RAPD fingerprinting used for discriminating among three populations of Hilsa shad (Tenualosa ilisha)

Dahle, Geir; Rahman, Muhammad Arifur; Eriksen, A.G.

doi:10.1016/s0165-7836(97)00058-1

Cited by 32 publications

(26 citation statements)

References 10 publications

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“…Dahle et al (1997) separated fish from 3 sites within 150 km of each other (Chandpur, Kuakata-Khulna region and Cox's Bazar) ( Fig. 1) with RAPD fingerprinting, implying separate populations (stocks).…”

Section: Discussionmentioning

confidence: 93%

“…Hussain et al (1998) found no evidence of different Bangladeshi stocks, including samples from these same sites. Dahle et al (1997) admitted that the difficulty in duplicating RAPD results and their uncertain heritability makes this method the least useful DNA method for detecting stock structure (Dowling et al 1996). This must cast doubt on the genetic basis of their findings, given the short distances between sites.…”

Section: Discussionmentioning

confidence: 99%

“…They have identified between 1 and 3 stocks. Dahle et al (1997) sampled at 3 sites about 150 km apart (riverine, coastal and marine) and used RADP fingerprinting to genetically separate fish from each site. Rahman et al (1997) sampled at 2 sites (riverine and coastal) using starch gel electrophoresis, and found that they were also genetically distinct.…”

Section: Introductionmentioning

confidence: 99%

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Can otolith chemistry detect the population structure of the shad hilsa Tenualosa ilisha? Comparison with the results of genetic and morphological studies

Milton¹,

Chenery²

2001

Mar. Ecol. Prog. Ser.

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The stock structure of the valuable tropical shad Tenualosa ilisha ('hilsa') has been studied in Bangladesh and India by analysing morphometric and genetic data. However, these studies had a narrow geographic scope and their results conflict. We made a comprehensive study of the stock structure of hilsa with otolith microchemistry in conjunction with complementary genetic and morphometric studies of the same fish. We examined the trace-element composition of the otolith cores of hilsa with laser-ablation inductively coupled plasma mass spectrometry. The otoliths of fish from 19 collections at 13 sites in Bangladesh and 6 collections at 4 sites from elsewhere within the species' range (Kuwait, SE India, Myanmar and Sumatra) were analysed for 8 trace elements. Samples were collected from Bangladesh mainly during 2 comprehensive surveys (1996 and 1997). When these data were analysed separately, there were significant differences in otolith composition among sites. However, when both years' data were analysed together, there were few significant differences among sites, and some sites separated by hundreds of kilometres that were sampled in different seasons and years had very similar compositions. This was in spite of both large seasonal intra-site and between-site differences in water chemistry. Repeat samples from 5 sites (4 in Bangladesh) showed that differences in otolith composition at a single site were significant and of similar magnitude to that found among sites. Our results support the conclusion from allozyme studies that there is extensive movement and mixing of hilsa throughout Bangladesh, and therefore the population should be managed as a single stock. Genetic and otolith data both showed that hilsa from SE India and Myanmar were not significantly different from fish collected in coastal areas of Bangladesh, and suggest that hilsa in the Bay of Bengal were a single stock. Both methods also separated fish from Sumatra and Kuwait from other sites, providing strong evidence of separate stocks in those regions. In contrast, morphometric studies separate fish from several nearby sites in Bangladesh, but these differences are likely to be largely due to phenotypic variability and are unlikely to be geneticallybased. Our results suggest that otolith microchemistry may be a good proxy for genetic structure at large scales where differences in water chemistry are highly likely. However, for sedentary species and those without distinct spawning and non-breeding areas, it requires both comprehensive and repeated sampling at finer scales before any confidence should be placed in the results.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Can otolith chemistry detect the population structure of the shad hilsa Tenualosa ilisha? Comparison with the results of genetic and morphological studies

Milton¹,

Chenery²

2001

Mar. Ecol. Prog. Ser.

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“…This technique has been used to estimate genetic diversity in penaeid shrimp, fish, and shellfish (Bardakci and Skibinski 1994;Garcia et al 1994;Garcia and Benzie 1995;Naish et al 1995;Dahle et al 1997;Tassanakajon et al 1997Tassanakajon et al , 1998Shi et al 1999;Song et al 1999;Zhuang et al 2001;Lakra et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity in Metapenaeus dobsoni using RAPD Analysis

et al. 2009

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“…RAPD has been used in studies of a wide range of species, such as mice (Welsh & al. 1991), fish (Bardakai & Skibinski 1994;Dahle & al. 1997) and molluscs (Crossland & al.…”

Section: Introductionmentioning

confidence: 99%

Discrimination among species of the genus Sebastes in the North Atlantic by random amplified polymorphic DNA

Johansen¹,

Dahle

2004

Sarsia

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Johansen T, Dahle G. 2004. Discrimination among species of the genus Sebastes in the North Atlantic by random amplified polymorphic DNA. Sarsia 89:478-483. SARSIAGenetic variation among and within species of the genus Sebastes [S. viviparus, S. fasciatus, S. mentella (including deep-sea and oceanic types) and S. marinus (both ordinary and giant types)] from the North Atlantic was investigated by polymerase chain reaction (PCR)-based random amplification of polymorphic DNA (RAPD). Initial analyses were based on 80 different 10 base pair primers applied to a small number of individuals of each of the four species. Four of the most variable primers were selected for analysis of the total samples from Norway, Iceland and Canada. Only one primer, OPA20, could be used to diagnostically distinguish among species. Statistical analysis clustered all samples in relation to known species structure. No genetic distance was found between ordinary and giant-type S. marinus and minor differences were observed between S. viviparus from Iceland and Norway. Sebastes mentella from the different locations clustered together, but some structuring was shown within this species. Twelve untyped Sebastes and S. fasciatus from Gulf of St. Lawrence clustered closer to S. viviparus than to S. marinus and S. mentella.

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RAPD fingerprinting used for discriminating among three populations of Hilsa shad (Tenualosa ilisha)

Cited by 32 publications

References 10 publications

Can otolith chemistry detect the population structure of the shad hilsa Tenualosa ilisha? Comparison with the results of genetic and morphological studies

Can otolith chemistry detect the population structure of the shad hilsa Tenualosa ilisha? Comparison with the results of genetic and morphological studies

Genetic Diversity in Metapenaeus dobsoni using RAPD Analysis

Discrimination among species of the genus Sebastes in the North Atlantic by random amplified polymorphic DNA

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