RAPD Fingerprint to Appraise the Genetic Fidelity of In Vitro Propagated Araucaria excelsa R. Br. var. glauca Plantlets

Sarmast, Mostafa Khoshhal; Sher, Hassan; Ramezani, Alireza; Abolimoghadam, Ali Asghar; Niazi‎, Ali; Khosh-Khui, Morteza

doi:10.1007/s12033-011-9421-7

Cited by 14 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(Prakash et al, 2004), Cymbopogon flexuosus (Bhattacharya et al, 2008), papaya (Homhuan et al, 2008) and cotton (Jin et al, 2008). Also, there are several reports about TDZ affecting the DNA stability of plants propagated in vitro (Sarmast et al, 2012;Roy et al, 2012;Khattab et al, 2014). In contrast, the RAPD analysis performed in our study detected no polymorphism within plants regenerated through the same regeneration pathway, whereas the RAPD analysis with the same decamer primers revealed considerable polymorphism within iris plants derived from seeds.…”

Section: Discussioncontrasting

confidence: 68%

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Stanišić

Raspor

Ninković

et al. 2015

South African Journal of Botany

View full text Add to dashboard Cite

Efficient protocols, safe from somaclonal variation, were developed for regeneration of Iris sibirica plants via organogenesis and somatic embryogenesis from leaf-base explants cultivated on Murashige and Skoog media supplemented with thidiazuron (TDZ, 1.0 mg/l) or 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l). The morphogenic response and callus formation efficiency differed significantly between 2,4-D (80.9%) and TDZ (67%) morphogenesis induction treatments. TDZ induced only organogenic calli, while calli obtained with 2,4-D were composed of three types differing in color and consistency: white, friable -embryogenic calli (4.5%, 3.8 mg/explant), green, compact -organogenic calli (12.4%, 48.4 mg/explants) and yellow -nonregenerative calli (77.3%, 254.4 mg/explant). The cultivation of embryogenic calli on medium with 2,4-D and Kinetin resulted in further development of somatic embryos (54 embryos/g of calli) which germinated with a frequency of 62% after being transferred to a medium without plant growth regulators. Stable shoot cultures were established by transferring organogenic calli with shoot primordia to media with 0.1 mg/l α-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzylaminopurine (BA), while further cultivation on media of the same composition (TDZ or 2,4-D) resulted in the reduced growth and rhizogenesis, respectively. The TDZ induction treatment resulted in higher number of shoots per explant (7.9) than the 2,4-D treatment (4.3). After successful rooting and ex vitro acclimatization, plants were grown in the field and flowered to seed production. Flow cytometry, chromosome counting and random amplification of polymorphic DNA (RAPD) analysis indicated no evidence of genetic variation in plants regenerated via somatic embryogenesis or organogenesis. The results suggest that established protocols are safe for use in genetic transformation procedures or large-scale production of true-to-type I. sibirica plants.

show abstract

Section: Discussioncontrasting

confidence: 68%

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Stanišić

Raspor

Ninković

et al. 2015

South African Journal of Botany

View full text Add to dashboard Cite

show abstract

“…Explants grown in medium supplemented with TDZ, were morphologically better than other explants treated with plant growth regulators (PGRs) (Fig. 2), hence the explants treated with TDZ were used for further experiments [17]. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The potting medium consisted of a 1:1:1 (in volume) of loam, peat moss, and perlite and it was fertilized by Kristalon solution (1/1000, each 15 days). Four cm of apical stem segments of main stem were cut from seedlings and kept under running tap-water for 2 h. Because of high contamination rate, nano silver (NS) with concentration of 500 µg/ml under reduced pressure (300 mm Hg in 5 min) [16,17] was used to eradicate bacterial contamination. Then, the explants were treated with 70% ethanol for 3 min and 15% Clorox (containing 5.25% sodium hypochlorite) with 0.2% detergent for 10-20 min for surface sterilization, and then rinsed six times with sterilized distilled water.…”

Section: Decontamination Of Explants and Production Of Orthotropic Shmentioning

confidence: 99%

In Vitro Rooting of Araucaria Excelsa R. Br. Var. Glauca Using Agrobacterium Rhizogenes

Sarmast¹,

Salehi²,

Khosh-Khui³

2012

JCEA

Self Cite

View full text Add to dashboard Cite

The family Araucariaceae encompasses several evergreen forest tree species, which has a high ornamental value due to being a good specimen and having symmetrical branches. Conventional propagation of Araucaria excelsa R. Br. var. glauca by cutting has limited success because of topophysis and difficult-to-root characteristics, and grafting is accompanying incompatibility. The aim of this research was to evaluate the application of Agrobacterium rhizogenes as well as the IBA, NAA and ancillary compounds potential to increase the rooting of this plant under in vitro condition. Neither ancillary compounds such as salicylic acid, putrescine nor hydrogen peroxide affected the rooting of this recalcitrant species. Subculturing in vitro shoots to MS medium containing 7.5 μM of both IBA and NAA for 15 days before being moved to hormone-free half-strength MS medium, resulted in a 33% increase of rooting of shoots each with one or two roots. Using Agrobacterium rhizogenes strain K599 improved rooting percentage up to 40%. Green fluorescents protein (GFP) gene, as a reporter gene, was employed to verify the successful transformation.

show abstract

“…Regarding to PGRs, MNS/E was not always correlated to MLS/E. It is shown that higher concentration of PGRs especially cytokinins results in somaclonal variation in plants (Hartmann et al 2011;Sarmast et al 2012). The highest axillary shoots formation ability occurred on MS medium supplemented with Fig.…”

Section: Discussionmentioning

confidence: 96%

Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants

Sarmast

Salehi

Khosh-Khui

2012

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

The objectives of the present work were in vitro propagation of Araucaria excelsa R. Br. var. glauca Carrière (Norfolk Island pine) with focus on the evaluation of the mean number of shoots per explant (MNS/E) and mean length of shoots per explants (MLS/E) produced by different parts of the orthotropic stem of A. excelsa R. Br. var. glauca in response to plant growth regulators. Norfolk Island pine axillary meristems responded very well to the 2-iso-pentenyl adenine (2iP) and thidiazuron (TDZ) levels. Explants taken from stem upper segments in the media containing 2iP had a higher MNS/E (3.47) and MLS/E (6.27 mm) in comparison to those taken from stem lower segments, which were 0.71 and 0.51 mm, respectively. Using 0.045 μM TDZ in the MS medium not only resulted in 4.60 MNS/E with 7.08 mm MLS/E but proliferated shoots showed a good performance as well. Investigating the best position of stem explant on mother plant as well as the best concentrations of growth regulators were performed which were useful for efficient micropropagation of this plant. Thirty three percent of explants were rooted in the MS medium containing 3 % sucrose, supplemented with 7.5 μM of both NAA and IBA for 2 weeks before transferring to a half strength MS medium without any growth regulator. Plantlets obtained were acclimatized and transferred to the greenhouse with less than 20 % mortality. This procedure considered the first successful report for regeneration and acclimatization of A. excelsa R. Br. var. glauca plantlet through main stem explants.

show abstract

RAPD Fingerprint to Appraise the Genetic Fidelity of In Vitro Propagated Araucaria excelsa R. Br. var. glauca Plantlets

Cited by 14 publications

References 30 publications

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

In Vitro Rooting of Araucaria Excelsa R. Br. Var. Glauca Using Agrobacterium Rhizogenes

Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants

Contact Info

Product

Resources

About