RAPD and SCAR markers linked to the Ma1 root-knot nematode resistance gene in Myrobalan plum (Prunus cerasifera Ehr.)

Lecouls, A.C.; Rubio-Cabetas, M. J.; Minot, J. C.; Voisin, R; Bonnet, A.; Salesses, G.; Dirlewanger, Elisabeth; Esmenjaud, Daniel

doi:10.1007/s001220051240

Cited by 38 publications

(27 citation statements)

References 18 publications

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“…However Myrobalan has been described as a total or partial self-incompatible species (Layne & Sherman, 1986;Esmenjaud et al, 1996;Lecouls et al, 1999); this is in agreement with the low fruit set rate obtained in self-pollinated flowers. In emasculated but non-pollinated flowers accidental pollinations also occurred since an average of 1.1% of the flowers set fruit, a value close to that obtained in self-pollinated flowers (0.9%) indicating that a similar accidental process could happen.…”

Section: Discussionsupporting

confidence: 62%

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

et al. 2006

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SummaryThe incidence of fortuitous pollination on interspecific hybridizations of the plum rootstock Myrobalan with the apricot cultivars 'Moniquí' and 'Moniquí Borde' was assessed in this work. Progeny was originated through hand pollination of emasculated flowers of three Myrobalan clones, without bagging, in 1998 and 1999. Fruit set was low and variable among years (1.8-8.0%), but higher than the level of accidental pollination measured with emasculated and non-pollinated flowers (1.2%). Molecular characterization of the progeny was performed with three SSR markers showing that only 28% of the seedlings, obtained by in vitro germination and culture of immature embryos, were hybrids. This represents a lower percentage than expected, and is explained here by the low viability of hybrid embryos and seedlings. The use of molecular markers is discussed in terms of a convenient method for an early identification of putative hybrids in breeding programs with low setting crosses, where the proportion of non-hybrids is magnified.

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Section: Discussionsupporting

confidence: 62%

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

et al. 2006

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“…Markers tightly linked to a resistance gene (Ma͞ma) from Myrobalan plum have been identified (42). This gene, located on G7, and another one from peach cv.…”

Section: Rosaceae Macrosynteny Compared To That Of Other Plant Familiesmentioning

confidence: 99%

Comparative mapping and marker-assisted selection in Rosaceae fruit crops

Dirlewanger¹,

Graziano²,

Joobeur³

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

476

456

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The development of saturated linkage maps using transferable markers, restriction fragment length polymorphisms, and microsatellites has provided a foundation for fruit tree genetics and breeding. A Prunus reference map with 562 such markers is available, and a further set of 13 maps constructed with a subset of these markers has allowed genome comparison among seven Prunus diploid (x ‫؍‬ 8) species (almond, peach, apricot, cherry, Prunus ferganensis, Prunus davidiana, and Prunus cerasifera); marker colinearity was the rule with all of them. Preliminary results of the comparison between apple and Prunus maps suggest a high level of synteny between these two genera. Conserved genomic regions have also been detected between Prunus and Arabidopsis. By using the data from different linkage maps anchored with the reference Prunus map, it has been possible to establish, in a general map, the position of 28 major genes affecting agronomic characters found in different species. Markers tightly linked to the major genes responsible for the expression of important traits (disease͞ pest resistances, fruit͞nut quality, self-incompatibility, etc.) have been developed in apple and Prunus and are currently in use for marker-assisted selection in breeding programs. Quantitative character dissection using linkage maps and candidate gene approaches has already started. Genomic tools such as the Prunus physical map, large EST collections in both Prunus and Malus, and the establishment of the map position of high numbers of ESTs are required for a better understanding of the Rosaceae genome and to foster additional research and applications on fruit tree genetics. T he major temperate fruit tree crops, apple (Malus ϫ domestica), peach (Prunus persica), cherry (Prunus avium and Prunus cerasus), plum (Prunus domestica and Prunus salicina), apricot (Prunus armeniaca), almond (Prunus dulcis), pear (Pyrus communis), quince (Cydonia oblonga), and loquat (Eriobotrya japonica), belong to the Rosaceae family. This also includes some other important crops such as strawberry (Fragaria ϫ ananassa) and rose (Rosa spp.). Most of these species are woody perennials with a long intergeneration period due to their juvenile phase and large plant sizes, which make them poorly suited organisms for classical genetic analysis. On the other hand, fruit trees have some advantageous features such as a long life, the existence of efficient methods of vegetative reproduction, the possibility of making interspecific crosses (frequent at the congeneric level), and a small basic genome; e.g., wild strawberry (Fragaria vesca) has a haploid genome size of 164 Mbp (1), and peach has a haploid genome size of 290 Mbp (2). Until recently, only very limited information existed on the genetics of phenotypic characters of simple inheritance; only 31 major genes had been described in peach (3), the best characterized of the Prunus species, or three genes in almond (4).The breeding methods used in these species have undergone very few changes over the last 50 years, and th...

