentioning

confidence: 84%

Invasion or invisibility: using genetic and distributional data to investigate the alien or indigenous status of the Atlantic populations of the peracarid isopod, Stenosoma nadejda (Rezig 1989)

et al. 2009

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“…At the time of collection, G. locusta could not be found on the Isle of Skye probably because this species is outcompeted by G. oceanicus and has migrated into deeper water [33], [34]. Similarly, G. locusta was not found on costal sites close to Tromsø because this species is unlikely to be found so far north [33].…”

Section: Discussionmentioning

confidence: 99%

“…G. locusta has a more southerly distribution extending from Norway to southern Portugal and Spain [33]. Further south G. locusta occupies the low intertidal, but further north along Norwegian coasts, G. locusta occupies the most exposed parts of the outer fjords and may even become sublittoral to avoid competition with G. oceanicus [33], [34]. G. locusta has not been reported in northern Norway [34], but G. oceanicus, G. locusta and G. duebeni are all found in the Baltic Sea where salinities are low but stable [35].…”

Section: Methodsmentioning

confidence: 99%

Influence of Natural Thermal Gradients on Whole Animal Rates of Protein Synthesis in Marine Gammarid Amphipods

Rastrick

Whiteley

2013

PLoS ONE

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Although temperature is known to have an important effect on protein synthesis rates and growth in aquatic ectotherms held in the laboratory, little is known about the effects of thermal gradients on natural populations in the field. To address this issue we determined whole-animal fractional rates of protein synthesis (ks) in four dominant species of gammarid amphipods with different distributions along the coasts of Western Europe from arctic to temperate latitudes. Up to three populations of each species were collected in the summer and ks measured within 48 h. Summer ks values were relatively high in the temperate species, Gammarus locusta, from Portugal (48°N) and Wales (53°N) and were maintained across latitudes by the conservation of translational efficiency. In sharp contrast, summer ks remained remarkably low in the boreal/temperate species G. duebeni from Wales, Scotland (58°N) and Tromsø (70°N), probably as a temporary energy saving strategy to ensure survival in rapidly fluctuating environments of the high intertidal. Values for ks increased in acclimated G. duebeni from Scotland and Tromsø showing a lack of compensation with latitude. In the subarctic/boreal species, G. oceanicus, summer ks remained unchanged in Scotland and Tromsø but fell significantly in Svalbard (79°N) at 5°C, despite a slight increase in RNA content. At 79°N, mean ks was 4.5 times higher in the circumpolar species G. setosus than in G. oceanicus due to a doubling in RNA content. The relationship between whole-animal protein synthesis rates and natural thermal gradients is complex, varies between species and appears to be associated with local temperatures and their variability, as well as changes in other environmental factors.

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“…All amplifications were repeated at least twice to check the stability of amplification products. Bands were scored as present (1) or absent (0) (Tansley & Brown, 2000; Costa et al , 2004a, b) by eye and only unequivocal bands were scored, with weak bands not being included. To ensure data accuracy, all samples were scored twice by the same individual, and the second round of scoring was conducted without reference to data from the initial round (Star et al , 2003).…”

Section: Methodsmentioning

confidence: 99%

Exploring molecular variation in the cosmopolitan Caprella penantis (Crustacea: Amphipoda): results from RAPD analysis

et al. 2009

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Eight populations of Caprella penantis, three of Caprella dilatata and two of Caprella andreae, collected from different sites all over the world, were selected for genetic study. Thirteen primers were tested, and the phenogram, based on the similarity coefficient of Nei & Li and the UPGMA method, separated clearly C. dilatata and C. andreae from the populations of C. penantis, supporting the validity of these three species, traditionally considered altogether under the old ‘acutifrons’ complex. Populations of C. penantis (including, at least, forms simulatrix, testudo and lusitanica) from Spain, Portugal, Morocco, Japan and Brazil were clustered together in the RAPD analysis, indicating that, probably, all the specimens of C. penantis could belong to the same species, in spite of morphological variations in the pleura, gills, robustness and presence/absence of proximal projection in adult male gnathopod 2 propodus. The only population which showed genetic differentiation within the C. penantis complex was the form gibbosa from Coquimbo, Chile. Future analysis based on different molecular approaches (mtDNA, 18S rRNA, ISSR) and additional material from other world areas, should be conducted to confirm these results.

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RAPD analysis of southern populations of Gammarus locusta: comparison with allozyme data and ecological inferences

Cited by 15 publications

References 51 publications

Invasion or invisibility: using genetic and distributional data to investigate the alien or indigenous status of the Atlantic populations of the peracarid isopod, Stenosoma nadejda (Rezig 1989)

Invasion or invisibility: using genetic and distributional data to investigate the alien or indigenous status of the Atlantic populations of the peracarid isopod, Stenosoma nadejda (Rezig 1989)

Influence of Natural Thermal Gradients on Whole Animal Rates of Protein Synthesis in Marine Gammarid Amphipods

Exploring molecular variation in the cosmopolitan Caprella penantis (Crustacea: Amphipoda): results from RAPD analysis

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