2010
DOI: 10.4238/vol9-2gmr783
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RAPD assay of wild-type olives in Turkey

Abstract: ABSTRACT. Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20, OP-I 1-20, OP-Q 1-20, and OP-J 1-5). Thirtytwo of these primers yielded 115 highly polymorphic bands. The m… Show more

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Cited by 11 publications
(15 citation statements)
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“…It is estimated that 900 million olive trees cover over 10 million hectares worldwide (Sesli and Yeğenoğlu 2009). Dermechea et al (2013) stated that Mediterranean countries produce approximately 2.5 million metric tons/year olive oil.…”
Section: Introductionmentioning
confidence: 99%
“…It is estimated that 900 million olive trees cover over 10 million hectares worldwide (Sesli and Yeğenoğlu 2009). Dermechea et al (2013) stated that Mediterranean countries produce approximately 2.5 million metric tons/year olive oil.…”
Section: Introductionmentioning
confidence: 99%
“…In order to solve this problem, variations within and between populations of taxa were pointed out by using some statistical analyses such as Statistica version 8.0 for principal component analysis (PCA) and cluster analysis (CA) using an unweighted pair group method (UPGMA) analysis and Popgen 32. According to the results of statistical analyses, it was attempted to draw the most possible borders of taxa based on the DNA bands obtained from RAPD analyses (Sesli and Yegenoglu, 2009;Açık et al, 2009;Kavalcıoglu et al, 2010) and a better phenetic classification by using molecular characters showing high correlations with each other. Figure 1).…”
Section: Introductionmentioning
confidence: 99%
“…Although more than 2600 distinct olive cultivars have been described, this may be an underestimation, given of the lack of data on minor local cultivars in many olive-growing areas. This broad genetic diversity has been characterized in different eastern and western Mediterranean countries based both on morphological and on molecular characters (Cipriani et al, 2002;Owen et al, 2005;Rallo et al, 2005;Sarri et al, 2006;Cordeiro et al, 2008;Ipeck et al, 2009;Fendri et al, 2010;Roubos et al, 2010;Sesli and Yeğenoğlu, 2010). Molecular methods for olive cultivar fingerprinting have been demonstrated to be effective, but microsatellite or simple sequence repeat (SSR) analysis is becoming the preferred choice for its high discriminatory power and simpler interpretation (Bracci et al, 2011).…”
Section: Introductionmentioning
confidence: 99%