RAPD and ISSR markers indicate high genetic variation within Lobathallia radiosa in Turkey

Abstract

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) methods were used for investigating genetic variation within L. radiosa and for delimiting species within the genus Lobothallia. Ten RAPD and 11 ISSR primers produced a total of 261 and 260 reproducible bands, respectively, and all of which were polymorphic for the two markers, suggesting high genetic variability within L. radiosa. Genetic distances between the samples of L. radiosa for the RAPD and ISSR analyses ranged from 51 to 93% and from 56 to 92%, respectively. The average was 71.2% for the RAPD markers while it was 80.4% for the ISSR markers. Although a high number of sample-specific bands (31 for RAPD and 16 for ISSR) were seen in L. radiosa, no species-specific band was observed. The RAPD-based dendrogram clustered the samples of L. alphoplaca into one group except for the samples from Kayseri and Nigde, whereas it divided the samples of L. radiosa into two main groups. On the other hand, the ISSR-based dendrogram resulted in four main groups. While the first main group included the three samples of L. alphoplaca, the other three main groups consisted of the samples of L. radiosa. Both RAPD-and ISSR-based dendrograms partially grouped the samples of L. radiosa and L. alphoplaca based on their geographical origins.

Genetic diversity of natural psammophilous populations of Hypogymnia physodes (L.) Nyl. on Polish seacoast dunes

“…Such a high genetic diversity between local populations of lichens has been already described [2,13]. Moreover, another studies have shown even higher intraspecific genetic diversity than noted in our research, e.g., in the lichen species Lobothallia alphoplacaand, L. radiosa [14] or in the fungus Pisolithus tinctorius [15]. The reason of such high genetic may be the habitat whose isolation reduces the flow of genes between populations [16][17][18].…”

“…The reason of such high genetic may be the habitat whose isolation reduces the flow of genes between populations [16][17][18]. The high variation is an important trait because it plays a role under environmental changes, determining the survival of organisms [14,19,20]. The evaluation of two analyzed groups of H. physodes, the first growing on the bark of pine trees and the second on the sand, confirms the hypothesis that lichens are sensitive to substrate features [21].…”

RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

“…As a result of the amplifications with the use of the examined Viburnum genotypes, 1160 (RAPD) and 873 (ISSR) amplicons were scored as the effect of amplification of 690 RAPD and 418 ISSR loci, respectively. The length of the amplified products corresponded to the number of products amplified, the number of primers used, and the length of products mostly generated with these methods and presented in papers by Sharma et al (2009), Williams et al (1990), Yüzbaşioğlu et al (2011) and Zietkiewicz et al (1994). We demonstrated high genetic variability among the examined Viburnum objects.…”

“…Genotype-specific ISSR products generated for 13 genotypes of Viburnum reasons, such as delimitation of species, conservation of endangered species, analysis of the population' structure of a species and construction of phylogenetic relationships among species in fungi, plants and animals (Yüzbaşioğlu et al, 2011). The invention of PCR led to the development of fast and inexpensive molecular markers, such as RAPD and ISSR (Arseniuk 2000; Czembor and Sharma et al, 2009;Gajera et al, 2011;Godwin et al, 1997;Williams et al, 1990;Yüzbaşioğlu et al, 2011). Because of the longer primers used, ISSR markers reported to be more reproducible than RAPD markers (Godwin et al, 1997).…”

“…The first one amplifies DNA using 10-mer primers, while the second one involves amplification of DNA sequence between two adjacent and inversely oriented microsatellites usually by using 18-24-mer primers (Williams et al, 1990;Ziętkiewicz et al, 1994). RAPD and ISSR are popular DNA fingerprinting methods because they are quick, inexpensive, highly polymorphic and also they do not require any sequence information about the under study genome (Gajera et al, 2011;Yüzbaşioğlu et al, 2011).…”