2001
DOI: 10.1111/j.1348-0421.2001.tb01280.x
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RAPD‐ and actA Gene‐Typing of Listeria monocytogenes Isolates of Human Listeriosis, the Intestinal Contents of Cows and Beef

Abstract: Seventy-five L monocytogenes isolates of human listeriosis, the intestinal contents of cows and beef were divided into 5 major clusters, 17 sub-clusters and 28 minor clusters by typing using random amplification of polymorphic DNA (RAPD). According to their major RAPD category, L monocytogenes isolates serotyped as 1I2b and 4b were distinguished from L monocytogenes isolates of serovars 1I2a and 1I2c. Moreover serovar 4b was distinguished from serovar 1/2b by a difference in the RAPD sub-cluster category. All … Show more

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Cited by 11 publications
(7 citation statements)
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“…So far, several DNA fragment-size-based methods, such as PFGE, REP-PCR, and ERIC-PCR, have been applied to L. monocytogenes subtyping (Jersek et al 1999;Inoue et al 2001;Fonnesbech-Vogel et al 2004;Wojciech et al 2004;Chou and Wang 2006). To our knowledge, no PCR-based subdivision method was compared to typing obtained by sequence analysis.…”
Section: Discussionmentioning
confidence: 96%
“…So far, several DNA fragment-size-based methods, such as PFGE, REP-PCR, and ERIC-PCR, have been applied to L. monocytogenes subtyping (Jersek et al 1999;Inoue et al 2001;Fonnesbech-Vogel et al 2004;Wojciech et al 2004;Chou and Wang 2006). To our knowledge, no PCR-based subdivision method was compared to typing obtained by sequence analysis.…”
Section: Discussionmentioning
confidence: 96%
“…The applicability of the technique for typing of Listeria species and sub-typing of L. monocytogenes strains has been documented in many studies from many different laboratories [205,[236][237][238][239][240][241] and was also shown in an interlaboratory trial using three universal primers [242]. RAPD typing is an excellent tool for epidemiological studies and has been extensively used to link L. monocytogenes strains isolated from listeriosis cases to foods that were implicated in outbreaks [238,[243][244][245][246][247][248][249][250]. RAPD has been widely employed to type isolates from poultry plants [206,251], smoke houses [252][253][254][255], dairy environments [247], pork processing and slaughtering plants [158] and to either exclude or include implicated food products.…”
Section: Pcr-based Typing Methodsmentioning
confidence: 99%
“…PCR products were digested separately by using HhaI and HpaII for hly, and ApoI, HinfI, and HhaI for inlA. For actA, PCR amplification of the proline-rich region, with primers PR5 and PR3 was performed according to Inoue et al (9). Two alleles, characterized by the presence of one proline-rich unit (518-bp amplification product) or two (623-bp amplification product), were classified as actA type II and actA type I, respectively.…”
Section: Pcr-rflp Of Virulence Genesmentioning
confidence: 99%