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“…The labeled PCR products were separated on a 5% denaturing polyacrylamide gel containing 7.5 M urea, in 0.5 TBE running buffer then dried and autoradiographed on X-ray films. For the SCAR or STS (sequence tagged site) markers, PCR amplifications were performed as described by Lecouls et al (1999) for SCAL19 and SCAN12, Lu et al (1999) for EAA/MCAT10 STS, and Yamamoto and Hayashi (2002) for OPAP4, OPS14a and OPA11. To recover polymorphism for the SCAL19 marker in the resistant Japanese plum J.222, the multiplex amplification procedure reported by Lecouls (2000) using two forward and one reverse primer was performed.…”

Section: Materials Segregating For Amygdalus Rkn Genesmentioning

confidence: 99%

“…Two reliable SCAR (Sequence Characterized Amplified Region) markers, SCAL19 690 and SCAFLP2 202 , were shown to be linked in coupling to the dominant resistance alleles Ma1 and Ma3 (Lecouls et al 1999;Bergougnoux et al 2002). They have been identified by bulked segregant analysis (BSA) (Michelmore et al 1991) using intraspecific progenies involving P.2175 (Ma1 ma) and several susceptible parents (ma ma).…”

Section: Introductionmentioning

confidence: 98%

Location of independent root-knot nematode resistance genes in plum and peach

Claverie¹,

Bosselut²,

Lecouls³

et al. 2003

Theor Appl Genet

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Prunus species express different ranges and levels of resistance to the root-knot nematodes (RKN) Meloidogyne spp. In Myrobalan plum ( Prunus cerasifera), the dominant Ma gene confers a high-level and wide-spectrum resistance to the predominant RKN, Meloidogyne arenaria, Meloidogyne incognita, Meloidogyne javanica and the isolate Meloidogyne sp. Florida which overcomes the resistance of the Amygdalus sources. In Japanese plum ( Prunus salicina), a similar wide-spectrum dominant resistance gene, termed R(jap), has been hypothesized from an intraspecific segregating cross. In peach, two crosses segregating for resistance to both M. incognita and M. arenaria were used to identify single genes that each control both RKN species in the Shalil ( R(Mia557)) and Nemared ( R(MiaNem)) sources. Localisation of these genes was made possible using the RFLP and SSR- saturated reference Prunus map TxE, combined with a BSA approach applied to some of the genes. The Ma1 allele carried by the Myrobalan plum accession P.2175 was localised on the linkage group 7 at an approximate distance of 2 cM from the SSR marker pchgms6. In the Japanese plum accession J.222, the gene R(jap) was mapped at the same position in co-segregation with the SSR markers pchgms6 and CPPCT022. The peach genes R(Mia557) and R(MiaNem), carried by two a priori unrelated resistance sources, were co-localized in a subtelomeric position on linkage group 2. This location was different from the more centromeric position previously proposed by Lu et al. (1999) for the resistance gene Mij to M. incognita and M. javanica in Nemared, near the SSR pchgms1 and the STS EAA/MCAT10. By contrast, R(Mia557) and R(MiaNem) were flanked by STS markers obtained by Yamamoto and Hayashi (2002) for the resistance gene Mia to M. incognita in the Japanese peach source Juseitou. Concordant results for the three independent sources, Shalil, Nemared and Juseitou, suggest that these peach RKN sources share at least one major gene resistance to M. incognita located in this subtelomeric position. We showed that plum and peach genes are independent and, thus, can be pyramided into interspecific hybrid rootstocks based on the plum and peach species.

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RAPD and SCAR markers linked to the Ma1 root-knot nematode resistance gene in Myrobalan plum (Prunus cerasifera Ehr.)

Cited by 38 publications

References 18 publications

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

Comparative mapping and marker-assisted selection in Rosaceae fruit crops

Location of independent root-knot nematode resistance genes in plum and peach

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