RAPD analysis of the relationship of North and South American subspecies of <i>Fragaria chiloensis</i>

Porebski, Sue; Catling, Paul M.

doi:10.1139/b98-180

Cited by 4 publications

(3 citation statements)

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“…Our sampling error was comparable to other studies of ployploid species with RAPD data sets of ≈100 bands. (Aruna et al, 1993;Levi and Rowland, 1997;Porebski and Catling, 1998).…”

Section: Resultsmentioning

confidence: 99%

Genetic Relationships among Lowbush Blueberry Genotypes as Determined by Randomly Amplified Polymorphic DNA Analysis

Burgher¹,

Jamieson²,

Lu³

2002

jashs

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Twenty-six genotypes of lowbush blueberry (Vaccinium angustifolium Aiton) representing four geographical zones (Maine, United States; New Brunswick, northern Nova Scotia, and western Nova Scotia, Canada) were selected to obtain DNA fingerprints and to estimate genetic similarity by randomly amplified polymorphic DNA analysis. The genotypes were either native accessions or selections from crosses involving native accessions as parents or grandparents. Thirty 10-base RAPD primers were initially screened; 11 proved to be polymorphic, resulting in 73 consistent RAPD bands. All 26 genotypes could be distinguished by their unique RAPD banding patterns and three unlabeled samples were correctly identified. The RAPD band data set was analyzed with Genstat5 to calculate similarity and distance matrices. Average similarity across all genotypes was 56%. Results from average linkage cluster analysis were used to construct a dendogram which demonstrated six main clusters with an average similarity linkage of 70%. The selection `Fundy' and its parent `Augusta' clustered at 77% similarity. The corresponding principal coordinate analysis supported the clusters and identified two distinct outliers. There was a small association by geographic grouping for five genotypes from Maine. It was concluded that RAPD analysis is a useful tool for genotypic identification and estimates of genetic similarity in lowbush blueberry.

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“…Our sampling error was comparable to other studies of ployploid species with RAPD data sets of ≈100 bands. (Aruna et al, 1993;Levi and Rowland, 1997;Porebski and Catling, 1998).…”

Section: Resultsmentioning

confidence: 99%

Genetic Relationships among Lowbush Blueberry Genotypes as Determined by Randomly Amplified Polymorphic DNA Analysis

Burgher¹,

Jamieson²,

Lu³

2002

jashs

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“…The earliest studies of taxonomy and diversity relationships in strawberry were conducted mainly with RAPD markers; though they are poorly reproducible between labs, they have been used to draw general conclusions regarding the structure of wild populations. Porebski and Catling [57] used 12 RAPD primers to determine that North American subspecies F. chiolensis subsp. pacifica and subsp.…”

Section: Genetic Diversity and Population Structurementioning

confidence: 99%

Applications of molecular markers in strawberry

Whitaker

2011

Journal of Berry Research

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This review examines current and potential applications of molecular markers in strawberry (Fragaria spp.). The most prevalent uses of markers in strawberry are for analyses of genetic diversity, genetic mapping, and cultivar identification. Cultivar identification is by far the most important use of markers to date for the protection of intellectual property as well as to confirm the identity of nursery stock through multiple cycles of asexual propagation. These applications have been accelerated by continuous discovery of simple sequence repeat markers and the development of multiplexed sets. Mapping studies have confirmed the allopolyploid genome structure of cultivated strawberry and shed light onto genetic events that punctuate its domestication. Though marker-trait associations have been identified for disease resistance, photoperiodic flowering, and sex expression, markerassisted selection in breeding is currently confined to a few entities in the private sector. Pedigree-based analysis holds promise for identifying additional marker-trait associations and simultaneously mining alleles in multiple genetic backgrounds. Meanwhile, the completion of the diploid strawberry genome sequence promises to accelerate candidate-gene approaches for marker discovery.

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“…Contrary to general opinion (Bringhurst and Khan, 1963;Senanayake and Bringhurst, 1967), the diploid species F. vesca did not show levels of affinity with the cultivated octoploid strawberry that would necessarily suggest it to be ancestral. Porebski and Catling (1998) used 12 RAPD primers to investigate intraspecific relationships between five North American F. chiloensis ssp. lucida accessions, 15 North American ssp.…”

Section: Genetic Fingerprinting Gene Tagging and Mappingmentioning

confidence: 99%

SNiPs, Chips, BACs, and YACs: Are Small Fruits Part of the Party Mix?

Hokanson¹

2001

HortSci

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V. corymbosum L. selections and hybrids; 4) southern highbush blueberries, which are basically the same as the more common highbush types, with the addition of genes from the low-chilling species V. darrowii Camp, as well as from V. angustifolium Ait., V. ashei Reade, and V. tenellum Ait. (Ballington et al., 1991a; Lyrene, 1990a); and 5) rabbiteye blueberries, which are all wild selections or hybrids derived from V. ashei. In general, current breeding objectives for the numerous blueberry breeding programs include: 1) expansion of the range of production through broader soil adaptation (upland mineral soils); 2) adaptation to lower chill regions or colder production regions through increased bud tolerance or delayed flowering combined with earlier fruit maturation; and 3) increased disease resistance. Genetic fingerprinting, gene tagging, and mapping Rabbiteye blueberry cultivars constitute a very narrow germplasm base, which has been recognized as a problem (Lyrene, 1981b). To determine the magnitude of the problem and devise a method to widen the germplasm base, Aruna et al. (1993) utilized 20 randomly amplified, polymorphic DNA (RAPD) primers to determine genetic relatedness among four native clones and 15 improved cultivars of rabbiteye blueberry. In an extension of the previous study, a cultivar key for economically important rabbiteye blueberry was created using 11 DNA fragments amplified by four RAPD primers (Aruna et al., 1995). Clustering of genotypes generally made sense in light of known pedigree information. Overall, there was a trend toward lower genetic distances within advanced generations from the wild selections. Levi and Rowland (1997) utilized 15 RAPD markers and three simple-sequence repeat (SSR)-anchored primers to differentiate 15 highbush blueberry and two rabbiteye cultivars and one southern lowbush selection from the wild. However, the molecular data showed clustering within V. corymbosum that did not agree with pedigree information. In a similar study, 26 wild lowbush blueberry clones (V. angustifolium), including six named cultivars and 12 selections, were screened with a total of 30 RAPD primers (Burgher et al., 1998). All 26 clones could be unambiguously identified with 11 of the primers, and clusters matched known relationships among the clones fairly well, showing trends relating to geographic origin. Several efforts are under way to create genetic linkage maps for cultivated blueberry in order to tag quantitative trait loci (QTLs) related to chilling requirement, cold hardiness, and soil adaptation. Rowland and Levi (1994) constructed a RAPD-based genetic linkage map of diploid blueberry based on a cross between an F 1 hybrid created between V. darrowi x V. elliottii Chapm. and another V. darrowi genotype. The map was constructed from over 70 markers and consisted of 12 linkage groups, which represented the basic chromosome number for blueberry. More recently, Rowland et al. (1999) developed RAPD-based genetic linkage maps based on crosses between F 1 hybrids of V. darrowi and V. c...

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RAPD analysis of the relationship of North and South American subspecies of Fragaria chiloensis

Cited by 4 publications

References 0 publications

Genetic Relationships among Lowbush Blueberry Genotypes as Determined by Randomly Amplified Polymorphic DNA Analysis

Genetic Relationships among Lowbush Blueberry Genotypes as Determined by Randomly Amplified Polymorphic DNA Analysis

Applications of molecular markers in strawberry

SNiPs, Chips, BACs, and YACs: Are Small Fruits Part of the Party Mix?

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