RAPD and RFLP mapping of the bacterial blight resistance gene xa-13 in rice

Abstract

Bacterial blight (BB) caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the most serious diseases of rice. The recessive gene xa-13 confers resistance to Philippine race 6 of Xoo. To tag xa-13 with molecular markers, RAPD analysis was conducted with the combined use of near-isogenic lines and bulked segregant analysis. From the survey of 260 arbitrary 10-nucleotide primers, one primer (OPAC05) was detected to amplify specifically a 0.9-kb band from the DNA of susceptible plants. The distance between the RAPD marker OPAC05-900 and xa-13 was estimated to be 5.3 cM. The RAPD marker was then mapped on chromosome 8 using a mapping population of doubled haploid lines derived from the cross of IR64/Azucena. The linkage between RFLP markers and the RAPD marker was analyzed using an F2 population of 135 plants derived from a cross between a near-isogenic line for xa-13, IR66699-5-5-4-2, and IR24. No recombinants were found between RZ28 and CDO116 and their distance from xa-13 was estimated to be 4.8 cM. RG136 was located at 3.7 cM on the other side of xa-13. The mapping of xa-13 with closely linked DNA markers provides the basis for marker-aided selection for rice improvement.

Detection of bacterial blight resistance genes in basmati rice landraces

“…The recessive genes xa5 and xa13 prevented BB epidemics by conferring resistance to a wide spectrum of X. oryzae races and have been mapped on chromosomes 5 and 8, respectively (Yoshimura et al, 1995;Zhang et al, 1996). MAS is a pivotal tool for the pyramiding of such recessive genes in the presence of dominant genes such as Xa4 and Xa7.…”

“…These limitations can be addressed by marker-assisted selection (MAS), which enables the evaluation of the expression status of resistance gene(s) and allows for pyramiding of multiple resistance genes in a desirable genetic background. Polymerase chain reaction (PCR)-based DNA markers for some of these genes have been identified: MP1 and MP2 for Xa4 (Ma et al, 1999), RM122 for xa5 (Chen et al, 1997), M5 for Xa7 (Porter et al, 2003), and RG136 for xa13 (Zhang et al, 1996). These markers have been employed to identify germplasm containing these genes (Blair and McCouch, 1997) and develop rice cultivars with single and multiple resistance genes (Perumalsamy et al, 2010;Rajpurohit et al, 2010).…”

Association of quantitative trait loci for plant height with major dwarfing genes in rice

“…These maps have been used to locate a series of major genes governing resistance to bacterial blight (Ronald et a!., 1992;Yoshimura et al, 1992;Zhang et al, 1994), blast (Yu, 1991), brown plant hopper (Ishii et a!., 1994) and gall midge (Williams et a!., 1994) and genes for morphological characters such as plant height (Yu, 1991;Ideta et a!., 1992;Kishimoto et at., 1993;Liang et at., 1994).…”

Applications of molecular markers for bacterial blight resistant varieties in rice (Oryza sativa L.)

“…Because of Quantitative 'Complementation' (Basavaraj et al, 2010) or 'synergistic action' (Zhang et al, 1996) pyramided variety gives longer durability. The chance of becoming virulence to two or three genes is much less compared to single gene (Mundt et al, 1990).…”