RAPD assisted selection of black gram (Vigna mungo L. Hepper) towards the development of multiple disease resistant germplasm

Abstract

Black gram (Vigna mungo L. Hepper), is an extensively studied food crop which is affected by many abiotic and biotic factors, especially diseases. The yield potential of Black gram is shallow due to lack of genetic variability and biotic stress susceptibility. Core biotic stress factors include mung bean yellow mosaic virus (MYMV), urdbean leaf crinkle virus (UCLV), wilt (Fusarium oxysporum) and powdery mildew (Erysiphe polygoni DC). Although many studies determine resistant varieties to a particular disease, however, it is often complimented by low yield and susceptibility to other diseases. Hence, this study focuses on investigating the genetic relationships among three varieties and nine accessions of black gram having disease resistance to previously described diseases and susceptibility using random amplified polymorphic deoxyribonucleic acid (RAPD) markers. A total of 33 RAPD primers were used for diversity analysis and yielded 206 fragments. Number of amplified fragments ranged from two (OPN-1) to 13 (OPF-1). The highest similarity coefficient was observed between IC-145202 and IC-164118 (0.921), while lowest similarity was between PU-31 and IC-145202 (0.572). The genetic diversity obtained in this study along with disease analysis suggests PU31as a useful variety for the development of markers linked to MYMV, UCLV, wilt and powdery mildew resistance by marker-assisted back cross breeding and facilitates the production of crosses with multiple disease resistance.

Assessment of Genetic Diversity in Promising Sugarcane Mutants Developed Through Chemical Mutagenesis

“…Ali et al [15] reported RAPD markers for characterization of rust resistance in sugarcane. Vishalakshi et al [16] showed RAPD assisted Black gram germplasm selection for multiple disease resistance. Nair et al [10,17] and Ardiel et al [17] have shown the utility of RAPD analysis in identifying DNA sequence variations with resistance and susceptibility by converting them into SCAR markers.…”

Genetic diversity analysis of advanced breeding lines derived from interspecific and intervarietal crosses of black gram based on morphological and molecular markers

“…Such comparative studies in legumes involving RAPD and ISSR markers have been successfully carried out previously in crops like Black gram (Souframanien and Gopalakrishna 2004), Vigna species (Dikshit et al 2007) and Rice (Ravi et al 2003). Also, RAPD markers has been used to study the genetic interraltionships among blackgram genotypes to develop multidisease resistance germplasm (Vishalakshi et al 2017).…”

Construction of engineered RuBisCO Kluyveromyces marxianus for a dual microbial bioethanol production system

“…The thermotolerant yeast Kluyveromyces marxianus is a promising candidate for fuel ethanol production as it possesses several advantageous characteristics for biotechnological applications such as the faster growth rate relative to Kluyveromyces lactis [ 1 ] or Saccharomyces cerevisiae [ 2 ], the ability to assimilate a wide range of sugars [ 3 ], thermotolerance [ 4 – 6 ], secretion of lytic enzymes and the ability to produce ethanol at elevated temperatures [ 7 ]. Specifically, K .…”