RAPD Marker Linked to a Gene Conferring Resistance to Race IB-9 of Pyricularia grisea in a Somaclone of the Rice Cultivar Araguaia

Araújo, Leila Garcês de; Prabhu, A. S.; Pereira, P. A. A.

doi:10.1023/b:ticu.0000022558.20925.3f

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…Diversos estudos identifi caram marcadores RAPD ligados a genes de resistência a patógenos em várias culturas, entre eles: resistência ao nematóide-de-cistoda-soja, à mancha olho-de-rã, ao míldio e ao cancroda-haste em soja (Heer et al, 1998;Carvalho et al, 2002;Chowdhury et al, 2002;Martins Filho et al, 2002;Gavioli et al, 2007); resistência à manchaangular em feijoeiro (Ferreira et al, 1999); resistência à brusone em arroz (Araújo et al, 2004 Garcia et al (2008).…”

Section: Introductionunclassified

Marcadores RAPD para detecção de resistência à ferrugem-asiática-da-soja

Costa

Unêda-Trevisoli

Pinheiro

et al. 2008

Pesq. agropec. bras.

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Resumo -Os objetivos deste trabalho foram confi rmar a herança da resistência da PI 459025 (Rpp4) à ferrugemasiática-da-soja e identifi car marcadores moleculares do tipo RAPD, ligados a este gene de resistência, em populações de soja. Pelo cruzamento dos genitores contrastantes PI 459025 x Coodetec 208 obteve-se uma população, cujas populações das gerações F 2 e F 2:3 foram artifi cialmente infectadas e avaliadas quanto à reação ao fungo Phakopsora pachyrhizi, pelo tipo de lesão (RB -resistente e TAN -suscetível). Com os resultados da avaliação fenotípica, dois "bulks" foram obtidos com DNA de plantas homozigóticas resistentes e suscetíveis, respectivamente, pela análise de "bulks" segregantes. De 600 iniciadores RAPD aleatórios, foram identifi cados três com fragmentos polimórfi cos entre os "bulks" e parentais contrastantes quanto à resistência. Pela análise do qui-quadrado, confi rmaram-se: a herança monogênica, com dominância completa quanto à resistência ao patógeno, e a segregação 3:1 para a presença de banda dos três marcadores. Os três marcadores são ligados respectivamente a 5,1, 6,3 e 14,7 cM de distância do loco de resistência, em fase de repulsão no grupo de ligação G, o que foi confi rmado pela utilização do marcador microssatélite Satt288. Estes marcadores são promissores na seleção assistida para resistência à ferrugem-asiática-da-soja.Termos para indexação: Glycine max, Phakopsora pachyrhizi, marcadores moleculares, seleção assistida. RAPD markers for detection soybean rust resistanceAbstract -The objectives of this work were to confi rm the PI 459025 inheritance of resistance (Rpp4) to Asian soybean rust pathogen and to detect RAPD markers linked to this resistance gene in soybean populations. Through the cross of the distint parental lines PI 459025 x Coodetec 208, a population was obtained, whose F 2 and F 2:3 generations had their populations artifi cially infected and evaluated for the reaction to Phakopsora pachyrhizi, by lesion type classifi cation (RB -resistant and TAN -susceptible). Using the phenotypic results, the bulked segregant analysis was performed and two DNA bulks were obtained from homozygous resistant and homozygous susceptible plants, respectively. Out of the 600 random RAPD primers analyzed, three were identifi ed as polymorphic fragments related to resistance, between contrasting bulks and parents. Through chi-square test, confi rmations were obtained for the monogenic inheritance, with complete dominance segregation for resistance to the pathogen, and the 3:1 segregation of band presence for the markers. The three markers are linked to the resistance locus, in repulsion phase, at 5.1, 6.3 and 14.7 cM from it, in the linkage group G, which was confi rmed by using the microsatellite marker Satt288. These makers are promising in assisted selection for Asian soybean rust resistance.Index terms: Glycine max, Phakopsora pachyrhizi, molecular markers, assisted selection. IntroduçãoA expansão do cultivo da soja, observada desde sua introdução comercial no Brasil na década de 70,...

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Section: Introductionunclassified

Marcadores RAPD para detecção de resistência à ferrugem-asiática-da-soja

Costa

Unêda-Trevisoli

Pinheiro

et al. 2008

Pesq. agropec. bras.

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“…The availability of different molecular markers allows characterization of genes of interest. RAPD analysis was widely used to identify markers tightly linked to blast resistance genes (Araújo et al 2002, Araújo et al 2004. Bulked segregant analysis by Michelmore et al (1991) was used for increasing the efficiency of RAPD markers linked to blast resistance gene to M. oryzae in rice whereas Chesquière et al (1997)…”

Section: Introductionmentioning

confidence: 99%

Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population

Araújo¹,

Prabhu²,

Pereira³

et al. 2010

CBAB

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A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS16 2072 ('AGGGGGTTCC') at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC 3 F 1 population derived from another cross between a susceptible cultivar IAC 201 and SC09. The marker OPS16 showed efficiency of 86.9%, when six resistant and two susceptible plants were considered as negatives in RAPD analysis.

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RAPD Marker Linked to a Gene Conferring Resistance to Race IB-9 of Pyricularia grisea in a Somaclone of the Rice Cultivar Araguaia

Cited by 2 publications

References 27 publications

Marcadores RAPD para detecção de resistência à ferrugem-asiática-da-soja

Marcadores RAPD para detecção de resistência à ferrugem-asiática-da-soja

Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population

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