RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato (Solanum tuberosum L.)

Takemori, Naoki; Shinoda, Kyoichi; Kadotani, Naoto

doi:10.1007/bf00234139

Cited by 27 publications

(14 citation statements)

References 23 publications

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“…RAPD markers have been used to confirm somatic hybrids of Solanum tuberosum and S. brevidens (Xu et al, 1993), those in the dihaploid breeding of potato (Takemori et al, 1994) and seedless triploids of 'Nova' tangelo + 'Succari' sweet orange . RAPD markers produced from the parental species were used to identify interspecific somatic hybrids in this experiment.…”

Section: Interspecific Somatic Hybrids Identified By Rapdmentioning

confidence: 99%

An interspecific somatic hybrid between Actinidia chinensis and Actinidia kolomikta and its chilling tolerance

Zunan

Wan

Han

2004

Plant Cell Tiss Organ Cult

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Protoplasts isolated from cotyledon-derived callus of Actinidia chinensis Planch. var. chinensis (2N ¼ 2x ¼ 58) were fused with mesophyll protoplasts of Actiniadia kolomikta (Maxim. et Rupr.) Maxim (2N ¼ 2x ¼ 58) using a PEG method. Plantlets were regenerated from the fusion product clone 11. RAPD analyses, chromosome numbers of root tip cells and fluorescence peak position of leaf nuclei confirmed that clone 11 was an interspecific somatic hybrid (2N ¼ 4x ¼ 116) between A. chinensis and A. kolomikta. The chilling tolerance of the somatic hybrid was tested with in vitro leaves at low temperatures. Based on data of leaf thickness, electroconductivity, proline levels, malondialdehyde content and activity of superoxide dismutase, dendrogram cluster analysis suggested that the interspecific somatic hybrid was similar to A. kolomikta, and might have a higher capacity of cold resistance than A. chinensis.

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Section: Interspecific Somatic Hybrids Identified By Rapdmentioning

confidence: 99%

An interspecific somatic hybrid between Actinidia chinensis and Actinidia kolomikta and its chilling tolerance

Zunan

Wan

Han

2004

Plant Cell Tiss Organ Cult

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“…The primer OPAC2 gave two specific bands to three species namely; N. triganophylla, N. benthamiana and N. repanda (Table 2). Species-specific RAPD patterns has been developed and used to confirm hybridity in potato (Takemori et al, 1994) and intergeneric hybrids of Sacharum and Erainthus (Nair et al, 2002).…”

Section: Species Specific Markersmentioning

confidence: 99%

Molecular diversity in the genus Nicotiana as revealed by randomly amplified polymorphic DNA

Raju

Sheshumadhav

Murthy

2008

Physiol Mol Biol Plants

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The genus Nicotiana consists of 64 recognized species of which, only two species, tabacum and rustica are cultivated extensively. Wild Nicotiana species are storehouses of genes for several diseases and pests, besides genes for several important phytochemicals and quality traits, which are not present in cultivated varieties. Randomly amplified polymorphic DNA (RAPD) analysis was used to determine the degree of genetic variation in the genus Nicotiana and to develop species specific markers. Twenty two species and two interspecific hybrids were analyzed by using 18 random decamer primers. Genetic polymorphism abounds among the wild species of genus Nicotiana (99.5 %) as evidenced by the high degree of polymorphism in RAPD profiles. The pairwise similarity measures in the species of subgenus Rustica was 0.252 whereas in the subgenus Tabacum was 0.189, suggesting that there was significant diversity among the species of these subgenera. In the species of subgenus Petunioides, the range of pairwise similarity measures was 0.128 to 0.941. The clustering pattern coincided with the traditional classification of Nicotiana species. All the primers generated specific bands in the various species. Thirty six species-specific markers identified in the present study will be useful in interspecific breeding programs.

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“…This technique is a tool for somatic hybrid characterization (Baird et al, 1992;Yong-Sheng, 1993;Takemori, 1994;Rasmussen and Rasmussen 1995;Penner et al, 1996;Henn et al, 1998;Rokka et al, 1998).…”

Section: Rapd Analysismentioning

confidence: 99%

Híbridos somáticos obtenidos por fusión de protoplastos entre Solanum tuberosum L. subsp. tuberosum y la especie silvestre Solanum circaeifolium Bitter

et al. 2008

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Con la finalidad de obtener híbridos somáticos interespecíficos, se fusionaron protoplastos de la especie tetraploide Solanum tuberosum y de la especie silvestre diploide Solanum circaeifolium utilizando polietilenglicol. Los productos de fusión fueron cultivados en el medio V-KM suplementado con albúmina de suero bovino. Las primeras divisiones celulares ocurrieron a los 3 a 4 días de cultivo. Después de la formación de colonias se observó una rápida proliferación de callos, a partir de los cuales se regeneraron 19 plantas. El análisis molecular usando RAPD, confirmó que los regenerantes presentaban segmentos de ADN de ambos parentales, sugiriendo su posible naturaleza de híbridos somáticos. Las observaciones del número de cromosomas indicaron que todos los híbridos fueron aneuploides. En condiciones de invernadero, los regenerantes derivados de la fusión de protoplastos, mostraron características morfológicas intermedias entre las líneas parentales. Este estudio muestra la producción de híbridos somáticos de papa con el método de fusión presentado.

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RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato (Solanum tuberosum L.)

Cited by 27 publications

References 23 publications

An interspecific somatic hybrid between Actinidia chinensis and Actinidia kolomikta and its chilling tolerance

An interspecific somatic hybrid between Actinidia chinensis and Actinidia kolomikta and its chilling tolerance

Molecular diversity in the genus Nicotiana as revealed by randomly amplified polymorphic DNA

Híbridos somáticos obtenidos por fusión de protoplastos entre Solanum tuberosum L. subsp. tuberosum y la especie silvestre Solanum circaeifolium Bitter

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